Isotopic labeling of mammalian G protein-coupled receptors heterologously expressed in Caenorhabditis elegans

Analytical Biochemistry

Volumn 472, Issue , 2015, Pages 30-36

  Salom, David ^a,b   Cao, Pengxiu ^b   Yuan, Yiyuan ^b   Miyagi, Masaru ^b   Feng, Zhaoyang ^b   Palczewski, Krzysztof ^b  

^a Polgenix Inc   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Caenorhabditis elegans; G protein coupled receptors (GPCRs); Heterologous expression; Isotopic labeling; Nuclear magnetic resonance (NMR); Rhodopsin

Indexed keywords

BACTERIA; HEAVY WATER; ISOTOPES; MAMMALS; NUCLEAR MAGNETIC RESONANCE;

CAENORHABDITIS ELEGANS; G PROTEIN COUPLED RECEPTORS; HETEROLOGOUS EXPRESSION; ISOTOPIC LABELING; NUCLEAR MAGNETIC RESONANCE(NMR); RHODOPSIN;

PROTEINS;

CARBON 13; DEUTERIUM OXIDE; G PROTEIN COUPLED RECEPTOR; NITROGEN 15; RHODOPSIN; RECOMBINANT PROTEIN;

ARTICLE; CAENORHABDITIS ELEGANS; FEASIBILITY STUDY; GENE EXPRESSION SYSTEM; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; ISOMERIZATION; ISOTOPE LABELING; MICROBIAL GROWTH; NONHUMAN; PARASITE VIABILITY; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN STRUCTURE; ANIMAL; BIOSYNTHESIS; BOVINE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; NUCLEAR MAGNETIC RESONANCE; TRANSGENIC ANIMAL;

BACTERIA (MICROORGANISMS); BOVINAE; CAENORHABDITIS ELEGANS; EUKARYOTA; MAMMALIA;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; CATTLE; ESCHERICHIA COLI; GENE EXPRESSION; ISOTOPE LABELING; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; RECOMBINANT PROTEINS; RHODOPSIN;

EID: 84921047427     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.11.008     Document Type: Article

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