Testosterone 15β-hydroxylation by solvent tolerant Pseudomonas putida S12

Journal of Biotechnology

Volumn 131, Issue 2, 2007, Pages 205-208

  Ruijssenaars, Harald J ^a   Sperling, Eric M G M ^b   Wiegerinck, Peter H G ^b   Brands, Ferry T L ^b   Wery, Jan ^a   de Bont, Jan A M ^a  

^a TNO   (Netherlands)

^b NV ORGANON   (Netherlands)

Author keywords

CYP106A2; Fer; Pseudomonas putida; Solvent tolerance; Steroid synthesis; Whole cell biocatalyst

Indexed keywords

PSEUDOMONAS PUTIDA; SOLVENT TOLERANCE; STEROID SYNTHESIS; WHOLE CELL BIOCATALYSTS;

BIOCATALYSTS; ENZYME KINETICS; HYDROXYLATION; OPTIMIZATION; SOLVENTS;

DRUG PRODUCTS;

15 BETA HYDROXYLASE; BACTERIAL PROTEIN; CYTOCHROME P450; ORGANIC SOLVENT; STEROID MONOOXYGENASE; TESTOSTERONE; THREONINE; UNCLASSIFIED DRUG; VALINE; 15BETA HYDROXYLASE CYP106A2, BACILLUS MEGATERIUM; 15BETA-HYDROXYLASE CYP106A2, BACILLUS MEGATERIUM; SOLVENT;

ARTICLE; BACILLUS SUBTILIS; BIOCATALYST; BIOTRANSFORMATION; CHEMICAL STRUCTURE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSEUDOMONAS PUTIDA; STEROID HYDROXYLATION; STEROIDOGENESIS; BIOLOGICAL MODEL; ENZYME SPECIFICITY; EVALUATION; GENE EXPRESSION REGULATION; GENETICS; HYDROXYLATION; METABOLISM; SITE DIRECTED MUTAGENESIS; TRANSGENIC ORGANISM;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS; PSEUDOMONAS PUTIDA;

BACTERIAL PROTEINS; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION REGULATION, BACTERIAL; HYDROXYLATION; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; ORGANISMS, GENETICALLY MODIFIED; PSEUDOMONAS PUTIDA; SOLVENTS; SUBSTRATE SPECIFICITY; TESTOSTERONE;

EID: 34547536911     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.06.007     Document Type: Article

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