Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Shiroishi, Mitsunori ^a,b,c   Tsujimoto, Hirokazu ^a,c   Makyio, Hisayoshi ^a   Asada, Hidetsugu ^a,c   Yurugi Kobayashi, Takami ^a,c   Shimamura, Tatsuro ^a,c   Murata, Takeshi ^a   Nomura, Norimichi ^a   Haga, Tatsuya ^d   Iwata, So ^a,c,e   Kobayashi, Takuya ^a,c  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c KYOTO UNIVERSITY FACULTY OF MEDICINE   (Japan)

^d GAKUSHUIN UNIVERSITY   (Japan)

^e IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

G protein coupled receptor; High expression; Membrane protein; Receptor variants; Saccharomyces cerevisiae; Screening; Structural study

Indexed keywords

ADENOSINE A2A RECEPTOR; ADENOSINE RECEPTOR; ADRENERGIC RECEPTOR; BETA 2 ADRENERGIC RECEPTOR; CHOLINERGIC RECEPTOR; DOPAMINE 1 RECEPTOR; DOPAMINE 2 RECEPTOR; DOPAMINE 4 RECEPTOR; DOPAMINE RECEPTOR; G PROTEIN COUPLED RECEPTOR; HISTAMINE H1 RECEPTOR; HISTAMINE H3 RECEPTOR; HISTAMINE H4 RECEPTOR; NEUROPEPTIDE 1 RECEPTOR; NEUROPEPTIDE 2 RECEPTOR; NEUROPEPTIDE 4 RECEPTOR; NEUROPEPTIDE 5 RECEPTOR; NEUROPEPTIDE RECEPTOR; NEUROTENSIN RECEPTOR; NEUROTENSIN RECEPTOR 1; NEUROTENSIN RECEPTOR 2; OPIATE RECEPTOR; PROSTANOID RECEPTOR; PROTEIN HCRM2; PROTEIN VARIANT; SEROTONIN 1B RECEPTOR; SEROTONIN 1D RECEPTOR; TACHYKININ RECEPTOR 1; TACHYKININ RECEPTOR 2; UNCLASSIFIED DRUG; UNINDEXED DRUG;

ARTICLE; CRYSTALLOGRAPHY; EVALUATION; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; SCREENING;

CRYSTALLIZATION; GREEN FLUORESCENT PROTEINS; HUMANS; PICHIA; PLASMIDS; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, HISTAMINE H1; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 84862239975     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-78     Document Type: Article

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