Directed evolution of cytochrome P450 enzymes for biocatalysis: Exploiting the catalytic versatility of enzymes with relaxed substrate specificity

Biochemical Journal

Volumn 467, Issue 1, 2015, Pages 1-15

  Behrendorff, James B Y H ^a   Huang, Weiliang ^a   Gillam, Elizabeth M J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Biocatalysis; Cytochrome P450; Directed evolution; DNA shuffling; Drug discovery; Substrate ambiguity

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 ENZYME; DRUG METABOLIZING ENZYME; ENZYME; UNCLASSIFIED DRUG; XENOBIOTIC AGENT; BACTERIAL PROTEIN; COENZYME; RECOMBINANT PROTEIN;

BIOCATALYSIS; BIOCATALYST; BIOTECHNOLOGY; DRUG DEVELOPMENT; ENZYME SPECIFICITY; HUMAN; METABOLOME; MICROBIAL METABOLISM; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; REVIEW; ANIMAL; BIOTRANSFORMATION; CHEMICAL STRUCTURE; CHEMISTRY; COENZYME; DIRECTED MOLECULAR EVOLUTION; DRUG DESIGN; ENZYME STABILITY; GENETICS; METABOLISM; PROTEIN CONFORMATION; TRENDS;

ANIMALS; BACTERIAL PROTEINS; BIOCATALYSIS; BIOTRANSFORMATION; COENZYMES; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; DRUG DESIGN; ENZYME STABILITY; HUMANS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; XENOBIOTICS;

EID: 84929942770     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20141493     Document Type: Review

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