Efficient expression and immunoaffinity purification of human trace amine-associated receptor 5 from E. coli cell-free system

Protein and Peptide Letters

Volumn 20, Issue 4, 2013, Pages 473-480

  Wang, Xiaoqiang ^a   Cui, Ying ^b   Wang, Jiqian ^a  

^a CHINA UNIVERSITY OF PETROLEUM   (China)

^b Qingdao Technical College   (China)

Author keywords

Cell free system; Detergent; Efficient expression; G protein coupled receptor; Human trace amine associated receptor 5; Immunoaffinity purification

Indexed keywords

G PROTEIN COUPLED RECEPTOR; HUMAN TRACE AMINE ASSOCIATED RECEPTOR 5; HUMAN TRACE AMINE ASSOCIATED RECEPTOR 5 T4 LYSOZYME FUSION PROTEIN; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ALPHA HELIX; ANTIBODY AFFINITY; ARTICLE; CELL FREE SYSTEM; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTALLIZATION; DNA TEMPLATE; DOT HYBRIDIZATION; ESCHERICHIA COLI; GEL FILTRATION; MOLECULAR CLONING; NONHUMAN; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; WESTERN BLOTTING;

AMINO ACID SEQUENCE; CELL-FREE SYSTEM; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; DETERGENTS; ESCHERICHIA COLI; HUMANS; MOLECULAR SEQUENCE DATA; MURAMIDASE; POLYETHYLENE GLYCOLS; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS;

EID: 84877953104     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986613805290444     Document Type: Article

References (39)

