Large-scale production and protein engineering of G protein-coupled receptors for structural studies

Frontiers in Pharmacology

Volumn 6, Issue MAR, 2015, Pages

  Milic, Dalibor ^a   Veprintsev, Dmitry B ^a,b  

^a PAUL SCHERRER INSTITUTE   (Switzerland)

^b ETH ZURICH   (Switzerland)

Author keywords

Expression; G protein coupled receptor (GPCR); Isotope labeling; Protein engineering; Purification

Indexed keywords

DETERGENT; G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; RHODOPSIN; SURFACTANT;

AMINO TERMINAL SEQUENCE; BOMBYX MORI; BOVINAE; CAENORHABDITIS ELEGANS; CARBOXY TERMINAL SEQUENCE; CELL DISRUPTION; CELL FREE PROTEIN EXPRESSION; CELL MEMBRANE; CHROMATOGRAPHY; CRYSTAL STRUCTURE; CRYSTALLIZATION; DEGLYCOSYLATION; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HALOFERAX VOLCANII; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INSECT CELL; ION EXCHANGE CHROMATOGRAPHY; KOMAGATAELLA PASTORIS; LIGAND AFFINITY CHROMATOGRAPHY; MAMMAL CELL; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POINT MUTATION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; REVIEW; RHODOBACTER SPHAEROIDES; SOLUBILIZATION; SQUID; THERMOSTABILITY; TRANSGENIC MOUSE; ULTRACENTRIFUGATION; XENOPUS LAEVIS; YEAST;

EID: 84926623484     PISSN: None     EISSN: 16639812     Source Type: Journal    
DOI: 10.3389/fphar.2015.00066     Document Type: Review

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