Novel approaches for discovering industrial enzymes

Current Opinion in Microbiology

Volumn 2, Issue 3, 1999, Pages 241-245

  Marrs, Barry ^a   Delagrave, Simon ^a   Murphy, Dennis ^a  

^a Hercules Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIODIVERSITY; BIOTECHNOLOGY; CATALYSIS; CHEMICAL MODIFICATION; ENZYME PURIFICATION; PHAGE DISPLAY; REVIEW; STEREOSPECIFICITY;

EID: 0033153175     PISSN: 13695274     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-5274(99)80042-3     Document Type: Article

References (40)

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23. Nevill-Manning CG, Wu TD, Brutlag DL: Highly specific protein sequence motifs for genome analysis. Proc Natl Acad Sci USA 1998, 95:5865-5871. A good example of bioinformatics applied to the search for new enzymes distantly related to enzymes of known function.
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32. +2 on mutation rates.
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34. Kuchner O, Arnold FH: Strategies for the in vitro evolution of protein function: Enzyme evolution by random recombination of improved sequences. J Mol Biol 1997, 272:336-347. Required reading for in vitro evolution via DNA shuffling.
35. Shao Z, Zhao H, Giver L, Arnold FH: Random-priming in vitro recombination: An effective tool for directed evolution. Description of RPR for DNA shuffling. Nucleic Acids Res 1998, 26:681-683. A description of RPR for DNA shuffling. This technique differs from Stemmer's [29,30] because the generation of oligomers occurs by synthesis (i.e. polymerization) of duplex DNA rather than fragmentation of existing duplex DNA. It differs from StEP [36••] in that the primers are random oligomers.
36. Zhao H, Giver L, Shao Z, Affholter JA, Arnold FH: Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat Biotechnol 1998, 16:258-261. Description of StEP for DNA shuffling. This technique differs from Stemmer's [29,30] because the generation of oligomers occurs by synthesis (i.e. polymerization) of duplex DNA rather than fragmentation of existing of duplex DNA. It differs from RPR [35••] in that only one pair of primers is used.
37. Matsuura T, Miyai K, Trakulnaleamsai S, Yomo T, Shima Y, Miki S, Yamamoto K, Urabe I: Evolutionary molecular engineering by random elongation mutagenesis. Nature Biotechnol 1999, 17:58-61.
38. Pedersen H, Holder S, Sutherlin DP, Schwitter U, King DS, Schultz PG: A method for directed evolution and functional cloning of enzymes. Proc Natl Acad Sci USA 1998, 95:10523-10528. The system described in this article is complex but is one of the first successful application of phage display to the selection of catalysts from a complex mixture.
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