1. Lindemann, L.; Hoener, M.C. A renaissance in trace amines inspired by a novel GPCR family. Trends Pharmacol. Sci., 2005, 26, 274-281.
2. Zucchi, R.; Chiellini, G.; Scanlan, T.S.; Grandy, D.K. Trace amineassociated receptors and their ligands. Br. J. Pharmacol., 2006, 149, 967-978.
3. Berry, M.D. The potential of trace amines and their receptors for treating neurological and psychiatric diseases. Rev. Recent Clin. Trials, 2007, 2, 3-19.
4. Branchek, T.A.; Blackburn, T.P. Trace amine receptors as targets for novel therapeutics: legend, myth and fact. Curr. Opin. Pharmacol., 2003, 3, 90-97.
5. Liberles, S.D.; Buck, L.B. A second class of chemosensory receptors in the olfactory epithelium. Nature, 2006, 442, 645-650.
6. Fleischer, J.; Breer, H.; Strotmann, J. Mammalian olfactory receptors. Front Cell Neurosci., 2009, 3, 1-10.
7. Borowsky, B.; Adham, N.; Jones, K.A.; Raddatz, R.; Artymyshyn, R.; Ogozalek, K.L.; Durkin, M.M.; Lakhlani, P.P.; Bonini, J.A.; Pathirana, S.; Boyle, N.; Pu, X.; Kouranova, E.; Lichtblau, H.; Ochoa, F.Y.; Branchek, T.A.; Gerald, C. Trace amines: identification of a family of mammalian G protein-coupled receptors. Proc. Natl. Acad. Sci. USA, 2001, 98, 8966-8971.
8. Lindemann, L.; Ebeling, M.; Kratochwil, N.A.; Bunzow, J.R.; Grandy, D.K.; Hoener, M.C. Trace amine-associated receptors form structurally and functionally distinct subfamilies of novel G protein-coupled receptors. Genomics, 2005, 85, 372-385.
9. Lewin, A.H. Receptors of Mammalian Trace Amines. AAPS J., 2006, 8, 138-145.
10. Lundstrom, K. Structural genomics of GPCRs. Trends Biotechnol., 2005, 23, 103-108.
11. Lundstrom, K. Latest development in drug discovery on G proteincoupled receptors. Curr. Protein Pept. Sci., 2006, 7, 465-470.
12. Krautwurst, D. Human olfactory receptor families and their odorants. Chem. Biodivers., 2008, 5, 842-852.
13. Gaillard, I.; Rouquier, S.; Giorgi, D. Olfactory receptors. Cell Mol. Life Sci., 2004, 61, 456-469.
14. Nelson, D.A.; Tolbert, M.D.; Singh, S.J.; Bost, K.L. Expression of neuronal trace amine-associated receptor (Taar) mRNAs in leukocytes. J. Neuroimmunol., 2007, 192, 21-30.
15. Rosenbaum, D.M.; Rasmussen, S.G.; Kobilka, B.K. The structure and function of G-protein-coupled receptors. Nature, 2009, 459, 356-363.
16. Walian, P.; Cross, T.A.; Jap, B.K. Structural genomics of membrane proteins. Genome Biol., 2004, 5, 215.
17. Lundstrom, K. Structural biology of G protein-coupled receptors. Bioorg. Med. Chem. Lett., 2005, 15, 3654-3657.
18. Zeng, Z.; Fan, P.; Rand, E.; Kyaw, H.; Su, K.; Madike, V.; Carter, K.C.; Li, Y. Cloning of a putative human neurotransmitter receptor expressed in skeletal muscle and brain, Biochem. Biophys. Res. Comm., 1998, 242, 575-578.
19. Cherezov, V.; Rosenbaum, D.M.; Hanson, M.A.; Rasmussen, S.G.; Thian, F.S.; Kobilka, T.S.; Choi, H.J.; Kuhn, P.; Weis, W.I.; Kobilka, B.K.; Stevens, R.C. High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. Science, 2007, 318, 1258-1265.
20. Rosenbaum, D.M.; Cherezov, V.; Hanson, M.A.; Rasmussen, S.G.; Thian, F.S.; Kobilka, T.S.; Choi, H.J.; Yao, X.J.; Weis, W.I.; Stevens, R.C.; Kobilka, B.K. GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function. Science, 2007, 318, 1266-1273.
21. Sarramegna, V.; Talmont, F.; Demange, P.; Milon, A. Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification, Cell Mol. Life Sci., 2003, 60, 1529-1546.
22. Sarramegn, V.; Muller, I.; Milon, A.; Talmont, F. Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure. Cell Mol. Life Sci., 2006, 63, 1149-1164.
23. Klammt, C.; Schwarz, D.; Fendler, K.; Haase, W.; Dtsch, V.; Bernhard, F. Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J., 2005, 272,6024-6038.
24. Klammt, C.; Schwarz, D.; Eifler, N.; Engel, A.; Piehler, J.; Haase, W.; Hahn, S.; Dtsch, V.; Bernhard, F. Cell-free production of G protein-coupled receptors for functional and structural studies. J. Struct. Biol., 2007, 158, 482-493.
25. Chen, Y.J.; Pornillos, O.; Lieu, S.; Ma, C.; Chen, A.P.; Chang, G. X-ray structure of EmrE supports dual topology model, Proc. Natl. Acad. Sci. USA, 2007, 104, 18999-19004.
26. Schneider, B.; Junge, F.; Shirokov, V.A.; Durst, F.; Schwarz, D.; Dtsch, V.; Bernhard, F. Membrane protein expression in cell-free systems, Methods Mol. Biol., 2010, 601, 165-186.
27. Wang, X.; Corin, K.; Baaske, P.; Wienken, C.J.; Jerabek- Willemsen, M.; Duhr, S.; Braun, D.; Zhang, S. Peptide surfactants for cell-free production of functional G protein-coupled receptors. Proc. Natl. Acad. Sci. USA, 2011, 108, 9049-9054.
28. Kaiser, L.; Graveland-Bikker, J.; Steuerwald, D.; Vanberghem, M.; Herlihy, K.; Zhang, S. Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses. Proc. Natl. Acad. Sci. USA, 2008, 105, 15726-15731.
29. Corin, K.; Pick, H.; Baaske, P.; Cook, B.L.; Duhr, S.; Wienken, C.J.; Braun, D.; Vogel, H.; Zhang, S. Insertion of T4-lysozyme (T4L) can be a useful tool for studying olfactory-related GPCRs. Mol. Biosyst., 2012, 8, 1750-1759.
30. Reeves, P.J.; Kim, J.M.; Khorana, H.G. Structure and function in rhodopsin: a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants. Proc. Natl. Acad. Sci. USA, 2002, 99, 13413-13418.
31. Ishihara, G.; Goto, M.; Saeki, M.; Ito, K.; Hori, T.; Kigawa, T.; Shirouzu, M.; Yokoyama, S. Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin- fusion vectors. Protein Expres. Purif., 2005, 41, 27-37.
32. Cook, B.L.; Ernberg, K.E.; Chung, H.; Zhang, S. Study of a synthetic human olfactory receptor 17-4: expression and purification from an inducible mammalian cell line. PLoS ONE, 2008, 3, e2920.
33. Cook, B.L.; Steuerwald, D.; Kaiser, L.; Graveland-Bikker, J.; Vanberghem, M.; Berke, A.P.; Herlihy, K.; Pick, H.; Vogel, H.; Zhang, S. Large-scale production and study of a synthetic G proteincoupled receptor: human olfactory receptor 17-4. Proc. Natl. Acad. Sci. USA, 2009, 106, 11925-11930.
34. Ren, H.; Yu, D.; Ge, B.; CooK, B.L.; Xu, Z.; Zhang, S. High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1. PLoS ONE, 2009, 4, e4509.
35. Nekrasova, E.; Sosinskaya, A.; Natochin, M.; Lancet, D.; Gat, U. Overexpression, solubilization and purification of rat and human olfactory receptors. Eur. J. Biochem., 1996, 238, 28-37.
36. Katada, S.; Nakagawa, T.; Kataoka, H.; Touhara, K. Odorant response assays for a heterologously expressed olfactory receptor. Biochem. Biophys. Res. Commun., 2003, 305, 964-969.
37. Wang, X.; Zhang, S. Production of a bioengineered G-protein coupled receptor of human formyl peptide receptor 3. PLoS ONE, 2011, 6, e23076.
38. Grisshammer, R.; White, J.F.; Trinh, L.B.; Shiloach, J. Large-scale expression and purification of a G-protein-coupled receptor for structure determination-An overview, J. Struct. Funct. Genomics, 2005, 6, 159-163.
39. Matthews, B.W.; Remington, S.J. The three dimensional structure of the lysozyme from bacteriophage T4. Proc. Natl. Acad. Sci. USA, 1974, 71, 4178-4182.