Expansion of substrate specificity of cytochrome P450 2A6 by random and site-directed mutagenesis

Journal of Biological Chemistry

Volumn 280, Issue 49, 2005, Pages 41090-41100

  Wu, Zhong Liu ^a   Podust, Larissa M ^a   Guengerich, F Peter ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; HYDROGEN; MUTAGENESIS; OXIDATION; SUBSTRATES;

CYTOCHROMOE P450 2A6; INDOLE COMPOUNDS;

BIOCHEMISTRY;

4 BENZYLOXY 1H INDOL 5 OL; 4 BENZYLOXY 1H INDOL 6 OL; 4 BENZYLOXY 1H INDOL 7 OL; 4 BENZYLOXY 1H INDOLE 6,7 DIONE; 4 BENZYLOXY 2 (4' BENZYLOXY 1',7' DIHYDRO 7' OXO 6'H INDOL 6' YLIDENE)INDOLIN 3 ONE; 4 BENZYLOXYINDOLE; 5 BENZYLOXYINDOLE; 5,5' DIBENZYLOXYINDIRUBIN; AMINO ACID; ASPARAGINE; COUMARIN; CYTOCHROME P450 2A6; GLUTAMINE; INDIRUBIN; INDOLE DERIVATIVE; ISOLEUCINE; MUTANT PROTEIN; OXYGEN; PYRROLE DERIVATIVE; QUINONE DERIVATIVE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME SPECIFICITY; HUMAN; HYDROGEN BOND; NONHUMAN; OXIDATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ARYL HYDROCARBON HYDROXYLASES; BENZYL COMPOUNDS; BINDING SITES; COUMARINS; CRYSTALLIZATION; GENE LIBRARY; HUMANS; HYDROGEN BONDING; INDOLES; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MIXED FUNCTION OXYGENASES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 28844494791     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M508182200     Document Type: Article

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