Production of membrane proteins without cells or detergents

New Biotechnology

Volumn 28, Issue 3, 2011, Pages 250-254

  Rajesh, Sundaresan ^a   Knowles, Timothy ^a   Overduin, Michael ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL-BASED; CELL-FREE EXPRESSION; CELLULAR SYSTEM; FUNCTIONAL MEMBRANES; HYDROPHOBIC ENVIRONMENT; HYDROPHOBIC NATURE; LIPID PARTICLES; MALEIC ACIDS; MEMBRANE PROTEINS; MIMETICS; MISFOLDING; NANODISCS; OPTIMISATIONS; PROTEIN AGGREGATION; SOLUBILISATION;

BIOLOGICAL MEMBRANES; HYDROPHOBICITY; SOAPS (DETERGENTS); STYRENE;

PROTEINS;

LIPID; MALEIC ACID DERIVATIVE; MEMBRANE PROTEIN; NANODISC; STYRENE DERIVATIVE;

ARTICLE; BIOMIMETICS; CELL FREE SYSTEM; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; WHEAT;

CELL-FREE SYSTEM; DETERGENTS; ESCHERICHIA COLI; MEMBRANE PROTEINS; PROTEIN FOLDING; TRITICUM;

EID: 79953702800     PISSN: 18716784     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nbt.2010.07.011     Document Type: Article

References (45)

