Directed evolution of the Actinomycete Cytochrome P450 MoxA (CYP105) for enhanced activity

Bioscience, Biotechnology and Biochemistry

Volumn 73, Issue 9, 2009, Pages 1922-1927

  Kabumoto, Hiroki ^a   Miyazaki, Kentaro ^b,c   Arisawa, Akira ^a  

^a MERCIAN CORPORATION   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Actinomycete; Cytochrome P450; Directed evolution; Hydroxylation; Substrate specificity

Indexed keywords

7-ETHOXYCOUMARIN; ACTINOMYCETE; ACTIVE SITE; AMINO ACID SUBSTITUTION; CYTOCHROME P450; DICLOFENAC; DIRECTED EVOLUTION; MEDICINAL APPLICATIONS; PRACTICAL USE; RANDOM MUTAGENESIS; SINGLE MUTATION; SUBSTRATE RECOGNITION SITES; SUBSTRATE SPECIFICITY; THREE-DIMENSIONAL STRUCTURE; WILD TYPES;

AMINES; AMINO ACIDS; ENZYMES; FATTY ACIDS; HYDROXYLATION; NICKEL COMPOUNDS; ORGANIC ACIDS; PLANTS (BOTANY);

SUBSTRATES;

CYTOCHROME P450; DICLOFENAC; FLAVANONE DERIVATIVE; NARINGENIN; PRIMER DNA;

ACTINOBACTERIA; ARTICLE; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; EVOLUTION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; METABOLISM; MUTAGENESIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION;

ACTINOBACTERIA; BASE SEQUENCE; BIOCATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 ENZYME SYSTEM; DICLOFENAC; DNA PRIMERS; EVOLUTION; FLAVANONES; HYDROXYLATION; MODELS, MOLECULAR; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

ACTINOBACTERIA; NONOMURAEA RECTICATENA;

EID: 70349694356     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.90013     Document Type: Article

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