Purification, characterization, and directed evolution study of a vitamin D3 hydroxylase from Pseudonocardia autotrophica

Biochemical and Biophysical Research Communications

Volumn 385, Issue 2, 2009, Pages 170-175

  Fujii, Yoshikazu ^a,c   Kabumoto, Hiroki ^a   Nishimura, Kenji ^a   Fujii, Tadashi ^a   Yanai, Satoshi ^a   Takeda, Koji ^a   Tamura, Noriko ^b   Arisawa, Akira ^a   Tamura, Tomohiro ^b,c  

^a MERCIAN CORPORATION   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

Author keywords

1 ,25 Dihydroxyvitamin D3; 25 Hydroxyvitamin D3; Cytochrome P450 monooxygenase; Pseudonocardia autotrophica; Vdh; Vitamin D3 hydroxylase

Indexed keywords

AMINO ACID; BACTERIAL ENZYME; COLECALCIFEROL; COLECALCIFEROL HYDROXYLASE; CYTOCHROME P450; CYTOCHROME P450 107; DNA; MUTANT PROTEIN; UNCLASSIFIED DRUG; VITAMIN D3 HYDROXYLASE K1;

ACTINOBACTERIA; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; BIOTRANSFORMATION; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; GENE EXPRESSION; GENE MUTATION; HOMOZYGOTE; HYDROXYLATION; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDONOCARDIA AUTOTROPHICA; PSEUDONOCARDIACEAE; RHODOCOCCUS ERYTHROPOLIS; SITE DIRECTED MUTAGENESIS; STEREOSPECIFICITY; WILD TYPE;

ACTINOMYCETALES; AMINO ACID SEQUENCE; CLONING, MOLECULAR; CYTOCHROME P-450 CYP27A1; DIRECTED MOLECULAR EVOLUTION; MOLECULAR SEQUENCE DATA;

MAMMALIA; PSEUDONOCARDIA AUTOTROPHICA; RHODOCOCCUS ERYTHROPOLIS;

EID: 67349154411     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.05.033     Document Type: Article

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