Cell-free production of G protein-coupled receptors for functional and structural studies

Journal of Structural Biology

Volumn 158, Issue 3, 2007, Pages 482-493

  Klammt, Christian ^a   Schwarz, Daniel ^a   Eifler, Nora ^b   Engel, Andreas ^b   Piehler, Jacob ^a   Haase, Winfried ^c   Hahn, Steffen ^a   Dötsch, Volker ^a   Bernhard, Frank ^a  

^a GOETHE UNIVERSITY   (Germany)

^b UNIVERSITY OF BASEL   (Switzerland)

^c MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

Corticotropin releasing factor receptor; Detergent; Endothelin B receptor; Melatonin 1B receptor; Neuropeptide Y4 receptor; Single particle analysis; TIRFS; Vasopressin type 2 receptor

Indexed keywords

CORTICOTROPIN; DETERGENT; ENDOTHELIN B RECEPTOR; G PROTEIN COUPLED RECEPTOR; MELATONIN 1 RECEPTOR; MELATONIN 1B RECEPTOR; MEMBRANE PROTEIN; NEUROPEPTIDE Y4 RECEPTOR; POLYOXYETHYLENE STEARYL ETHER; RHODOPSIN; UNCLASSIFIED DRUG; VASOPRESSIN RECEPTOR; VASOPRESSIN TYPE 2 RECEPTOR;

ARTICLE; BACTERIAL CELL; CELL FREE SYSTEM; CONTROLLED STUDY; ESCHERICHIA COLI; FREEZE FRACTURE; HUMAN; HYDROPHOBICITY; LIGAND BINDING; MICELLE; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; RAT;

ANIMALS; CELL EXTRACTS; CELL-FREE SYSTEM; DETERGENTS; ESCHERICHIA COLI; HUMANS; LIGANDS; LIPOSOMES; POLYETHYLENE GLYCOLS; RATS; RECEPTORS, G-PROTEIN-COUPLED; SOLUBILITY;

ESCHERICHIA COLI; EUKARYOTA; RATTUS; SUS;

EID: 34248572721     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2007.01.006     Document Type: Article

References (52)

