Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase

Nature Biotechnology

Volumn 20, Issue 11, 2002, Pages 1135-1139

  Glieder, Anton ^a   Farinas, Edgardo T ^b   Arnold, Frances H ^b  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DERIVATIVE; ALKANE; ALKANE 1 MONOOXYGENASE; BACTERIAL PROTEIN; CYTOCHROME P450; MIXED FUNCTION OXIDASE; RECOMBINANT PROTEIN; FATTY ACID; FATTY ACID MONOOXYGENASE; METHANE MONOOXYGENASE; NONHEME IRON ALKANE MONOOXYGENASE; OXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS MEGATERIUM; BIOSYNTHESIS; CATALYSIS; CLASSIFICATION; ENZYME ACTIVATION; ENZYMOLOGY; GENETICS; HYDROXYLATION; METABOLISM; METHODOLOGY; MOLECULAR EVOLUTION; OXIDATION REDUCTION REACTION; PROTEOMICS; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY; SITE DIRECTED MUTAGENESIS; SOLUBILITY; SPECIES DIFFERENCE; BIOREMEDIATION; CHEMICAL BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; ROOM TEMPERATURE; SYNTHESIS; WILD TYPE;

ALCOHOLS; ALKANE 1-MONOOXYGENASE; ALKANES; BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CATALYSIS; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME ACTIVATION; EVOLUTION, MOLECULAR; HYDROXYLATION; MIXED FUNCTION OXYGENASES; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; PROTEOMICS; QUALITY CONTROL; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SOLUBILITY; SPECIES SPECIFICITY;

ALCOHOLS; BIOCATALYSTS; BIOREMEDIATION; ENZYMES; FATTY ACIDS; HYDROXYLATION; OXIDATION; PARAFFINS;

HIGH SPIN SHIFT;

BIOTECHNOLOGY;

EID: 0036842594     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt744     Document Type: Article

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