Improvements in G protein-coupled receptor purification yield light stable rhodopsin crystals

Journal of Structural Biology

Volumn 156, Issue 3, 2006, Pages 497-504

  Salom, David ^a   Le Trong, Isolde ^b   Pohl, Ehmke ^c   Ballesteros, Juan A ^a   Stenkamp, Ronald E ^b   Palczewski, Krzysztof ^d   Lodowski, David T ^d  

^a Novasite Pharmaceuticals Inc   (United States)

^b Biomolecular Structure Center   (United States)

^c PAUL SCHERRER INSTITUTE   (Switzerland)

^d CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Crystallization; G protein coupled receptors (GPCRs); Membrane proteins; Photoactivation; Photoreceptor cells; Rhodopsin; Signal transduction

Indexed keywords

G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; RHODOPSIN;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLIZATION; NONHUMAN; PHOTOACTIVATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS; X RAY DIFFRACTION;

AMMONIUM SULFATE; ANIMALS; CATTLE; CHROMATOGRAPHY, AFFINITY; CRYOPRESERVATION; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; LIGANDS; LIGHT; RECEPTORS, G-PROTEIN-COUPLED; RHODOPSIN; TIME FACTORS; X-RAY DIFFRACTION;

EID: 33751019133     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2006.05.003     Document Type: Article

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