Re-engineering cytochrome P450 2B11dH for enhanced metabolism of several substrates including the anti-cancer prodrugs cyclophosphamide and ifosfamide

Archives of Biochemistry and Biophysics

Volumn 458, Issue 2, 2007, Pages 167-174

  Sun, Ling ^a   Chen, Chong S ^b   Waxman, David J ^b   Liu, Hong ^c   Halpert, James R ^a   Kumar, Santosh ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

^b Boston University   (United States)

^c SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Cytochrome P450; Enzyme catalysis; P450 2B11; P450 engineering; Site directed mutagenesis; Structure function relationships

Indexed keywords

CYCLOPHOSPHAMIDE; CYTOCHROME P450; CYTOCHROME P450 CYP2B11; IFOSFAMIDE; TESTOSTERONE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOENGINEERING; CONTROLLED STUDY; ENZYME MECHANISM; ENZYME SUBSTRATE; HYDROXYLATION; MUTANT; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN METABOLISM; RAT;

AMINO ACID SUBSTITUTION; ANIMALS; ANTINEOPLASTIC AGENTS, ALKYLATING; ARYL HYDROCARBON HYDROXYLASES; COUMARINS; CYCLOPHOSPHAMIDE; DIRECTED MOLECULAR EVOLUTION; HYDROXYLATION; IFOSFAMIDE; LIVER; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXAZINES; OXIDATION-REDUCTION; PRODRUGS; RATS; TESTOSTERONE;

EID: 33846908749     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2006.12.021     Document Type: Article

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