A shuffled CYP2C library with a high degree of structural integrity and functional versatility

Archives of Biochemistry and Biophysics

Volumn 467, Issue 2, 2007, Pages 193-205

  Huang, Weiliang ^a   Johnston, Wayne A ^a   Hayes, Martin A ^b   De Voss, James J ^a   Gillam, Elizabeth M J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b ASTRAZENECA R AND D   (Sweden)

Author keywords

Biocatalysts; CYP2C; Cytochrome P450; Directed evolution; DNA shuffling

Indexed keywords

CYTOCHROME P450 2C; CYTOCHROME P450 2C18; CYTOCHROME P450 2C19; CYTOCHROME P450 2C8; CYTOCHROME P450 2C9; DICLOFENAC; HEMOPROTEIN; INDOLE; LUCIFERIN;

ANIMAL CELL; ARTICLE; BIOCATALYST; CONTROLLED STUDY; DNA SHUFFLING; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROXYLATION; MULTIGENE FAMILY; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; XENOBIOTIC METABOLISM;

CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; PEPTIDE LIBRARY;

MAMMALIA;

EID: 35748939419     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2007.08.023     Document Type: Article

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