-
1
-
-
80053163752
-
-
Springer Netherlands Dordrecht
-
RC, W., JN, T.J., Mutation and evolution, vol. 7, 1998, Springer Netherlands, Dordrecht, 10.1007/978-94-011-5210-5.
-
(1998)
Mutation and evolution
, vol.7
-
-
RC, W.1
JN, T.J.2
-
2
-
-
84963642794
-
Directed evolution and synthetic biology applications to microbial systems
-
Bassalo, M.C., Liu, R., Gill, R.T., Directed evolution and synthetic biology applications to microbial systems. Curr Opin Biotechnol 39 (2016), 126–133, 10.1016/j.copbio.2016.03.016.
-
(2016)
Curr Opin Biotechnol
, vol.39
, pp. 126-133
-
-
Bassalo, M.C.1
Liu, R.2
Gill, R.T.3
-
3
-
-
77955090647
-
Evolution of the mutation rate
-
Lynch, M., Evolution of the mutation rate. Trends Genet 26 (2010), 345–352, 10.1016/j.tig.2010.05.003.
-
(2010)
Trends Genet
, vol.26
, pp. 345-352
-
-
Lynch, M.1
-
4
-
-
84982085494
-
Tempo and mode of genome evolution in a 50,000-generation experiment
-
Tenaillon, O., Barrick, J.E., Ribeck, N., Deatherage, D.E., Blanchard, J.L., Dasgupta, A., et al. Tempo and mode of genome evolution in a 50,000-generation experiment. Nature 536 (2016), 165–170, 10.1038/nature18959.
-
(2016)
Nature
, vol.536
, pp. 165-170
-
-
Tenaillon, O.1
Barrick, J.E.2
Ribeck, N.3
Deatherage, D.E.4
Blanchard, J.L.5
Dasgupta, A.6
-
5
-
-
33747830026
-
Artificial transmutation of the gene
-
Muller, H.J., Artificial transmutation of the gene. Science (80-) 66 (1927), 84–87, 10.1126/science.66.1699.84.
-
(1927)
Science (80-)
, vol.66
, pp. 84-87
-
-
Muller, H.J.1
-
6
-
-
33748605946
-
Genetic effects of ultra-violet radiation in maize: I. Unfiltered radiation
-
Stadler, L.J., Sprague, G.F., Genetic effects of ultra-violet radiation in maize: I. Unfiltered radiation. Proc Natl Acad Sci 22 (1936), 572–578, 10.1073/pnas.22.10.572.
-
(1936)
Proc Natl Acad Sci
, vol.22
, pp. 572-578
-
-
Stadler, L.J.1
Sprague, G.F.2
-
7
-
-
84903816810
-
Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool
-
Zhang, X., Zhang, X.F., Li, H.P., Wang, L.Y., Zhang, C., Xing, X.H., et al. Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool. Appl Microbiol Biotechnol 98 (2014), 5387–5396, 10.1007/s00253-014-5755-y.
-
(2014)
Appl Microbiol Biotechnol
, vol.98
, pp. 5387-5396
-
-
Zhang, X.1
Zhang, X.F.2
Li, H.P.3
Wang, L.Y.4
Zhang, C.5
Xing, X.H.6
-
8
-
-
0000511472
-
Chemical production of mutations
-
Auerbach, C., Robson, J.M., Chemical production of mutations. Nature 105 (1947), 243–247, 10.1126/science.105.2723.243.
-
(1947)
Nature
, vol.105
, pp. 243-247
-
-
Auerbach, C.1
Robson, J.M.2
-
9
-
-
33745232539
-
EMS mutagenesis of arabidopsis
-
Humana Press New Jersey
-
Kim, Y., Schumaker, K.S., Zhu, J.-K., EMS mutagenesis of arabidopsis. Arab. Protoc., vol. 323, 2006, Humana Press, New Jersey, 101–103, 10.1385/1-59745-003-0:101.
-
(2006)
Arab. Protoc.
