GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

Nature Protocols

Volumn 3, Issue 5, 2008, Pages 784-798

  Drew, David ^a   Newstead, Simon ^a   Sonoda, Yo ^a,b   Kim, Hyun ^c   von Heijne, Gunnar ^c   Iwata, So ^a,d  

^a Membrane Protein Crystallography Group   (United Kingdom)

^b KAKEN PHARMACEUTICAL CO LTD   (Japan)

^c STOCKHOLM UNIVERSITY   (Sweden)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN;

ARTICLE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; EUKARYOTE; FLUORESCENCE ANALYSIS; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; HETEROLOGOUS EXPRESSION; HOMOLOGOUS RECOMBINATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; STRUCTURAL GENOMICS;

BIOTECHNOLOGY; CHROMATOGRAPHY, GEL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 43149098999     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.44     Document Type: Article

References (28)

1. Drew, D.E., von Heijne, G., Nordlund, P. & de Gier, J.W. Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett. 507, 220-224 (2001).
2. Drew, D., Lerch, M., Kunji, E., Slotboom, D.J. & de Gier, J.W. Optimization of membrane protein overexpression and purification using GFP fusions. Nat. Methods 3, 303-313 (2006).
3. Drew, D. et al. A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci. 14, 2011-2017 (2005).
4. Kawate, T. & Gouaux, E. Fluorescence-detection size-exctusion chromatography for precrystallization screening of integral membrane proteins. Structure 14, 673-681 (2006).
5. Jasti, J., Furukawa, H., Gonzales, E.B. & Gouaux, E. Structure of acid-sensing ion channel 1 at 1.9 A resolution and Low pH. Nature 449, 316-323 (2007).
6. Newstead, S., Kim, H., von Heijne, G., Iwata, S. & Drew, D. High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharornyces cerevisiae. Proc. Natl. Acad. Sci. USA 104, 13936-13941 (2007).
7. Ferguson, A.D. et al Crystal structure of inhibitor-bound human 5-lipoxygenase-activating protein. Science 317, 510-512 (2007).
8. Nyblom, M. et al. Exceptional overproduction of a functional human membrane protein. Protein Expr. Purif. 56, 110-120 (2007).
9. Jidenko, M. et al. Crystallization of a mammalian membrane protein overexpressed in Saccharomyces cerevisicre. Proc. Natl. Acad. Sci. USA 102, 11687-11691 (2005).
10. Pedersen, B.P., Buch-Pedersen, M.J., Morth, J.P., Palmgren, M.G. & Nissen, P. Crystal structure of the plasma membrane proton pump. Nature 450, 1111-1114 (2007).
11. Ellgaard, L. & Helenius, A. Quality control in the encloptasmic reticulum. Nat. Rev. Mol. Cell. Biol. 4, 181-191 (2003).
12. Nakatsukasa, K., Huyer, G., Michaelis, S. & Brodsky, J.L. Dissecting the ER-associated degradation of a misfolded polytopic membrane protein. Cell 132, 101-112. (2008).
13. Niebauer, R.T. & Robinson, A.S. Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae. Protein Expr. Purif. 46, 204-211 (2006).
14. Kota, J., Gilstring, C.F. & Ljungdahl, P.O. Membrane chaperone Shr3 assists in folding amino acid permeases preventing precocious ERAD. J. Cell Biol. 176, 617-628 (2007).
15. Cormack, B.P. et al. Yeast-enhanced green fluorescent protein (yEGFP)a reporter ofgene expression in Candida albicans. Microbiology 143 (Pt 2), 303-311 (1997).
16. Lucast, L.J., Batey, R.T. & Doudna, J.A. Large-scale purification of a stable form of recombinant tobacco etch virus protease. Biotechniques 30, 544-546, 548, 550 passim (2001).
17. Mumberg, D., Müller, R. & Funk, M. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156, 119-122 (1995).
18. Sato, K., Sato, M. & Nakano, A. Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamicaity localized to the Golgi apparatus by coatomer. J. Cell Biol., 152, 935-944 (2001).
19. Wagner, S., Bader, M.L., Drew, D. & de Gier, J.W. Rationalizing membrane protein overexpression. Trends Biotechnol. 24, 364-371 (2006).
20. Andre, N. et al. Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via single expression screen. Protein Sci. 15, 1115-1126 (2006).
21. Drew, D. et al. Rapid topology mapping of Escheichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc. Natl. Acad. Sci. USA 99, 2690-2695 (2902).
22. Huh, W.K. et al. GlobaL analysis of protein localization in budding yeast. Nature 425, 686-691 (2003).
23. Guan, L., Mirza, O., Verner, G., Iwata, S. & Kaback, H.R. Structural determination of wild-type lactose permease. Proc. Natl. Acad. Sci. USA 104, 15294-15298 (2007).
24. Long, S.B., Tao, X., Campbell, E.B. & MacKinnon, R. Atomic structure of a voltage-dependent K channel in a lipid membrane-like environment. Nature 450, 376-382 (2007).
25. Burke, D., Dawson, D. Stearns, T. Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, 2000 edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2000).
26. Stimpson, H.E., Lewis, M.J. & Pelham, H.R. Transferrin receptor-like proteins control the degradation of a yeast metal transporter. EMBO J. 25, 662-672 (2006).
27. Galan, J.M., Moreau, V., Andre, B., Volland, C. & Haguenauer-Tsapis, R. Ubiquitination mediated by the Npi1p/Rsp5p ubiquitin-protein ligase is required for endocytosis of the yeast uracil permease. J Biol. Chem. 271, 10946-10952 (1996).
28. Liu, J., Sitaram, A. & Burd, C.G. Regulation of copper-dependent endocytosis and vacuolar degradation of the yeast copper transporter, Ctr1p, by the Rsp5 ubiquitin ligase. Traffic 8, 1375-1384 (2007).