Libraries of hybrid proteins from distantly related sequences

Nature Biotechnology

Volumn 19, Issue 5, 2001, Pages 456-460

  Sieber, Volker ^a   Martinez, Carlos A ^a   Arnold, Frances H ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; ENZYMES; GENES;

HYBRID PROTEINS;

PROTEINS;

CHLORAMPHENICOL ACETYLTRANSFERASE; CYTOCHROME P450 1A2; ETHOXYRESORUFIN; HEME; HYBRID PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; GENE FUSION; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN VARIANT; RAT; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SOLUBILITY;

AMINO ACID SEQUENCE; ARTIFICIAL GENE FUSION; BACTERIAL PROTEINS; BASE SEQUENCE; CYTOCHROME P-450 CYP1A2; CYTOCHROME P-450 ENZYME SYSTEM; GENE LIBRARY; HUMANS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY; SOLUBILITY;

BACTERIA (MICROORGANISMS); FELIS CATUS;

EID: 0035025314     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/88129     Document Type: Article

References (24)

1. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
2. Molecular evolution by staggered extension process (StEP) in vitro recombination
3. Random-priming in vitro recombination: An effective tool for directed evolution
4. Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair
5. Directed evolution of a fungal peroxidase
6. A combinatorial approach to hybrid enzymes independent of DNA homology
7. A novel structural basis for membrane association of a protein: Construction of a chimeric soluble mutant of (S)-mandelate dehydrogenase from Pseudomonas putida
8. Design of a novel P450: A functional bacterial-human cytochrome P450 chimera
9. A hierarchical approach to protein molecular evolution
10. Cloning and isolation of human cytochrome P-450 cDNAs homologous to dioxin-inducible rabbit mRNAs encoding P-450-4 and P-450-6
11. Partial characterization of a barbiturate-induced cytochrome P-450- dependent fatty acid monooxygenase from Bacillus megaterium
12. Random circular permutation of genes and expressed polypeptide chains: Application of the method to the catalytic chains of aspartate transcarbamoylase
13. Circular permutation and receptor insertion within green fluorescence proteins
14. A simple in vivo assay for increased protein solubility
15. Chloramphenicol acetyltransferase
16. Spectral analysis of cytochromes P450
17. Catalytic assays for human cytochrome P450
18. Recombinant human cytochrome P450 1A2 and an N-terminal-truncated form: Construction, purification, aggregation properties, and interactions with flavodoxin, ferredoxin, and NADPH-cytochrome P450 reductase
19. Chimeras of the human cytochrome P450 1A family produced in yeast. Accumulation in microsomal membranes, enzyme kinetics and stability
20. Evolution of protein function by domain swapping
21. Drug metabolism by Escherichia coli expressing human cytochromes P450
22. Regulation of heme biosynthesis in Escherichia coli
23. Expression of eukaryotic cytochromes P450 in E. Coli