, vol.323
, pp. 101-103
-
-
Kim, Y.1
Schumaker, K.S.2
Zhu, J.-K.3
-
10
-
-
0029680681
-
An efficient random mutagenesis technique using an E. coli mutator strain
-
Humana Press New Jersey
-
Greener, A., Callahan, M., Jerpseth, B., An efficient random mutagenesis technique using an E. coli mutator strain. Vitr. Mutagen. Protoc., vol. 57, 1996, Humana Press, New Jersey, 375–385, 10.1385/0-89603-332-5:375.
-
(1996)
Vitr. Mutagen. Protoc.
, vol.57
, pp. 375-385
-
-
Greener, A.1
Callahan, M.2
Jerpseth, B.3
-
11
-
-
79955534060
-
A system for the continuous directed evolution of biomolecules
-
Esvelt, K.M., Carlson, J.C., Liu, D.R., A system for the continuous directed evolution of biomolecules. Nature 472 (2011), 499–503, 10.1038/nature09929.
-
(2011)
Nature
, vol.472
, pp. 499-503
-
-
Esvelt, K.M.1
Carlson, J.C.2
Liu, D.R.3
-
12
-
-
84943777049
-
Development of potent in vivo mutagenesis plasmids with broad mutational spectra
-
Badran, A.H., Liu, D.R., Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Nat Commun, 6, 2015, 8425, 10.1038/ncomms9425.
-
(2015)
Nat Commun
, vol.6
, pp. 8425
-
-
Badran, A.H.1
Liu, D.R.2
-
13
-
-
1542513556
-
Mobile elements: drivers of genome evolution
-
Kazazian, H.H., Mobile elements: drivers of genome evolution. Science 303 (2004), 1626–1632, 10.1126/science.1089670.
-
(2004)
Science
, vol.303
, pp. 1626-1632
-
-
Kazazian, H.H.1
-
14
-
-
84857635290
-
Random UV-C mutagenesis of ScheVersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars
-
Hughes, S.R., Gibbons, W.R., Bang, S.S., Pinkelman, R., BischoV, K.M., Slininger, P.J., et al. Random UV-C mutagenesis of ScheVersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars. J Ind Microbiol Biotechnol 39 (2012), 163–173, 10.1007/s10295-011-1012-x.
-
(2012)
J Ind Microbiol Biotechnol
, vol.39
, pp. 163-173
-
-
Hughes, S.R.1
Gibbons, W.R.2
Bang, S.S.3
Pinkelman, R.4
BischoV, K.M.5
Slininger, P.J.6
-
15
-
-
84884601555
-
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
-
Luan, G., Cai, Z., Li, Y., Ma, Y., Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production. Biotechnol Biofuels, 6, 2013, 137, 10.1186/1754-6834-6-137.
-
(2013)
Biotechnol Biofuels
, vol.6
, pp. 137
-
-
Luan, G.1
Cai, Z.2
Li, Y.3
Ma, Y.4
-
16
-
-
84940954593
-
Comparative genome analysis of a thermotolerant Escherichia coli obtained by Genome Replication Engineering Assisted Continuous Evolution (GREACE) and its parent strain provides new understanding of microbial heat tolerance
-
Luan, G., Bao, G., Lin, Z., Li, Y., Chen, Z., Li, Y., et al. Comparative genome analysis of a thermotolerant Escherichia coli obtained by Genome Replication Engineering Assisted Continuous Evolution (GREACE) and its parent strain provides new understanding of microbial heat tolerance. N Biotechnol 32 (2015), 732–738, 10.1016/j.nbt.2015.01.013.
-
(2015)
N Biotechnol
, vol.32
, pp. 732-738
-
-
Luan, G.1
Bao, G.2
Lin, Z.3
Li, Y.4
Chen, Z.5
Li, Y.6
-
17
-
-
84940521020
-
Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast
-
Huang, M., Bai, Y., Sjostrom, S.L., Hallström, B.M., Liu, Z., Petranovic, D., et al. Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast. Proc Natl Acad Sci U. S. A 112 (2015), E4689–E4696, 10.1073/pnas.1506460112.
