Purification and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli

European Journal of Biochemistry

Volumn 269, Issue 1, 2002, Pages 82-92

  Weiß, H Markus ^a,b   Grisshammer, Reinhard ^a,c  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b Aeskulab Diagnostika ^*  (Germany)

^c NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

3H ZM241385; Adenosine A2a receptor; Functional solubilization; G protein coupled receptor; Maltose binding protein fusion

Indexed keywords

4 [2 [7 AMINO 2 (2 FURYL) 1,2,4 TRIAZOLO[2,3 A][1,3,5]TRIAZIN 5 YLAMINO]ETHYL]PHENOL; 8 [4 (2 AMINOETHYLCARBAMOYL)METHOXYPHENYL] 1,3 DIPROPYLXANTHINE; ADENOSINE A2A RECEPTOR; ALANINE; CHOLESTEROL HYDROGEN SUCCINATE; LIGAND; MALTOSE BINDING PROTEIN; PROTEINASE; TRITIUM;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL MEMBRANE; BACTERIUM CULTURE; BIOPHYSICS; CARBOXY TERMINAL SEQUENCE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SOLUBILIZATION;

ESCHERICHIA COLI; HUMANS; RECEPTORS, PURINERGIC P1; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TRIAZINES; TRIAZOLES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 12244292640     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2002.02618.x     Document Type: Article

References (53)

1. Purinoceptors in the nervous system
2. Adenosine receptors: New opportunities for future drugs
3. 2A receptor gene by hypoxia in PC12 cells
4. Insight into adenosine receptor function using antisense and gene-knockout approaches
5. 2A receptors
6. 2a receptor
7. Overexpression of integral membrane proteins for structural studies
8. Heterologous expression of G-protein-coupled receptors
9. Production of G-protein-coupled receptors in yeast
10. Heterologous expression of G-protein-coupled receptors: Human opioid receptors under scrutiny
11. Projection structure of rhodopsin
12. Arrangement of rhodopsin transmembrane alpha-helices
13. Crystal structure of rhodopsin: A G protein-coupled receptor
14. 2A receptors in the rat central nervous system
15. 1 adenosine receptor from rat brain membranes
16. 1 adenosine receptor
17. 1 adenosine receptors
18. Molecular characterization of a human brain adenosine A2 receptor
19. Purification of rat neurotensin receptor expressed in Escherichia coli
20. 2a adenosine receptor
21. Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea
22. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
23. A rapid, sensitive, and specific method for the determination of protein in dilute solution
24. 5A 5-hydroxytryptamine receptor and the human β2-adrenergic receptor produced in the methylotrophic yeast Pichia Pastoris
25. 2a adenosine receptor domain specifically responsible for mediating short-term desensitization
26. 2a adenosine receptors retain ligand binding
27. 3H]-SCH 58261
28. Comparative pharmacology of human adenosine receptor subtypes - Characterization of stably transfected receptors in CHO cells
29. 2S receptor in baculovirus-infected insect cells
30. 1 adenosine receptor-G-protein complexes: Effects of receptor down-regulation and phosphorylation on coupling
31. 2A-adenosine receptors with hexahistidine and the FLAG epitope
32. Expression of a rat neurotensin receptor in Escherichia coli
33. Amino and carboxyl terminal modifications to facilitate the production and purification of a G protein-coupled receptor
34. Molecular chaperones stimulate the functional expression of the cocaine-sensitive serotonine transporter
35. Purification of recombinant porcine m2 muscarinic acetylcholine receptor from chinese hamster ovary cells
36. Expression, purification, and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide
37. Glycerol, sodium phosphate, and sodium chloride permit the solubilization and partial purification of rat hepatic alpha 1-receptors by 3-(3-cholamidylpropyl)-dimethylammonio-1-propanesulfonate
38. 2-adenosine receptor
39. 2a-adenosine Receptors: Stability and antagonist binding
40. 2a adenosine receptors: Guanine nucleotide derivative regulation in porcine striatal membranes and digitonin soluble fraction
41. Affinity purification of a somatostatin receptor-G-protein complex demonstrates specificity in receptor-G-protein coupling
42. Rhodopsin - Cholesterol interactions in bovine rod outer segment disk membranes
43. Cholesterol as modulator of receptor function
44. Quantitative evaluation of neurotensin receptor purification by immobilized metal affinity chromatography
45. Comparison of CGS 15943, ZM 241385 and SCH 58261 as antagonists at human adenosine receptors
46. 2a receptors
47. Lipid polymorphism and protein-lipid interactions
48. Lipid composition and the lateral pressure profile in bilayers
49. Agonist-specific alterations in receptor binding affinity associated with solubilization of turkey erythrocyte membrane beta adrenergic receptors
50. 1-adrenergic receptors
51. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 percent inhibition (I50) of an enzymatic reaction