Expression of an olfactory receptor in Escherichia coli: Purification, reconstitution, and ligand binding

Biochemistry

Volumn 35, Issue 50, 1996, Pages 16077-16084

  Kiefer, Hans ^a,d   Krieger, Jürgen ^b   Olszewski, John D ^c,e   Von Heijne, Gunnar ^a   Prestwich, Glenn D ^c,f   Breer, Heinz ^b  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b UNIVERSITY OF STUTTGART   (Germany)

^c STONY BROOK UNIVERSITY   (United States)

^d UNIVERSITY OF HOHENHEIM   (Germany)

^e PFIZER INC   (United States)

^f UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; RECEPTOR PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; CELL INCLUSION; CIRCULAR DICHROISM; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL ELECTROPHORESIS; IMMUNOBLOTTING; LIGAND BINDING; NONHUMAN; OLFACTORY RECEPTOR; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SOLUBILIZATION; SPECTROFLUOROMETRY;

AMINO ACID SEQUENCE; ANIMALS; CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; MODELS, STRUCTURAL; ODORS; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, CELL SURFACE; RECEPTORS, ODORANT; RECOMBINANT FUSION PROTEINS; SEQUENCE TAGGED SITES;

EID: 0030467888     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9612069     Document Type: Article

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