Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries

Journal of Biotechnology

Volumn 139, Issue 1, 2009, Pages 12-18

  Zhang, Zhi Gang ^a,b   Liu, Yan ^a   Guengerich, F Peter ^c   Matse, Johannes H ^c   Chen, Jun ^d   Wu, Zhong Liu ^a  

^a CHENGDU INSTITUTE OF BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d CHENGDU MEDICAL COLLEGE   (China)

Author keywords

CYP2A6; Cytochrome P450; High throughput screening; Indigo; Indole hydroxylation; Random mutagenesis

Indexed keywords

CYP2A6; CYTOCHROME P450; HIGH-THROUGHPUT SCREENING; INDIGO; INDOLE HYDROXYLATION; RANDOM MUTAGENESIS;

AMINES; AMINO ACIDS; CHEMICAL REACTIONS; ENZYMES; MUTAGENESIS; ORGANIC ACIDS;

HYDROXYLATION;

4 CHLOROINDOLE; AMINO ACID; CYTOCHROME P450 2A6; DYE; INDOLE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BIOTRANSFORMATION; CATALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; GENE MUTATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; MUTAGENESIS; NONHUMAN; PIGMENTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ARYL HYDROCARBON HYDROXYLASES; DATABASES, PROTEIN; ESCHERICHIA COLI; HUMANS; HYDROXYLATION; INDOLES; KINETICS; MEMBRANES; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTAGENESIS; SEQUENCE ANALYSIS, PROTEIN;

EID: 57349106499     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.09.010     Document Type: Article

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