-
(2015)
Proc Natl Acad Sci U. S. A
, vol.112
, pp. E4689-E4696
-
-
Huang, M.1
Bai, Y.2
Sjostrom, S.L.3
Hallström, B.M.4
Liu, Z.5
Petranovic, D.6
-
18
-
-
84874482336
-
Coupling mutagenesis and parallel deep sequencing to probe essential residues in a genome or gene
-
Robins, W.P., Faruque, S.M., Mekalanos, J.J., Coupling mutagenesis and parallel deep sequencing to probe essential residues in a genome or gene. Pnas 110 (2013), E848–E857, 10.1073/pnas.1222538110.
-
(2013)
Pnas
, vol.110
, pp. E848-E857
-
-
Robins, W.P.1
Faruque, S.M.2
Mekalanos, J.J.3
-
19
-
-
84929510811
-
Integrating chemical mutagenesis and whole-genome sequencing as a platform for forward and reverse genetic analysis of Chlamydia
-
Kokes, M., Dunn, J.D., Granek, J.A., Nguyen, B.D., Barker, J.R., Valdivia, R.H., et al. Integrating chemical mutagenesis and whole-genome sequencing as a platform for forward and reverse genetic analysis of Chlamydia. Cell Host Microbe 17 (2015), 716–725, 10.1016/j.chom.2015.03.014.
-
(2015)
Cell Host Microbe
, vol.17
, pp. 716-725
-
-
Kokes, M.1
Dunn, J.D.2
Granek, J.A.3
Nguyen, B.D.4
Barker, J.R.5
Valdivia, R.H.6
-
20
-
-
0345369642
-
Rapid identification of essential and nonessential herpesvirus genes by direct transposon mutagenesis
-
Brune, W., Ménard, C., Hobom, U., Odenbreit, S., Messerle, M., Koszinowski, U.H., Rapid identification of essential and nonessential herpesvirus genes by direct transposon mutagenesis. Nat Biotechnol 17 (1999), 360–364, 10.1038/7914.
-
(1999)
Nat Biotechnol
, vol.17
, pp. 360-364
-
-
Brune, W.1
Ménard, C.2
Hobom, U.3
Odenbreit, S.4
Messerle, M.5
Koszinowski, U.H.6
-
21
-
-
0000812428
-
Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature-tagged transposon mutagenesis
-
Camacho, L.R., Ensergueix, D., Perez, E., Gicquel, B., Guilhot, C., Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature-tagged transposon mutagenesis. Mol Microbiol 34 (1999), 257–267, 10.1046/j.1365-2958.1999.01593.x.
-
(1999)
Mol Microbiol
, vol.34
, pp. 257-267
-
-
Camacho, L.R.1
Ensergueix, D.2
Perez, E.3
Gicquel, B.4
Guilhot, C.5
-
22
-
-
73649150958
-
The maintenance of the accuracy of protein synthesis and its relevance to ageing
-
ORGEL, L.E., The maintenance of the accuracy of protein synthesis and its relevance to ageing. Proc Natl Acad Sci U. S. A 49 (1963), 517–521, 10.1073/PNAS.49.4.517.
-
(1963)
Proc Natl Acad Sci U. S. A
, vol.49
, pp. 517-521
-
-
ORGEL, L.E.1
-
23
-
-
0346265834
-
Mechanisms of mutagenesis in the Escherichia coli mutator mutD5: role of DNA mismatch repair
-
Schaaper, R.M., Mechanisms of mutagenesis in the Escherichia coli mutator mutD5: role of DNA mismatch repair. Proc Natl Acad Sci U. S. A 85 (1988), 8126–8130, 10.1073/pnas.85.21.8126.
-
(1988)
Proc Natl Acad Sci U. S. A
, vol.85
, pp. 8126-8130
-
-
Schaaper, R.M.1
-
24
-
-
9244225098
-
Mutants in the Exo I motif of Escherichia coli dnaQ: defective proofreading and inviability due to error catastrophe
-
Fijalkowska, I.J., Schaaper, R.M., Mutants in the Exo I motif of Escherichia coli dnaQ: defective proofreading and inviability due to error catastrophe. Proc Natl Acad Sci U. S. A 93 (1996), 2856–2861.
