Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris

Biochemical and Biophysical Research Communications

Volumn 380, Issue 2, 2009, Pages 271-276

  Yurugi Kobayashi, Takami ^a,b   Asada, Hidetsugu ^a   Shiroishi, Mitsunori ^a,b   Shimamura, Tatsuro ^a,b   Funamoto, Saeko ^a   Katsuta, Naoko ^a   Ito, Keisuke ^c   Sugawara, Taishi ^c   Tokuda, Natsuko ^b   Tsujimoto, Hirokazu ^a   Murata, Takeshi ^a,b   Nomura, Norimichi ^a   Haga, Kazuko ^d   Haga, Tatsuya ^d   Iwata, So ^a,b,e   Kobayashi, Takuya ^a,b  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d GAKUSHUIN UNIVERSITY   (Japan)

^e IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

G protein coupled receptor (GPCR); Membrane protein; Non glycosylation; Pichia pastoris; Structural biology

Indexed keywords

G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; MUSCARINIC M2 RECEPTOR;

AMINO ACID SEQUENCE; ARTICLE; COMPARATIVE STUDY; CRYSTALLIZATION; GLYCOSYLATION; METHYLOTROPHIC YEAST; NONHUMAN; PH; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; TEMPERATURE;

GLYCOSYLATION; HUMANS; PICHIA; PROTEIN BIOSYNTHESIS; RECEPTOR, MUSCARINIC M2; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 60349124153     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.01.053     Document Type: Article

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