An overview of enzymatic reagents for the removal of affinity tags

Protein Expression and Purification

Volumn 80, Issue 2, 2011, Pages 283-293

  Waugh, David S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

3C protease; Affinity tag; Aminopeptidase; Carboxypeptidase; Enterokinase; Enteropeptidase; Factor Xa; His tag; Polyhistidine tag; PreScission protease; TEV protease; Thrombin

Indexed keywords

EXOPEPTIDASE; PROTEINASE; RECOMBINANT PROTEIN;

AFFINITY LABELING; ANIMAL; CELL LINE; CHEMISTRY; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; METABOLISM; REVIEW; RHINOVIRUS; SOLUBILITY; TEMPERATURE;

AFFINITY LABELS; ANIMALS; CATALYTIC DOMAIN; CELL LINE; ENDOPEPTIDASES; ENZYME ACTIVATION; ESCHERICHIA COLI; EXOPEPTIDASES; GENETIC VECTORS; RECOMBINANT PROTEINS; RHINOVIRUS; SOLUBILITY; SUBSTRATE SPECIFICITY; TEMPERATURE;

TOBACCO ETCH VIRUS;

EID: 80054052318     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.08.005     Document Type: Review

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