Optimization of protein production in mammalian cells with a coexpressed fluorescent marker

Structure

Volumn 12, Issue 8, 2004, Pages 1355-1360

  Mancia, Filippo ^a   Patel, Saurabh D ^a   Rajala, Michael W ^b   Scherer, Philipp E ^b   Nemes, Adriana ^a   Schieren, Ira ^a   Hendrickson, Wayne A ^a   Shapiro, Lawrence ^a,c  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^c New York Presbyterian Hospital   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; MOLECULAR MARKER; RESISTIN; SEROTONIN RECEPTOR;

ARTICLE; CELL LINE; CHEMICAL PROCEDURES; CRYSTAL STRUCTURE; CULTURE MEDIUM; EXPRESSION VECTOR; GENETIC TRANSFECTION; HYPOTHESIS; INTERNAL RIBOSOME ENTRY SITE; MAMMAL CELL; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

BACTERIA (MICROORGANISMS); MAMMALIA; VECTORS;

EID: 4143053500     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.str.2004.06.012     Document Type: Note

References (28)

1. Aruna B., Ghosh S., Singh A.K., Mande S.C., Srinivas V., Chauhan R., Ehtesham N.Z. Human recombinant resistin protein displays a tendency to aggregate by forming intermolecular disulfide linkages. Biochemistry. 42:2003;10554-10559
2. Backstrom J.R., Westphal R.S., Canton H., Sanders-Bush E. Identification of rat serotonin 5-HT2C receptors as glycoproteins containing N-linked oligosaccharides. Brain Res. Mol. Brain Res. 33:1995;311-318
3. Baneyx F. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10:1999;411-421
4. Chalfie M. Green fluorescent protein. Photochem. Photobiol. 62:1995;651-656
5. Galbraith D.W., Anderson M.T., Herzenberg L.A. Flow cytometric analysis and FACS sorting of cells based on GFP accumulation. Methods Cell Biol. 58:1999;315-341
6. Geisse S., Gram H., Kleuser B., Kocher H.P. Eukaryotic expression systems. a comparison Protein Expr. Purif. 8:1996;271-282
7. Grisshammer R., Tate C.G. Overexpression of integral membrane proteins for structural studies. Q. Rev. Biophys. 28:1995;315-422
8. Hendrickson W.A. Synchrotron crystallography. Trends Biochem. Sci. 25:2000;637-643
9. Holcomb I.N., Kabakoff R.C., Chan B., Baker T.W., Gurney A., Henzel W., Nelson C., Lowman H.B., Wright B.D., Skelton N.J., et al. FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family. EMBO J. 19:2000;4046-4055
10. Ibrahim S.F., van den Engh G. High-speed cell sorting. fundamentals and recent advances Curr. Opin. Biotechnol. 14:2003;5-12
11. Juan C.C., Kan L.S., Huang C.C., Chen S.S., Ho L.T., Au L.C. Production and characterization of bioactive recombinant resistin in Escherichia coli. J. Biotechnol. 103:2003;113-117
12. Julius D., MacDermott A.B., Axel R., Jessell T.M. Molecular characterization of a functional cDNA encoding the serotonin 1c receptor. Science. 241:1988;558-564
13. Liu X., Constantinescu S.N., Sun Y., Bogan J.S., Hirsch D., Weinberg R.A., Lodish H.F. Generation of mammalian cells stably expressing multiple genes at predetermined levels. Anal. Biochem. 280:2000;20-28
14. Makrides S.C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60:1996;512-538
15. Meng Y.G., Liang J., Wong W.L., Chisholm V. Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells. Gene. 242:2000;201-207
16. Mirzabekov T., Bannert N., Farzan M., Hofmann W., Kolchinsky P., Wu L., Wyatt R., Sodroski J. Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor. J. Biol. Chem. 274:1999;28745-28750
17. Palczewski K., Kumasaka T., Hori T., Behnke C.A., Motoshima H., Fox B.A., Le Trong I., Teller D.C., Okada T., Stenkamp R.E., et al. Crystal structure of rhodopsin. A G protein-coupled receptor Science. 289:2000;739-745
18. Patel S.D., Rajala M.W., Rossetti L., Scherer P.E., Shapiro L. Disulfide-dependent multimeric assembly of resistin family hormones. Science. 304:2004;1154-1158
19. Okada T., Fujiyoshi Y., Silow M., Navarro J., Landau E.M., Shichida Y. Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography. Proc. Natl. Acad. Sci. USA. 99:2002;5982-5987
20. Rajala M.W., Obici S., Scherer P.E., Rossetti L. Adipose-derived resistin and gut-derived resistin-like molecule-beta selectively impair insulin action on glucose production. J. Clin. Invest. 111:2003;225-230
21. Reeves P.J., Callewaert N., Contreras R., Khorana H.G. Structure and function in rhodopsin. high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N- acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line Proc. Natl. Acad. Sci. USA. 99:2002;13419-13424. a
22. Reeves P.J., Kim J.M., Khorana H.G. Structure and function in rhodopsin. a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants Proc. Natl. Acad. Sci. USA. 99:2002;13413-13418. b
23. Sarramegna V., Talmont F., Demange P., Milon A. Heterologous expression of G-rotein-coupled receptors. comparison of expression systems fron the standpoint of large-scale production and purification Cell. Mol. Life Sci. 60:2003;1529-1546
24. Steppan C.M., Bailey S.T., Bhat S., Brown E.J., Banerjee R.R., Wright C.M., Patel H.R., Ahima R.S., Lazar M.A. The hormone resistin links obesity to diabetes. Nature. 409:2001;307-312
25. Thomsen D.R., Stenberg R.M., Goins W.F., Stinski M.F. Promoter-regulatory region of the major immediate early gene of human cytomegalovirus. Proc. Natl. Acad. Sci. USA. 81:1984;659-663
26. Tsien R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67:1998;509-544
27. Vagner S., Galy B., Pyronnet S. Irresistible IRES. Attracting the translation machinery to internal ribosome entry sites. EMBO Rep. 2:2001;893-898
28. Wuthrich K. NMR Studies of Structure and Function of Biological Macromolecules (Nobel Lecture). Angew. Chem. Int. Ed. Engl. 42:2003;3340-3363