Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors

Biological Chemistry

Volumn 389, Issue 1, 2008, Pages 69-78

  Roy, Ankita ^a   Shukla, Arun Kumar ^a   Haase, Winfried ^a   Michel, Hartmut ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

Affinity purification; Cell morphology; G protein coupled receptors; Radioligand binding; Recombinant membrane proteins; Rhodobacter sphaeroides

Indexed keywords

ADENOSINE A2A RECEPTOR; ANGIOTENSIN 1A RECEPTOR; BRADYKININ B2 RECEPTOR; G PROTEIN COUPLED RECEPTOR;

ARTICLE; BACTERIAL MEMBRANE; CELL SURFACE; CONTROLLED STUDY; HOST; NONHUMAN; PHOTOTROPHIC BACTERIUM; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RECEPTOR SOLUBILIZATION; RHODOBACTER SPHAEROIDES;

CELL MEMBRANE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; HUMANS; INDICATORS AND REAGENTS; MICROSCOPY, ELECTRON; PHOTOSYNTHESIS; RADIOLIGAND ASSAY; RECEPTOR, ADENOSINE A2A; RECEPTOR, ANGIOTENSIN, TYPE 1; RECEPTOR, BRADYKININ B2; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT PROTEINS; RHODOBACTER SPHAEROIDES;

BACTERIA (MICROORGANISMS); PHOTOBACTERIA; RHODOBACTER SPHAEROIDES;

EID: 37349104746     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2008.001     Document Type: Article

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