1. Lundstrom K. Structural genomics and drug discovery. J. Cell. Mol. Med. 2007, 11:224-238.
2. Junge F., Schneider B., Reckel S., Schwarz D., et al. Large-scale production of functional membrane proteins. Cell. Mol. Life Sci. 2008, 65:1729-1755.
3. Sobhanifar S., Reckel S., Junge F., Schwarz D., et al. Cell-free expression and stable isotope labelling strategies for membrane proteins. J. Biomol. NMR 2010, 46:33-43.
4. Endo Y., Takai K., Ueda T. Cell-free protein production: methods and protocols. Methods Mol. Biol. 2010, Vol. 607. Humana Press.
5. Schwarz D., Junge F., Durst F., Frolich N., et al. Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. Nat. Protoc. 2007, 2:2945-2957.
6. Endo Y., Sawasaki T. High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system. Biotechnol Adv. 2003, 21:695-713.
7. Klammt C., Schwarz D., Fendler K., Haase W., et al. Evaluation of detergents for the soluble expression of a-helical and b-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J. 2005, 272:6024-6038.
8. Elbaz Y., Steiner-Mordoch S., Danieli T., Schuldiner S. In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state. Proc. Natl. Acad. Sci. U. S. A. 2004, 101:1519-1524.
9. Opekarová M., Tanner W. Specific lipid requirements of membrane proteins - a putative bottleneck in heterologous expression. Biochim. Biophys. Acta: Biomembr. 2003, 1610:11-22.
10. Berrier C., Park K.-H., Abes S., Bibonne A., et al. Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. Biochemistry 2004, 43:12585-12591.
11. Shimada Y., Wang Z.-Y., Mochizuki Y., Kobayashi M., et al. Functional expression and characterization of a bacterial light-harvesting membrane protein in Escherichia coli and cell-free synthesis systems. Biosci. Biotechnol. Biochem. 2004, 68:1942-1948.
12. Ishihara G., Goto M., Saeki M., Ito K., et al. Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors. Protein Expr. Purif. 2005, 41:27-37.
13. Park K.-H., Berrier C., Lebaupain F., Pucci B., et al. Fluorinated and hemifluorinated surfactants as alternatives to detergents for membrane protein cell-free synthesis. Biochem. J. 2007, 403:183-187.
14. Kaiser L., Graveland-Bikker J., Steurwald D., Vanberghem M.I., et al. Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses. Proc. Natl. Acad. Sci. U. S. A. 2008, 105:15726-15731.
15. Phillips R., Ursell T., Wiggins P., Sens P. Emerging roles for lipids in shaping membrane-protein function. Nature 2009, 459:379-385.
16. Keller T., Schwarz D., Bernhard F., Dötsch V., et al. Cell free expression and functional reconstitution of eukaryotic drug transporters. Biochemistry 2008, 47:4552-4564.
17. Nozawa A., Nanamiya H., Miyata T., Linka N., et al. A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters. Plant Cell. Physiol. 2007, 48:1815-1820.
18. Goren M.A., Nozawa A., Makino S.I., Wrobel R.L., et al. Cell-free translation of integral membrane proteins into unilamellar liposomes. Methods Enzymol. 2009, 463:647-673.
19. Lehner I., Basting D., Meyer B., Haase W., et al. The key residue for substrate transport (Glu14) in the EmrE dimer is asymmetric. J. Biol. Chem. 2008, 283:3281-3288.
20. Kalmbach R., Chizhov I., Schumacher M.C., Friedrich T., et al. Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomes. J. Mol. Biol. 2007, 371:639-648.
21. Goren M.A., Fox B.G. Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. Protein Expr. Purif. 2008, 62:171-178.
22. Rosenberg R.L., East J. Cell-free expression of functional Shaker potassium channels. Nature 1992, 360:166-1699.
23. Kuruma Y., Nishiyama K., Shimizu Y., Müller M., et al. Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins. Biotechnol. Prog. 2005, 21:1243-1251.
24. Wuu J.J., Swartz J.R. High yield cell-free production of integral membrane proteins without refolding or detergents. Biochim. Biophys. Acta: Biomembr. 2008, 1778:1237-1250.
25. Bayburt T.H., Sligar S.G. Membrane protein assembly into nanodiscs. FEBS Lett. 2010, 584:1721-1727.
26. Popot J.L. Amphipols, nanodiscs, and fluorinated surfactants: three nonconventional approaches to studying membrane proteins in aqueous solutions. Annu. Rev. Biochem 2010, 79. 13.1-13.9.
27. Nath A., Atkins W.M., Sligar S.G. Applications of phospholipid bilayer nanodiscs in the study of membranes and membrane proteins. Biochemistry 2007, 46:2059-2069.
28. Katzen F., Fletcher J.E., Yang J.-P., Kang D., et al. Insertion of membrane proteins into discoidal membranes using a cell-free protein expression approach. J. Proteome Res. 2008, 7:3535-3542.
29. Alami M., Dalal K., Lelj-Garolla B., Sligar S.G., et al. Nanodiscs unravel the interaction between the SecYEG channel and its cytosolic partner SecA. EMBO J. 2007, 26:1995-2004.
30. Cappuccio J.A., Blanchette C.D., Sulchek T.A., Arroyo E.S., et al. Cell-free co-expression of functional membrane proteins and apolipoprotein forming soluble nanolipoprotein particles. Mol. Cell. Proteomics 2008, 7:2246-2253.
31. Bowie J.U. Stabilizing membrane proteins. Curr. Opin. Struct. Biol. 2001, 11(4):397-402.
32. McGregor C.L., Chen L., Pomroy N.C., Hwang P., et al. Lipopeptide detergents designed for the structural study of membrane proteins. Nat. Biotechnol. 2003, 21(2):171-176.
33. Popot J.L., Berry E.A., Charvolin D., Creuzenet C., et al. Amphipols: polymeric surfactants for membrane biology research. Cell. Mol. Life Sci. 2003, 60:1559-1574.
34. Sanders C.R., Kuhn Hoffmann A., Gray D.N., Keyes M.H., et al. French swimwear for membrane proteins. Chembiochem 2004, 5:423-426.
35. Tribet C., Audebert R., Popot J.L. Amphipols: polymers that keep membrane proteins soluble in aqueous solutions. Proc. Natl. Acad. Sci. U. S. A. 1996, 93:15047-15050.
36. Diab C., Tribet C., Gohon Y., Popot J.L., et al. Complexation of integral membrane proteins by phosphorylcholine-based amphipols. Biochim. Biophys. Acta. 2007, 1768:2737-2747.
37. Gorzelle B.M., Hoffman A.K., Keyes M.H., Gray D.N., et al. Amphipols can support the activity of a membrane enzyme. J. Am. Chem. Soc. 2002, 124:11594-11595.
38. Knowles T.J., Finka R., Smith C., Lin Y.-P., et al. Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. J. Am. Chem. Soc. 2009, 131:7484-7485.
39. 2+-ATPase. J. Biol. Chem. 2000, 275:18623-18637.
40. Dahmane T., Damian M., Mary S., Popot J.L., et al. Amphipol-assisted in vitro folding of G protein-coupled receptors. Biochemistry 2009, 48:6516-6521.
41. 2+-ATPase from sarcoplasmic reticulum: a comparative study. Biochemistry 2006, 45:1861-1869.
42. Catoire L.J., Zoonens M., van Heijenoort C., Giusti F., et al. Solution NMR mapping of water-accessible residues in the transmembrane beta-barrel of OmpX. Eur. Biophys. J. 2010, 39:623-630.
43. Nagy J.K., Kuhn-Hoffmann A., Keyes M.H., Gray D.N., et al. Use of amphipathic polymers to deliver a membrane protein to lipid bilayers. FEBS Lett. 2001, 501:115-120.
44. Chen Y.J., Pornillos O., Lieu S., Ma C., et al. X-ray structure of EmrE supports dual topology model. Proc. Natl. Acad. Sci. U. S. A. 2007, 104:18999-19004.
45. Maslennikov I., Klammt C., Hwang E., Kefala G., et al. Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis. Proc. Natl. Acad. Sci. U. S. A. 2010, 107:10902-10907.