A cleavable ligand column for the rapid isolation of large quantities of homogeneous and functional neurotensin receptor 1 variants from E. coli

Protein Expression and Purification

Volumn 108, Issue , 2015, Pages 106-114

  Egloff, Pascal ^a   Deluigi, Mattia ^a   Heine, Philipp ^a   Balada, Stefanie ^a   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Crystal structure; Directed evolution; G protein coupled receptor; Ligand affinity purification; Membrane protein; Protein stability

Indexed keywords

NEUROTENSIN RECEPTOR; NEUROTENSIN TYPE 1 RECEPTOR; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; HUMANS; RECEPTORS, NEUROTENSIN; RECOMBINANT PROTEINS;

EID: 84925490966     PISSN: 10465928     EISSN: 10960279     Source Type: Journal    
DOI: 10.1016/j.pep.2014.10.006     Document Type: Article

References (33)

1. N. Vita, P. Laurent, S. Lefort, P. Chalon, X. Dumont, M. Kaghad, D. Gully, G. Le Fur, P. Ferrara, and D. Caput Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor FEBS Lett. 317 1993 139 142
2. G. Bissette, C.B. Nemeroff, P.T. Loosen, A.J. Prange Jr., and M.A. Lipton Hypothermia and intolerance to cold induced by intracisternal administration of the hypothalamic peptide neurotensin Nature 262 1976 607 609
3. R.M. Schimpff, C. Avard, G. Fenelon, A.M. Lhiaubet, L. Tenneze, M. Vidailhet, and W. Rostene Increased plasma neurotensin concentrations in patients with Parkinson's disease J. Neurol. Neurosurg. Psychiatry 70 2001 784 786
4. M. Alifano, F. Souaze, S. Dupouy, S. Camilleri-Broet, M. Younes, S.M. Ahmed-Zaid, T. Takahashi, A. Cancellieri, S. Damiani, M. Boaron, P. Broet, L.D. Miller, C. Gespach, J.F. Regnard, and P. Forgez Neurotensin receptor 1 determines the outcome of non-small cell lung cancer Clin. Cancer Res. 16 2010 4401 4410
5. G. Griebel, and F. Holsboer Neuropeptide receptor ligands as drugs for psychiatric diseases: the end of the beginning? Nat. Rev. Drug Discov. 11 2012 462 478
6. D. Skrzydelski, A.M. Lhiaubet, A. Lebeau, P. Forgez, M. Yamada, E. Hermans, W. Rostene, and D. Pelaprat Differential involvement of intracellular domains of the rat NTS1 neurotensin receptor in coupling to G proteins: a molecular basis for agonist-directed trafficking of receptor stimulus Mol. Pharmacol. 64 2003 421 429
7. K. Tanaka, M. Masu, and S. Nakanishi Structure and functional expression of the cloned rat neurotensin receptor Neuron 4 1990 847 854
8. P.R. Dobner Multitasking with neurotensin in the central nervous system Cell. Mol. Life Sci. 62 2005 1946 1963
9. D. Pelaprat Interactions between neurotensin receptors and G proteins Peptides 27 2006 2476 2487
10. J.I. Hwang, D.K. Kim, H.B. Kwon, H. Vaudry, and J.Y. Seong Phylogenetic history, pharmacological features, and signal transduction of neurotensin receptors in vertebrates Ann. N. Y. Acad. Sci. 1163 2009 169 178
11. J.F. White, N. Noinaj, Y. Shibata, J. Love, B. Kloss, F. Xu, J. Gvozdenovic-Jeremic, P. Shah, J. Shiloach, C.G. Tate, and R. Grisshammer Structure of the agonist-bound neurotensin receptor Nature 490 2012 508 513
12. C.A. Sarkar, I. Dodevski, M. Kenig, S. Dudli, A. Mohr, E. Hermans, and A. Plückthun Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity Proc. Natl. Acad. Sci. U.S.A. 105 2008 14808 14813
13. I. Dodevski, and A. Plückthun Evolution of three human GPCRs for higher expression and stability J. Mol. Biol. 408 2011 599 615
14. K.M. Schlinkmann, A. Honegger, E. Tureci, K.E. Robison, D. Lipovsek, and A. Plückthun Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations Proc. Natl. Acad. Sci. U.S.A. 109 2012 9810 9815
