Bioreactor systems in drug metabolism: Synthesis of cytochrome P450-generated metabolites

Metabolic Engineering

Volumn 2, Issue 2, 2000, Pages 115-125

  Rushmore, Thomas H ^a   Reider, Paul J ^a   Slaughter, Don ^a   Assang, Carol ^a   Shou, Magang ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

15BETA HYDROXYTESTOSTERONE; 4' HYDROXYDICLOFENAC; 6BETA HYDROXYTESTOSTERONE; BIOREACTOR; CYTOCHROME P450; DIAZEPAM; DICLOFENAC; DRUG METABOLISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSECT CELL; METABOLITE; NORDAZEPAM; OXAZEPAM; REACTION MECHANISM; SUSPENSION CELL CULTURE; TEMAZEPAM; TESTOSTERONE;

INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0034044068     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.2000.0147     Document Type: Article

References (20)

1. Chen L., Buters J. T. M., Hardwick J. P., Tamura S., Penman B. W., Gonzalez F. J., Crespi C. H. Coexpression of cytochrome P4502A6 and human NADPH-P450 oxidoreductase in the baculovirus system. Drug Metab. Dispos. 25:1997;399-405.
2. Crespi C. L., Langenbach R., Penman B. W. The development of a panel of human cell lines expressing specific human cytochrome P450 cDNAs. Mendelsohn M. L., Albertini R. L. Mutation and the Environment. 1990;97-106 Wiley-Liss, New York.
3. Gergel D., Misik V., Riesz P., Cederbaum A. I. Inhibition of rat and human cytochrome P4502E1 catalytic activity and reactive oxygen radical formation by nitric oxide. Arch. Biochem. Biophys. 337:1997;239-250.
4. Gonzalez F. J., Aoyama T., Gelboin H. V. Expression of mammalian cytochrome P450 using vaccinia virus. Methods Enzymol. 206:1991a;85-92.
5. Gonzalez F. J., Kimura S., Tamura S., Gelboin H. V. Expression of mammalian cytochrome P450 using baculovirus. Methods Enzymol. 206:1991b;93-99.
6. Iwata H., Fujita K., Kushida H., Suzuki A., Konno Y., Nakamura K., Fujino A., Kamataki T. High catalytic activity of human cytochrome P450 co-expressed with human NADPH-cytochrome P450 reductase in Escherichia coli. Biochem. Pharmacol. 55:1998;1315-1325.
7. Lee C. A., Kost T. A., Serabjit-Singh C. J. Recombinant baculovirus strategy for coexpression of functional human cytochrome P450 and P450 reductase. Methods Enzymol. 272:1996;86-95.
8. Lee C. A., Kadwell S. H., Kost T. A., Serabjit-Singh C. J. CYP3A4 expression by insect cells infected with a recombinant baculovirus containing both CYP3A4 and human NADPH- cytochrome P450 reductase is catalytically similar to human liver microsomal CYP3A4. Arch. Biochem. Biophys. 319:1995;157-167.
9. Li Y. C., Chaing J. Y. The expression of catalytically active cholesterol 7α-hydroxylase in Escherichia coli. J. Biol. Chem. 266:1991;19186-19191.
10. Lin H. L., Roberts E. S., Hollenberg P. F. Heterologous expression of rat P4502E1 in a mammalian cell line: In situ metabolism and cytotoxicity of N-nitrosodimethylamine. Carcinogenesis. 19:1998;321-329.
11. Oeda K., Sakaki T., Ohkawa H. Expression of rat liver cytochrome P-450MC cDNA in Saccharomyces cerevisiae. DNA Cell Biol. 4:1985;167-175.
12. Omura T., Sato R. The carbon dioxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J. Biol. Chem. 239:1964;2370-2378.
13. Ong C. E., Miners J. O., Birkett D. J., Bhasker C. R. Baculovirus-mediated expression of cytochrome P4502C8 and human NADPH-cytochrome P450 reductase: Optimization of protein expression. Xenobiotica. 28:1998;137-152.
14. Overton L. K., Kost T. A. Potential application of insect cell-based expression systems in the bio/pharmaceutical industry. Overton L. K., Kost T. A. Baculovirus Expression Systems and Biopesticides. 1995;233-242 Wiley-Liss, New York.
15. Rushmore T. H., Davis M. A., Cui D. H., Shou M. Large scale production of metabolites from parent drugs by suspension cultures of Sf21 cells that overexpress CYP2C9. ISSX Proc. 12:1997;162.
16. Sambrook J., Fritsch E. F., Maniatis T. J. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, New York.
17. Shou M., Gonzalez F. J., Gelboin H. V. Stereoselective epoxidation and hydration at the K-region of polycyclic aromatic hydrocarbons by cDNA-expressed cytochromes P450 1A1, 1A2, and epoxide hydrolase. Biochemistry. 35:1996;15807-15813.
18. Webster R., Pacey M., Winchester T., Johnson P., Jezequel S. Microbial oxidative metabolism of diclofenac: Production of 4′-hydroxydiclofenac using Epiccocum nigrum IMI354292. Appl. Microbiol. Biotechnol. 49:1998;371-376.
19. Yang T. J., Shou M., Korzekwa K. R., Gonzalez F. J., Gelboin H. V., Ang S. K. Role of cDNA-expressed human cytochromes P450 in the metabolism of diazepam. Biochem. Pharmacol. 55:1998;889-896.
20. Zeng L., Kassel D. B. Developments of a fully automated parallel HPLC/mass spectrometry system for the analytical characterization and preparative purification of combinatorial libraries. Anal. Chem. 70:1998;4380-4388.