Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution

Journal of Molecular Biology

Volumn 422, Issue 3, 2012, Pages 414-428

  Schlinkmann, Karola M ^a   Hillenbrand, Matthias ^a   Rittner, Alexander ^a   Künz, Madeleine ^a   Strohner, Ralf ^b   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b MORPHOSYS AG   (Germany)

Author keywords

coevolution; in vitro DNA recombination; membrane protein expression; plasmid copy number; screening and selection

Indexed keywords

ALANINE; DETERGENT; G PROTEIN COUPLED RECEPTOR; NEUROTENSIN RECEPTOR;

ARTICLE; BACTERIAL CELL; CODON; COEVOLUTION; CONTROLLED STUDY; DNA RECOMBINATION; ESCHERICHIA COLI; FRAMESHIFT MUTATION; IN VITRO STUDY; MICELLE; MOLECULAR LIBRARY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; WILD TYPE;

ESCHERICHIA COLI; RATTUS;

EID: 84865068808     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2012.05.039     Document Type: Article

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