Purification of the human G protein-coupled receptor adenosine A2aR in a stable and functional form expressed in Pichia pastoris

Current Protocols in Protein Science

Volumn 1, Issue SUPPL.67, 2012, Pages

  Singh, Shweta ^a   Zhang, Minghao ^a   Bertheleme, Nicolas ^a   Strange, Philip G ^b   Byrne, Bernadette ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF READING   (United Kingdom)

Author keywords

Adenosine A2aR; Aggregation; Antibody affinity chromatography; Coupled receptor; G protein; Immobilized metal affinity chromatography; Ion exchange; Radioligand binding analysis; SDS PAGE; Size exclusion chromatography

Indexed keywords

ADENOSINE A2A RECEPTOR; BUFFER; DETERGENT; G PROTEIN COUPLED RECEPTOR; PROTEINASE; RESIN; VIRUS ENZYME; HYBRID PROTEIN; MEMBRANE PROTEIN;

ANTIBODY AFFINITY; ARTICLE; CLINICAL PROTOCOL; CONCENTRATION (PARAMETERS); EXPERIMENTAL DESIGN; GEL PERMEATION CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROCESS DESIGN; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STABILITY; RECEPTOR BINDING; RECEPTOR SENSITIVITY; TOBACCO VIRUS; VIRUS; AFFINITY CHROMATOGRAPHY; CHEMISTRY; GEL CHROMATOGRAPHY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; PICHIA; PROTEIN ENGINEERING; SOLUBILITY;

PICHIA PASTORIS;

CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MEMBRANE PROTEINS; PICHIA; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEOLYSIS; RECEPTOR, ADENOSINE A2A; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

EID: 84861132421     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps2904s67     Document Type: Article

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