Molecular evolution by staggered extension process (StEP) in vitro recombination

Nature Biotechnology

Volumn 16, Issue 3, 1998, Pages 258-261

  Zhao, Huimin ^a   Giver, Lori ^a   Shao, Zhixin ^a   Affholter, Joseph A ^b   Arnold, Frances H ^a  

^a California Institute of Technology   (United States)

^b DOW CHEMICAL COMPANY   (United States)

Author keywords

Directed evolution; Random mutagenesis; Subtilisin

Indexed keywords

SUBTILISIN;

ARTICLE; DNA LIBRARY; GENETIC RECOMBINATION; MOLECULAR EVOLUTION; MUTAGENESIS; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACILLUS SUBTILIS; EVOLUTION, MOLECULAR; GENE LIBRARY; MUTAGENESIS; MUTATION; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; SUBTILISINS; TEMPLATES, GENETIC;

EID: 0031909113     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0398-258     Document Type: Article

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