Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents

Protein Science

Volumn 20, Issue 6, 2011, Pages 1030-1041

  Klammt, Christian ^a,b   Perrin, Marilyn H ^c   Maslennikov, Innokentiy ^a,b   Renault, Ludovic ^d   Krupa, Martin ^a   Kwiatkowski, Witek ^a   Stahlberg, Henning ^d   Vale, Wylie ^c   Choe, Senyon ^a,b  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b Gachon University College of Medicine   (South Korea)

^c SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cell free; CRFR; Family B GPCR; Functional ligand binding; Integral membrane protein; NMR; Polymer; Static light scattering

Indexed keywords

CORTICOTROPIN RELEASING FACTOR; DETERGENT; FRUCTOSE; G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; POLYMER;

ANIMAL EXPERIMENT; ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; HUMAN; HUMAN CELL; IN VITRO STUDY; LIGAND BINDING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TARGETING;

CELL-FREE SYSTEM; DETERGENTS; FRUCTANS; GENE EXPRESSION; HUMANS; LIGANDS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; POLYMERS; PROTEIN BINDING; RECEPTORS, G-PROTEIN-COUPLED;

EID: 79956197327     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.636     Document Type: Article

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