15. K.M. Schlinkmann, M. Hillenbrand, A. Rittner, M. Kunz, R. Strohner, and A. Plückthun Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution J. Mol. Biol. 422 2012 414 428
16. P. Egloff, M. Hillenbrand, C. Klenk, A. Batyuk, P. Heine, S. Balada, K.M. Schlinkmann, D.J. Scott, M. Schütz, and A. Plückthun Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli Proc. Natl. Acad. Sci. U.S.A. 111 2014 655 662
17. R.A. Venters, B.T. Farmer, C.A. Fierke, and L.D. Spicer Characterizing the use of perdeuteration in NMR studies of large proteins C-13, N-15 and H-1 assignments of human carbonic anhydrase II J. Mol. Biol. 264 1996 1101 1116
18. J.F. White, L.B. Trinh, J. Shiloach, and R. Grisshammer Automated large-scale purification of a G protein-coupled receptor for neurotensin FEBS Lett. 564 2004 289 293
19. J.F. White, and R. Grisshammer Automated large-scale purification of a recombinant G-protein-coupled neurotensin receptor Curr. Protoc. Protein Sci. 2007 Chapter 6, Unit 6 8
20. R. Grisshammer, J.F. White, L.B. Trinh, and J. Shiloach Large-scale expression and purification of a G-protein-coupled receptor for structure determination - an overview J. Struct. Funct. Genomics 6 2005 159 163
21. J. Tucker, and R. Grisshammer Purification of a rat neurotensin receptor expressed in Escherichia coli Biochem. J. 317 1996 891 899
22. A.M. Ring, A. Manglik, A.C. Kruse, M.D. Enos, W.I. Weis, K.C. Garcia, and B.K. Kobilka Adrenaline-activated structure of beta2-adrenoceptor stabilized by an engineered nanobody Nature 502 2013 575 579
23. T. Ohtaki, K. Ogi, Y. Masuda, K. Mitsuoka, Y. Fujiyoshi, C. Kitada, H. Sawada, H. Onda, and M. Fujino Expression, purification, and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide. large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells J. Biol. Chem. 273 1998 15464 15473
24. H.M. Weiss, and R. Grisshammer Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli Eur. J. Biochem. 269 2002 82 92
25. T. Warne, J. Chirnside, and G.F. Schertler Expression and purification of truncated, non-glycosylated turkey beta-adrenergic receptors for crystallization Biochim. Biophys. Acta 1610 2003 133 140
26. J.F. White, and R. Grisshammer Stability of the neurotensin receptor NTS1 free in detergent solution and immobilized to affinity resin PLoS One 5 2010
27. L. Nieba, S.E. Nieba-Axmann, A. Persson, M. Hamalainen, F. Edebratt, A. Hansson, J. Lidholm, K. Magnusson, A.F. Karlsson, and A. Plückthun BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip Anal. Biochem. 252 1997 217 228
28. P. Forrer, and R. Jaussi High-level expression of soluble heterologous proteins in the cytoplasm of Escherichia coli by fusion to the bacteriophage lambda head protein D Gene 224 1998 45 52
29. D.J. Scott, and A. Plückthun Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells J. Mol. Biol. 425 2013 662 677
30. W. Rostene, M. Azzi, H. Boudin, I. Lepee, F. Souaze, M. Mendez-Ubach, C. Betancur, and D. Gully Use of nonpeptide antagonists to explore the physiological roles of neurotensin. Focus on brain neurotensin/dopamine interactions Ann. N. Y. Acad. Sci. 814 1997 125 141
31. T.W. Moody Peptide hormones and lung cancer Panminerva Med. 48 2006 19 26
32. Y. Okuno, J. Yang, K. Taneishi, H. Yabuuchi, and G. Tsujimoto GLIDA: GPCR-ligand database for chemical genomic drug discovery Nucleic Acids Res. 34 2006 673 677
33. C. Reichen, S. Hansen, and A. Plückthun Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins J. Struct. Biol. 185 2013 147 162