A robust and rapid method of producing soluble, stable, and functional g-protein coupled receptors

PLoS ONE

Volumn 6, Issue 10, 2011, Pages

  Corin, Karolina ^a   Baaske, Philipp ^b   Ravel, Deepali B ^a   Song, Junyao ^a   Brown, Emily ^a   Wang, Xiaoqiang ^a,c   Geissler, Sandra ^b   Wienken, Christoph J ^d   Jerabek Willemsen, Moran ^b   Duhr, Stefan ^b   Braun, Dieter ^d   Zhang, Shuguang ^a  

^a USA   (United States)

^b NanoTemper Technologies GmbH   (Germany)

^c CHINA UNIVERSITY OF PETROLEUM   (China)

^d UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

G PROTEIN COUPLED RECEPTOR; LIGAND;

ANALYTIC METHOD; ANTIBODY AFFINITY; ARTICLE; CELL FREE SYSTEM; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; LIGAND BINDING; MICROSCALE THERMOPHORESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN SYNTHESIS; QUANTITATIVE STUDY; STRUCTURE ACTIVITY RELATION; CELL LINE; CHEMISTRY; HUMAN; METABOLISM; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; SOLUBILITY;

CELL LINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; LIGANDS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, G-PROTEIN-COUPLED; SOLUBILITY;

EID: 80055040011     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023036     Document Type: Article

References (28)

1. Klammt C, Löhr F, Schäfer B, Haase W, Dötsch V, et al. (2004) High level cell-free expression and specific labeling of integral membrane proteins. Eur J Biochem 271: 568-580.
2. Klammt C, Schwarz D, Fendler K, Haase W, Dotsch V, et al. (2005) Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J 272: 6024-6038.
3. Klammt C, Schwarz D, Eifler N, Engel A, Piehler J, et al. (2007) Cell-free production of G protein-coupled receptors for functional and structural studies. J Struct Biol 158: 482-493.
4. Klammt C, Srivastava A, Eifler N, Junge F, Beyermann M, et al. (2007) Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation. FEBS J 274: 3257-3269.
5. Schwarz D, Junge F, Durst F, Frölich N, Schneider B, et al. (2007) Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. Nat Protoc 2: 2945-2957.
6. Schwarz D, Dötsch V, Bernhard F, (2008) Production of membrane proteins using cell-free expression systems. Proteomics 8: 3933-3946.
7. Junge F, Luh LM, Proverbio D, Schäfer B, Abele R, et al. (2010) Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptor. J. Struct. Biol 172: 94-106.
8. Ishihara G, Goto M, Saeki M, Ito K, Hori T, et al. (2005) Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors. Protein Expr Purif 41: 27-37.
9. Liguori L, Marques B, Lenormand JL, (2008) A bacterial cell-free expression system to produce membrane proteins and proteoliposomes: from cDNA to functional assay. Curr Protoc Protein Sci Chapter 5: Unit 5.22.
10. Kamonchanok S, Balog CI, van der Does AM, Booth R, de Grip WJ, et al. (2008) GPCR proteomics: mass spectrometric and functional analysis of histamine H1 receptor after baculovirus-driven and in vitro cell free expression. J Proteome Res 7: 621-629.
11. Maslennikov I, Klammt C, Hwang E, Kefala G, Okamura M, et al. (2010) Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis. Proc Natl Acad Sci U S A 107: 10902-10907.
12. Kai L, Kaldenhoff R, Lian J, Zhu X, Dötsch V, et al. (2010) Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes. PLoS ONE 5: e12972.
13. Savage DF, Anderson CL, Robles-Colmenares Y, Newby ZE, Stroud RM, (2007) Cell-free complements in vivo expression of the E. coli membrane proteome. Protein Sci 16: 966-976.
14. Kaiser L, Graveland-Bikker J, Steuerwald D, Vanberghem M, Herlihy K, et al. (2008) Large-scale production and study of a synthetic G protein-coupled receptor: human olfactory receptor 17-4. Proc Natl Acad Sci USA 105: 15726-15731.
15. Deniaud A, Liguori L, Blesneac I, Lenormand JL, Pebay-Peyroula E, (2008) Crystallization of the membrane protein hVDAC1 produced in cell-free system. Biochim Biophys Acta 1798: 1540-1546.
16. Wang X, Corin K, Baaske P, Wienken CJ, Jerabek-Willemsen M, et al. (2011) Designer lipid-like peptide surfactants for cell-free production of diverse functional G-protein coupled receptors. Proc. Natl. Acad. Sci. USA 118: 9049-9054.
17. Cook B, Steuerwald D, Kaiser L, Graveland-Bikker J, Vanberghem M, et al. (2009) Large scale production and study of a synthetic G-protein coupled receptor: Human olfactory receptor 17-4. Proc. Natl. Acad. Sci. USA 106: 11925-11930.
18. Cook BL, Ernberg KE, Chung H, Zhang S, (2008) Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line. PLoS ONE 3: e2920.
19. Ren H, Yu D, Ge B, Cook BL, Xu Z, et al. (2009) High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1. PLoS ONE 4: e4509.
20. Leck K-J, Zhang S, Hauser CAE, (2010) Study of bioengineered zebra fish olfactory receptor 131-2: receptor purification and secondary structure analysis. PLoS ONE 5: e15027.
21. Duhr S, Braun D, (2009) Why molecules move along a temperature gradient. Proc Natl Acad Sci U S A 103: 19678-19682.
22. Baaske P, Wienken CJ, Reineck P, Duhr S, Braun D, (2010) Optical thermophoresis for quantifying the buffer dependence of aptamer binding. Angew Chem Int Ed Engl 49: 2238-2241.
23. Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S, (2010) Protein-binding assays in biological liquids using microscale thermophoresis. Nature Communications 1: 100.
24. Shirokova E, Raguse JD, Meyerhof W, Krautwurst D, (2008) The human vomeronasal type-1 receptor family-detection of volatiles and cAMP signaling in HeLa/Olf cells. FASEB J. 22: 1416-1425.
25. Bass RB, Strop P, Barclay M, Rees DC, (2002) Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science 298: 1582-1587.
26. Wang W, Black SS, Edwards MD, Miller S, Morrison EL, et al. (2008) The structure of an open form of an E. coli mechanosensitive channel at 3.45 Å resolution. Science 321: 1179-1183.
27. Rasband WS, (1997-2011) ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA,http://imagej.nih.gov/ij/.
28. Abramoff MD, Magelhaes PJ, Ram SJ, (2004) Image processing with ImageJ. Biophotonics International 11 (7): 36-42.