A robust and rapid method of producing soluble, stable, and functional g-protein coupled receptors

PLoS ONE

Volumn 6, Issue 10, 2011, Pages

  Corin, Karolina ^a   Baaske, Philipp ^b   Ravel, Deepali B ^a   Song, Junyao ^a   Brown, Emily ^a   Wang, Xiaoqiang ^a,c   Geissler, Sandra ^b   Wienken, Christoph J ^d   Jerabek Willemsen, Moran ^b   Duhr, Stefan ^b   Braun, Dieter ^d   Zhang, Shuguang ^a  

^a Massachusetts Institute of Technology   (United States)

^b NanoTemper Technologies GmbH   (Germany)

^c CHINA UNIVERSITY OF PETROLEUM   (China)

^d UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

G PROTEIN COUPLED RECEPTOR; LIGAND;

ANALYTIC METHOD; ANTIBODY AFFINITY; ARTICLE; CELL FREE SYSTEM; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; LIGAND BINDING; MICROSCALE THERMOPHORESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN SYNTHESIS; QUANTITATIVE STUDY; STRUCTURE ACTIVITY RELATION; CELL LINE; CHEMISTRY; HUMAN; METABOLISM; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; SOLUBILITY;

CELL LINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; LIGANDS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, G-PROTEIN-COUPLED; SOLUBILITY;

EID: 80055040011     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023036     Document Type: Article

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