1. Akermoun M., Koglin M., Zvalova-Iooss D., Folschweiller N., Dowell S.J., and Gearing K.L. Characterization of 16 human G protein-coupled receptors expressed in baculovirus-infected insect cells. Protein Expr. Purif. 44 (2005) 65-74
2. André N., Cherouati N., Prual C., Steffan T., Zeder-Lutz G., Magnin T., Pattui{dotless}̀ F., Michel H., Wagner R., and Reinhart C. Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci. 15 (2006) 1115-1126
3. Ayoub M.A., Coutourier C., Lucas-Meunier E., Angers S., Fossier P., Bouvier M., and Jockers R. Monitoring of ligand-independent dimerization and ligand-induced conformational changes of melatonin receptors in living cells by bioluminescence resonance energy transfer. J. Biol. Chem. 277 (2002) 21522-21528
4. Bai M. Dimerization of G-protein-coupled receptors: roles in signal transduction. Cell. Signal. 16 (2004) 175-186
5. Berrier C., Park K.H., Abes S., Bibonne A., Betton J.M., and Ghazi A. Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. Biochemistry 43 (2004) 12585-12591
6. Dinger M.C., Bader J.E., Kobor A.D., Kretschmar A.K., and Beck-Sickinger A.G. Homodimerization of neuropeptide y receptors investigated by fluorescence resonance energy transfer in living cells. J. Biol. Chem. 278 (2003) 10562-71051
7. Elbaz Y., Steiner-Mordoch S., Danieli T., and Schuldiner S. In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state. Proc. Natl. Acad. Sci. USA 101 (2004) 1519-1524
8. Elshourbagy N.A., Korman D.R., Wu H.L., Sylvester D.R., Lee J.A., Nuthalaganti P., Bergsma D.J., Kumar C.S., and Nambi P. Molecular characterization and regulation of the human endothelin receptors. J. Biol. Chem. 268 (1993) 3873-3879
9. Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A., and Palczewski K. Atomic-force microscopy: rhodopsin dimers in native disc membranes. Nature 421 (2003) 127-128
10. Fotiadis D., Jastrzebska B., Philippsen A., Müller D.J., Palczewski K., and Engel A. Structure of the rhodopsin dimer: a working model for G-protein-coupled receptors. Curr. Opin. Struct. Biol. 16 (2006) 252-259
11. Frank J., Verschoor A., and Boublik M. Multivariate statistical analysis of ribosome electron micrographs. L and R lateral views of the 40 S subunit from HeLa cells. J. Mol. Biol. 161 (1982) 107-133
12. Frank J., Radermacher M., Pencek P., Zhu J., Li Y., Ladjadj M., and Leith A. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J. Struct. Biol. 116 (1996) 190-199
13. Gavutis M., Lata S., Lamken P., Müller P., and Piehler J. Lateral ligand-receptor interactions on membranes probed by simultaneous fluorescence-interference detection. Biophys. J. 88 (2005) 4289-4302
14. Gregan B., Schaefer M., Rosenthal W., and Oksche A. Fluorescence resonance energy transfer analysis reveals the existence of endothelin-A and endothelin-B receptor homodimers. J. Cardiovasc. Pharmacol. 44 (2004) S30-S33
15. Grisshammer R., White J.F., Trinh L.B., and Shiloach J. Large-scale expression and purification of a G-protein-coupled receptor for structure determination-an overview. J. Struct. Funct. Genom. 6 (2005) 159-163
16. Hansen J.L., and Sheikh S.P. Functional consequences of 7TM receptor dimerization. Eur. J. Pharmaceut. Sci. 23 (2004) 301-317
17. Hay D.L., Poyner D.R., and Sexton P.M. GPCR modulation by RAMPs. Pharmacol. Ther. 109 (2006) 173-197
18. Hebert T.E., and Bouvier M. Structural and functional aspects of G protein-coupled receptor oligomerization. Biochem. Cell. Biol. 76 (1998) 1-10
19. Hiley C.R., Jones C.R., Pelton J.T., and Miller R.C. Binding of [125I]-endothelin-1 to rat cerebellar homogenates and its interactions with some analogues. Br. J. Pharmacol. 101 (1990) 319-324
20. Hulme E.C., and Curtis C.A. Purification of recombinant M1 muscarinic acetylcholine receptor. Biochem. Soc. Trans. 26 (1998) S361
21. Ishihara G., Goto M., Saeki M., Ito K., Hori T., Kigawa T., Shirouzu M., and Yokoyama S. Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors. Protein Expres. Purif. 41 (2005) 27-37
22. Kigawa T., Yabuki T., Yoshida Y., Tsutsui M., Ito Y., Shibata T., and Yokoyama S. Cell-free production and stable-isotope labeling of milligram quantities of proteins. FEBS Lett. 442 (1999) 15-19
23. Klabunde T., and Hessler G. Drug design strategies for targeting G-protein-coupled receptors. ChemBioChem 3 (2002) 928-944
24. Klammt C., Löhr F., Schafer B., Haase W., Dötsch V., Rüterjans H., Glaubitz C., and Bernhard F. High level cell-free expression and specific labeling of integral membrane proteins. Eur. J. Biochem. 271 (2004) 568-580
25. Klammt C., Schwarz D., Fendler K., Haase W., Dötsch V., and Bernhard F. Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel- type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J. 272 (2005) 6024-6038
26. Klammt C., Schwarz D., Löhr F., Schneider B., Dötsch V., and Bernhard F. Cell-free expression as an emerging technique for the large scale production of integral membrane proteins. FEBS J. 273 (2006) 4141-4153
