High throughput assay for cytochrome P450 BM3 for screening libraries of substrates and combinatorial mutants

Biosensors and Bioelectronics

Volumn 17, Issue 1-2, 2002, Pages 119-131

  Tsotsou, Georgia Eleni ^a   Cass, Anthony Edward George ^a   Gilardi, Gianfranco ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

NAD(P)(H) dependent oxidoreductases; P450 (BM3) specificity; Whole cell screening

Indexed keywords

BIOASSAY; BIODEGRADATION; CATALYSIS; ENZYMES; ESCHERICHIA COLI; METABOLISM; OXIDATION;

MUTANTS;

BIOSENSORS;

ARACHIDONIC ACID; CAS 1609; CYTOCHROME P450; DODECYL SULFATE SODIUM; FURAZAN DERIVATIVE; LAURIC ACID; MB 940 C; MV 332 B; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; S 77 A; S 77 B; SN 10; TETRACHLOROETHANE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS MEGATERIUM; CELL LYSATE; DEGRADATION; ENZYME ASSAY; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN; OXIDATION; PERFORMANCE; PROTEIN EXPRESSION; SCREENING; TECHNIQUE;

BACTERIAL PROTEINS; COMBINATORIAL CHEMISTRY TECHNIQUES; CYTOCHROME P-450 ENZYME SYSTEM; MIXED FUNCTION OXYGENASES; MUTATION; NADP; OXIDATION-REDUCTION; PEPTIDE LIBRARY;

ESCHERICHIA COLI;

EID: 0036136872     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(01)00285-8     Document Type: Article

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