Engineering bacterial cytochrome P450 (P450) BM3 into a prototype with human P450 enzyme activity using indigo formation

Drug Metabolism and Disposition

Volumn 38, Issue 5, 2010, Pages 732-739

  Park, Sun Ha ^a   Kim, Dong Hyun ^a   Dooil, Kim ^b   Kim, Dae Hwan ^a   Jung, Heung Chae ^b   Pan, Jae Gu ^b   Taeho, Ahn ^a   Donghak, Kim ^c   Yun, Chul Ho ^a  

^a Chonnam National University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c Konkuk University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

3 HYDROXYCOUMARIN; 7 ETHOXYCOUMARIN; CHLORZOXAZONE; COUMARIN; CYTOCHROME P450 BM3; ETHOXYRESORUFIN; FLUORESCENT DYE; INDOLE; PHENACETIN;

ARTICLE; BINDING SITE; CATALYSIS; CELL LEVEL; CHEMICAL MODIFICATION; COLORIMETRY; CORRELATION FUNCTION; DNA LIBRARY; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME SUBSTRATE; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HYDROXYLATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLITE; MOLECULAR DYNAMICS; MUTAGENESIS; MUTANT; NONHUMAN; OXIDATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WHOLE CELL;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BIOCATALYSIS; CARBON; CHLORZOXAZONE; COUMARINS; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME STABILITY; ESCHERICHIA COLI; HEME; HUMANS; INDOLES; KINETICS; MOLECULAR DYNAMICS SIMULATION; NADPH-FERRIHEMOPROTEIN REDUCTASE; NITROPHENOLS; OXAZINES; OXIDATION-REDUCTION; PHENACETIN; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC; UMBELLIFERONES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77950941685     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.109.030759     Document Type: Article

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