Highly Selective Separation of Rhodopsin from Bovine Rod Outer Segment Membranes Using Combination of Divalent Cation and Alkyl(thio)glucoside

Photochemistry and Photobiology

Volumn 67, Issue 5, 1998, Pages 495-499

  Okada, Tetsuji ^a   Takeda, Kazuki ^a   Kouyama, Tsutomu ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINAE;

DIVALENT CATION; RHODOPSIN; THIOGLYCOSIDE;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; ISOLATION AND PURIFICATION; RETINA ROD OUTER SEGMENT;

ANIMALS; CATIONS, DIVALENT; CATTLE; RHODOPSIN; ROD OUTER SEGMENTS; THIOGLUCOSIDES;

EID: 0032059717     PISSN: 00318655     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1751-1097.1998.tb09445.x     Document Type: Article

References (21)

1. Baylar, D. (1996) How photons start vision. Proc. Natl. Acad. Sci. USA 93, 560-565.
2. De Grip, W. J. (1982) Thermal stability of rhodopsin and opsin in some novel detergents. Methods Enzymol. 81, 256-265.
3. Helenius, A. and K. Simons (1975) Solubilization of membranes by detergents. Biochim. Biophys. Acta 415, 29-79.
4. Vinson, P. K., Y. Talmon and A. Walter (1989) Vesicle-micelle transition of phosphatidylcholine and octyl glucoside elucidated by cryo-transmission electron microscopy. Biophys. J. 56, 669-681.
5. 8. J. Colloid Interface Sci. 147, 1-21.
6. Walter, A., P. K. Vinson, A. Kaplun and Y. Talmon (1991) Intermediate structures in the cholate-phosphatidylcholine vesicle-micelle transition. Biophys. J. 60, 1315-1325.
7. Lasch, J. (1995) Interaction of detergents with lipid vesicles. Biochim. Biophys. Acta 1241, 726-738.
8. Kühlbrandt, W. (1987) Three-dimensional crystals of the light-harvesting chlorophyll a/b protein complex from pea chloroplasts J. Mol. Biol. 194, 757-762.
9. Kouyama, T., M. Yamamoto, N. Kamiya, H. Iwasaki, T. Ueki and I. Sakurai (1994) Polyhedral assembly of a membrane protein in its three-dimensional crystal. J. Mol. Biol. 236, 990-994.
10. Nakazato, K., C. Toyoshima, I. Enami and Y. Inoue (1996) Two-dimensional crystallization and cryo-electron microscopy of photosystem II. J. Mol. Biol. 257, 225-232.
11. Karrasch, S., D. Typke, T. Walz, M. Miller, G. Tsiotis and A. Engel (1996) Highly ordered two-dimensional crystals of photosystem I reaction center from Synechococcus sp.: functional and structural analyses. J. Mol. Biol. 262, 336-348.
12. Okada, T., T. Matsuda, H. Kandori, Y. Fukada, T. Yoshizawa and Y. Shichida (1994) Circular dichroism of metaiodopsin II and its binding to transducin: a comparative study between meta II intermediates of iodopsin and rhodopsin. Biochemistry 33, 4940-4946.
13. Bartlett, G. R. (1959) Phosphorus assay in column chromatography. J. Biol. Chem. 234, 466-468.
14. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
15. Miljanich, G. P., P. P. Nemes, D. L. White and E. A. Dratz (1981) The asymmetric transmembrane distribution of phosphatidylethanolamine, phosphatidylserine, and fatty acids of the bovine retinal rod outer segment disk membrane. J. Membr. Biol. 60, 249-255.
16. Stubbs, G. W. and B. J. Litman (1978) Effect of alterations in the amphipathic microenvironment on the conformational stability of bovine opsin. 1. Mechanism of solubilization of disk membranes by the nonionic detergent, octyl glucoside. Biochemistry 17, 215-219.
17. DeGrip, W. J. (1982) Purification of bovine rhodopsin over concanavalin A-Sepharose. Methods Enzymol. 81, 197-207.
18. Lin, S. W. and T. P. Sakmar (1996) Specific tryptophan UV-absorbance changes are probes of the transition of rhodopsin to its active state. Biochemistry 35, 11149-11159.
19. Farrens, D. L., C. Altenbach, K. Yang, W. L. Hubbell and H. G. Khorana (1996) Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin. Science 274, 768-770.
20. Schott, H. (1995) Hydrophilic-lipophilic balance, solubility parameter, and oil-water partition coefficient as universal parameters of nonionic surfactants. J. Pharm. Sci. 84, 1215-1222.
21. Tsui, F. C., S. A. Sundberg and W. L. Hubbell (1990) Distribution of charge on photoreceptor disc membranes and implications for charged lipid asymmetry. Biophys. J. 57, 85-97.