A carboxy-terminal affinity tag for the purification and mass spectrometric characterization of integral membrane proteins

Journal of Proteome Research

Volumn 8, Issue 5, 2009, Pages 2388-2396

  Wong, Julie P ^a   Reboul, Emmanuelle ^a   Molday, Robert S ^a   Kast, Juergen ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

1D4 affinity tag; Annotated spectrum libraries; ATP binding cassette transporters; G protein coupled receptors; Membrane protein enrichment; Proteotypic peptides; Tandem mass spectrometry

Indexed keywords

ABC TRANSPORTER; ABC TRANSPORTER A1; ABC TRANSPORTER A4; CHEMOKINE RECEPTOR CCR5; CHEMOKINE RECEPTOR CXCR4; G PROTEIN COUPLED RECEPTOR; LIPOSOME; MONOCLONAL ANTIBODY; RHODOPSIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CELL LINE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; GLYCOSYLATION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEOMICS;

AMINO ACID SEQUENCE; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; CATTLE; CELL LINE; CERCOPITHECUS AETHIOPS; CHROMATOGRAPHY, LIQUID; COS CELLS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MASS SPECTROMETRY; MEMBRANE PROTEINS; MICROSCOPY, CONFOCAL; OLIGOPEPTIDES; RECEPTORS, CCR5; RECEPTORS, CXCR4; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; RHODOPSIN; TRANSFECTION;

BOVINAE;

EID: 66749153179     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr801008c     Document Type: Article

References (22)

1. Luche, S.; Santoni, V.; Rabilloud, T. Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two- dimensional electrophoresis. Proteomics 2003, 3 , 249-253.
2. Logsdon, C. D. The Influence of the cellular context on receptor function: a necessary consideration for physiologic interpretations of receptor expression studies. Life Sci. 1999, 64 (6/7), 369-374.
3. Molloy, M. P.; Herbert, B. R.; Williams, K. L.; Gooley, A. A. Extraction of Escerichia coli proteins with organic solvents prior to two-dimensional electrophoresis. Electrophoresis 1999, 20 , 701-704.
4. Terpe, K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 2003, 60 , 523-533.
5. Wu, C. C.; Yates, J. R., III. The application of mass spectrometry to membrane protomics. Nat. Biotechnol. 2003, 21 , 262-267.
6. Mirzaei, H.; McBee, J. K.; Watts, J.; Aebersold, R. Comparative evaluation of current peptide production platforms of current peptide production platforms used in absolute quantification in proteomics. Mol. Cell. Proteomics 2008, 7 (4), 813-823.
7. Mallick, P.; Schirle, M.; Chen, S. S.; Flory, M. R.; Lee, H.; Martin, D.; Ranish, J.; Raught, B.; Schmitt, R.; Werner, T.; Kuster, B.; Aebersold, R. Computational prediction of proteotypic peptides for quantitative proteomics. Nat. Biotechnol. 2007, 25 , 125-131.
8. Kuster, B.; Schirle, M.; Mallick, P.; Aebersold, R. Scoring proteome with proteotypic peptide probes. Nat. Rev. Mol. Cell Biol. 2005, 6 , 577583.
9. Craig, R.; Cortens, J. C.; Beavis, R. C. The use of proteotypic peptide libraries for protein identification. Rapid Commun. Mass Spectrom. 2005, 19 , 1844-1850.
10. MacKenzie, D.; Arendt, A.; Hargrave, P.; McDowell, J. H.; Molday, R. S. Localization of binding sites for carboxyl terminal specific anti-rhodopsin monoclonal antibodies using synthetic peptides. Biochemistry 1984, 23 (26), 6544-6549.
11. Hodges, R. S.; Heaton, R. J.; Parler, J. M.; Molday, L.; Molday, R. S. Antigen-antibody interaction. J. Biol. Chem. 1988, 263 , 11768-11775.
12. Ahn, J.; Beharry, S.; Molday, L. L.; Molday, R. S. Functional Interactions between the two halves of the photoreceptor-specific ATP binding cassette protein ABCR (ABCA4). J. Biol. Chem. 2003, 278 , 39600-39607.
13. Weber, B. H.; Schrewe, H.; Molday, L. L.; Gehrig, A.; White, K. L.; Seeliger, M. W.; Jaissle, G. B.; Friedburg, C.; Tamm, E.; Molday, R. S. Inactivation of the murine X-linked juvenile retinoschisis gene, Rs1h, suggests a role of retinoschisin in retinal cell layer organization and synaptic structure. Proc. Natl, Acad. Sci. U.S.A. 2002, 99 , 6222-6227.
14. Ahn, J.; Molday, R. S. Purification and characterization of ABCR from bovine rod outer segments. Methods Enzymol. 2000, 315 , 864-879.
15. Illing, M.; Molday, L. L.; Molday, R. S. The 220-kDa rim protein of retinal rod outer segments is a member of the ABC transporter superfamily. J.Biol.Chem. 1997, 272 (15), 10303-10310.
16. Takayama, H.; Chelikani, P.; Reeves, P. J.; Zhang, S.; Khorana, H. G. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 2008, 3 (6), e2314.
17. Chabot, D. J.; Chen, H.; Dimitrov, D. S.; Broder, C. C. N-linked glycosylation of CXCR4 masks coreceptor function for CCR5- dependent human immunodeficiency virus type 1 isolates. J. Virol. 2000, 74 (9), 4404-4413.
18. Wang, J.; Babcock, G. J.; Choe, H.; Farzan, M.; Sodroski, J.; Gabuzda, D. N-linked glycosylation in the CXCR4-N-terminus inhibits binding to HIV-1 envelope glycoproteins. Virology 2004, 324 (1), 140-150.
19. Kuster, B.; Schirle, M.; Mallick, P.; Aebersold, R. Scoring proteomes with proteotypic peptide probes. Nat.Rev. Mol. Cell Biol. 2005, 6 , 577-583.
20. Lange, V.; Malmstrom, J. A.; Didion, J.; King, N. L.; Johansson, B. P.; Schafer, J.; Rameseder, J.; Wong, C. H.; Deutsch, E. W.; Brusniak, M. Y.; Buhlmann, P.; Bjorck, L.; Domon, B.; Aerbersold, R. Targeted Quantitative Analysis of Streptococcus pyogenes Virulence Factors by Multiple Reaction Monitoring. Mol. Cell. Proteomics 2008, 7 (8), 1489-1500.
21. Lanctot, P. M.; Leclerc, P. C.; Clement, M.; Auger-Messier, M.; Escher, E.; Leduc, R.; Guillemette, G. Importance of N-glycosylation positioning for cell-surface expression, targeting, affinity and quality control of the human AT1 receptor. Biochem. J. 2005, 390 (1), 367-376.
22. Huskens, D.; Princen, K.; Schreiber, M.; Schols, D. The role of N-glycosylation sites on the CXCR4 receptor for CXCL-12 binding and signaling and X4 HIV-1 viral infectivity. Virology 2007, 363 , 280-287.