27. Kraetke O., Wiesner B., Eichhorst J., Furkert J., Bienert M., and Beyermann M. Dimerization of corticotropin-releasing factor receptor type 1 is not coupled to ligand binding. J. Recept. Signal Transduct. Res. 25 (2005) 251-276
28. Kristiansen K. Molecular mechanisms of ligand binding, signalling, and regulation within the superfamily of G-protein-coupled receptors: molecular modelling and mutagenesis approaches to receptor structure and function. Pharmacol. Ther. 103 (2004) 21-80
29. Lata S., and Piehler J. Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush. Anal. Chem. 77 (2005) 1096-1105
30. De Leon H., and Garcia R. Characterization of endothelin receptor subtypes in isolated rat renal preglomerular microvessels. Regul. Pept. 60 (1995) 1-8
31. Li Y., Wang E., and Wang Y. A modified protocol for fast purification of T7 RNA polymerase. Protein Expr. Purif. 16 (1999) 355-358
32. Ludtke S.J., Baldwin P.R., and Chiu W. EMAN: semiautomated software for high- resolution single-particle reconstructions. J. Struct. Biol. 128 (1999) 82-97
33. Lundstrom, K., Wagner, R., Reinhart, C., Desmyter, A., Cherouati, N., Magnin, T., Zeder- Lutz, G., Courtot, M., Prual, C., Andre, N., Hassaine, G., Michel, H., Cambillau, C., Pattus, F., 2006. Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems. J. Struct. Funct. Genomics Nov 22; [Epub ahead of print, DOI 10.1007/s10969-006-9011-2].
34. Massotte D. G protein-coupled receptor overexpression with the baculovirus-insect cell system: a tool for structural and functional studies. Biochim. Biophys. Acta 1610 (2003) 77-89
35. 15N- labelled proteins for rapid analysis by NMR spectroscopy. Eur. J. Biochem. 271 (2004) 4084-4093
36. Penczek P., Radermacher M., and Frank J. Three-dimensional reconstruction of single particles embedded in ice. Ultramicroscopy 40 (1992) 33-53
37. Palczewski K., Kumasaka T., Hori T., Behnke C.A., Motoshima H., Fox B.A., LeTrong I., Teller D.C., Okada T., Stenkamp R.E., Yamamoto M., and Miyano M. Crystal structure of rhodopsin: a G protein-coupled receptor. Science 289 (2000) 739-745
38. Rached M., El Mourabit H., Buronfosse A., Blondet A., Naville D., Begeot M., and Penhoat A. Expression of the human melanocortin-2 receptor in different eukaryotic cells. Peptides 26 (2005) 1842-1847
39. Rios C.D., Jordan B.A., Gomes I., and Devi L.A. G-protein-coupled receptor dimerization: modulation of receptor function. Pharmacol. Ther. 92 (2001) 71-87
40. Sarramegna V., Demange P., Milon A., and Talmont F. Optimizing functional versus total expression of the human u-opiod receptor in Picchia pastoris. Protein Expr. Purif. 24 (2002) 212-220
41. Sarramegna V., Talmont F., Demange P., and Milon A. Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification. Cell. Mol. Life Sci. 60 (2003) 1529-1546
42. Schiller H., Haase W., Molsberger E., Janssen P., Michel H., and Reilander H. The human ET(B) endothelin receptor heterologously produced in the methylotrophic yeast Pichia pastoris shows high-affinity binding and induction of stacked membranes. Receptors Cannels 7 (2000) 93-107
43. Schwarz D., Klammt C., Koglin A., Löhr F., Schneider B., Dötsch V., and Bernhard F. Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies. Methods online (2006)
44. Spirin A.S., Baranov V.I., Ryabova L.A., Ovodov S.Y., and Alakhov Y.B. A continuous cell-free translation system capable of producing polypeptides in high yield. Science 242 (1988) 1162-1164
45. Tate C.G., and Blakely R.D. The effect of N-linked glycosylation on activity of the Na(+)- and Cl(-)-dependent serotonin transporter expressed using recombinant baculovirus in insect cells. J. Biol. Chem. 269 (1994) 26303
46. Tate C.G. Overexpression of mammalian integral membrane proteins for structural studies. FEBS Letts. 504 (2001) 94-98
47. Tate C.G., Haase J., Baker C., Boorsma M., Magnani F., Vallis Y., and Williams D.C. Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter. Biochim. Biophys. Acta 1610 (2003) 141-153
48. Torizawa T., Shimizu M., Taoka M., Miyano H., and Kainosho M. Efficient protocol of isotopically labelled proteins by cell-free synthesis: a practical protocol. J. Biomol. NMR 30 (2004) 311-325
49. Trbovic N., Klammt C., Koglin A., Löhr F., Bernhard F., and Dötsch V. Efficient strategy for the rapid backbone assignment of membrane proteins. J. Am. Chem. Soc. 127 (2005) 13504-13505
50. Tucker J., and Grisshammer R. Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem. J. 317 (1996) 891-899
51. Wagner S., Bader M.L., Drew D., and de Gier J.W. Rationalizing membrane protein overexpression. Trends Biotechnol. 24 (2006) 364-371
52. Zhu X., and Wess J. Truncated V2 vasopressin receptors as negative regulators of wild-type V2 receptor function. Biochemistry 37 (1998) 15773-15778