Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D 3 hydroxylase

Journal of Biological Chemistry

Volumn 285, Issue 41, 2010, Pages 31193-31201

  Yasutake, Yoshiaki ^a   Fujii, Yoshikazu ^b,c   Nishioka, Taiki ^b   Cheon, Woo Kwang ^a,d   Arisawa, Akira ^c   Tamura, Tomohiro ^a,b  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

^c MERCIAN CORPORATION   (Japan)

^d Keimyung University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINDING ENERGY; BIOLOGY; CONFORMATIONS; HYDROXYLATION; PORPHYRINS;

ANTIPARALLEL ORIENTATIONS; BEFORE AND AFTER; BINDING MODES; BIOLOGICAL FUNCTIONS; CONFORMATIONAL EQUILIBRIUM; CYTOCHROME P450; DIRECTED EVOLUTION; FOUR AMINO ACIDS; HEME POCKETS; HYDROXYLASE ACTIVITY; HYDROXYLASES; OPEN CONFORMATION; PSEUDONOCARDIA; REGIO-SELECTIVE; SPECTROSCOPIC DATA; STRUCTURAL EVIDENCE; SUBSTRATE-BOUND; SUBSTRATE-FREE; VITAMIN-D; WILD TYPES;

SUBSTRATES;

AMINO ACID; CALCIDIOL 1 MONOOXYGENASE; CALCIFEDIOL; COLECALCIFEROL; COLECALCIFEROL HYDROXYLASE; CYTOCHROME P450; HEME; MUTANT PROTEIN; SECOSTEROID; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CALCITRIOL; STEROID MONOOXYGENASE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; HORMONE BINDING; HYDROXYLATION; NONHUMAN; PRIORITY JOURNAL; WILD TYPE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PROTEIN SECONDARY STRUCTURE; PSEUDOMONAS; X RAY CRYSTALLOGRAPHY;

PSEUDONOCARDIA AUTOTROPHICA;

BACTERIAL PROTEINS; CALCITRIOL; CHOLECALCIFEROL; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MUTATION; PROTEIN STRUCTURE, SECONDARY; PSEUDOMONAS; STEROID HYDROXYLASES;

EID: 77957814994     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.147009     Document Type: Article

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