Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments

Biochimica et Biophysica Acta - Biomembranes

Volumn 1828, Issue 9, 2013, Pages 2182-2192

  Proverbio, Davide ^a   Roos, Christian ^a   Beyermann, Michael ^b   Orbán, Erika ^a   Dötsch, Volker ^a   Bernhard, Frank ^a  

^a GOETHE UNIVERSITY   (Germany)

^b LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

Author keywords

Cell free expression; G protein coupled receptor; Human endothelin system; Lipid screening; Nanodisc; Vasoactive peptide

Indexed keywords

ENDOTHELIN 1; ENDOTHELIN A RECEPTOR; ENDOTHELIN B RECEPTOR; ENDOTHELIN RECEPTOR AGONIST; LIPOSOME; NANODISC; CHOLESTEROL HYDROGEN SUCCINATE; DETERGENT; ESSENTIAL PHOSPHOLIPID; G PROTEIN COUPLED RECEPTOR; NANOMATERIAL; PHOSPHATE BUFFERED SALINE; PROTEORHODOPSIN; VASOACTIVE PEPTIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; ARTIFICIAL MEMBRANE; BINDING AFFINITY; CELL FREE SYSTEM; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; IN VITRO STUDY; LIGAND BINDING; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; RADIOASSAY; RECEPTOR BINDING; SURFACE PLASMON RESONANCE; 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHO-(1′-RAC-GLYCEROL); 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; 1-MYRISTOYL-2-HYDROXY-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)]; 1-PALMITOYL-2-HYDROXY-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)]; 1-PALMITOYL-2-OLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; ASO-PC; BIOSYNTHESIS; BRAIN TOTAL EXTRACT; BTE; CECF; CELL-FREE; CELL-FREE EXPRESSION; CF; CHEMISTRY; CHS; COLUMN VOLUMES; CONTINUOUS EXCHANGE CELL-FREE; CVS; DMPC; DMPG; DOPC; ESCHERICHIA COLI POLAR LIPIDS; ETA; ETB; FEEDING MIX; FM; G-PROTEIN COUPLED RECEPTOR; GENE EXPRESSION; GENETICS; HEART TOTAL LIPIDS; HTL; HUMAN; HUMAN ENDOTHELIN SYSTEM; IMAC; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; KINETICS; LIPID ANALYSIS; LMPG; LOW TEMPERATURE SDS-PAGE; LPPG; LT-SDS-PAGE; MEMBRANE SCAFFOLD PROTEIN; METABOLISM; MSP; ND; PBS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POPC; PR; PROTEIN BINDING; PROTEIN FOLDING; REACTION MIX; RED SHIFTED GREEN FLUORESCENT PROTEIN; RM; SGFP; SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESES; SOYBEAN L-Α-PHOSPHATIDYLCHOLINE; SPR;

1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHO-(1′-RAC-GLYCEROL); 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; 1-MYRISTOYL-2-HYDROXY-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)]; 1-PALMITOYL-2-HYDROXY-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)]; 1-PALMITOYL-2-OLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; ASO-PC; BRAIN TOTAL EXTRACT; BTE; CECF; CELL-FREE; CELL-FREE EXPRESSION; CF; CHOLESTERYL HEMISUCCINATE; CHS; COLUMN VOLUMES; CONTINUOUS EXCHANGE CELL-FREE; CVS; DMPC; DMPG; DOPC; ENDOTHELIN A RECEPTOR; ENDOTHELIN B RECEPTOR; EPL; ESCHERICHIA COLI POLAR LIPIDS; ETA; ETB; FEEDING MIX; FM; G-PROTEIN COUPLED RECEPTOR; GPCR; HEART TOTAL LIPIDS; HTL; HUMAN ENDOTHELIN SYSTEM; IMAC; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIPID SCREENING; LMPG; LOW TEMPERATURE SDS-PAGE; LPPG; LT-SDS-PAGE; MEMBRANE SCAFFOLD PROTEIN; MSP; NANODISC; ND; PBS; PHOSPHATE BUFFERED SALINE; POPC; PR; PROTEORHODOPSIN; REACTION MIX; RED SHIFTED GREEN FLUORESCENT PROTEIN; RM; SDS-PAGE; SGFP; SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESES; SOYBEAN L-Α-PHOSPHATIDYLCHOLINE; SPR; SURFACE PLASMON RESONANCE; VASOACTIVE PEPTIDE;

CELL-FREE SYSTEM; DETERGENTS; ENDOTHELIN-1; GENE EXPRESSION; HUMANS; KINETICS; LIPOSOMES; NANOSTRUCTURES; PROTEIN BINDING; PROTEIN FOLDING; RECEPTOR, ENDOTHELIN A; RECEPTOR, ENDOTHELIN B;

EID: 84879472640     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2013.05.031     Document Type: Article

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