-
(1996)
Proc Natl Acad Sci U. S. A
, vol.93
, pp. 2856-2861
-
-
Fijalkowska, I.J.1
Schaaper, R.M.2
-
25
-
-
84919359797
-
Evolution-guided optimization of biosynthetic pathways
-
Raman, S., Rogers, J.K., Taylor, N.D., Church, G.M., Evolution-guided optimization of biosynthetic pathways. Proc Natl Acad Sci, 111, 2014, 201409523, 10.1073/pnas.1409523111.
-
(2014)
Proc Natl Acad Sci
, vol.111
, pp. 201409523
-
-
Raman, S.1
Rogers, J.K.2
Taylor, N.D.3
Church, G.M.4
-
26
-
-
77956328000
-
Bacterial charity work leads to population-wide resistance
-
Lee, H.H., Molla, M.N., Cantor, C.R., Collins, J.J., Bacterial charity work leads to population-wide resistance. Nature 467 (2010), 82–85, 10.1038/nature09354.
-
(2010)
Nature
, vol.467
, pp. 82-85
-
-
Lee, H.H.1
Molla, M.N.2
Cantor, C.R.3
Collins, J.J.4
-
27
-
-
84555171441
-
Evolutionary paths to antibiotic resistance under dynamically sustained drug selection
-
Toprak, E., Veres, A., Michel, J.-B., Chait, R., Hartl, D.L., Kishony, R., Evolutionary paths to antibiotic resistance under dynamically sustained drug selection. Nat Genet 44 (2012), 101–105, 10.1038/ng.1034.
-
(2012)
Nat Genet
, vol.44
, pp. 101-105
-
-
Toprak, E.1
Veres, A.2
Michel, J.-B.3
Chait, R.4
Hartl, D.L.5
Kishony, R.6
-
28
-
-
0002694056
-
A Method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
-
Leung, D.W., Chen, E., Goeddel, D.V., A Method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique 1 (1989), 11–15.
-
(1989)
Technique
, vol.1
, pp. 11-15
-
-
Leung, D.W.1
Chen, E.2
Goeddel, D.V.3
-
29
-
-
85045502002
-
Randomization of genes by PCR mutagenesis
-
Cadwell, R.C., Joyce, G.F., Randomization of genes by PCR mutagenesis. Genome Res 2 (1992), 28–33, 10.1101/gr.2.1.28.
-
(1992)
Genome Res
, vol.2
, pp. 28-33
-
-
Cadwell, R.C.1
Joyce, G.F.2
-
30
-
-
0026649231
-
Directed evolution of an RNA enzyme
-
Beaudry, A.A., Joyce, G.F., Directed evolution of an RNA enzyme. Science 257 (1992), 635–641, 10.1126/science.1496376.
-
(1992)
Science
, vol.257
, pp. 635-641
-
-
Beaudry, A.A.1
Joyce, G.F.2
-
31
-
-
0027488624
-
Isolation of new ribozymes from a large pool of random sequences
-
Bartel, D.P., Szostak, J.W., Isolation of new ribozymes from a large pool of random sequences. Science (80-) 261 (1993), 1411–1418, 10.1126/science.7690155.
-
(1993)
Science (80-)
, vol.261
, pp. 1411-1418
-
-
Bartel, D.P.1
Szostak, J.W.2
-
32
-
-
0032541654
-
RNA-catalysed nucleotide synthesis
-
Unrau, P.J., Bartel, D.P., RNA-catalysed nucleotide synthesis. Nature 395 (1998), 260–263, 10.1038/26193.
-
(1998)
Nature
, vol.395
, pp. 260-263
-
-
Unrau, P.J.1
Bartel, D.P.2
-
33
-
-
0034605922
-
An allosteric ribozyme regulated by doxycyline
-
Piganeau, N., Jenne, A., Thuillier, V., Famulok, M., An allosteric ribozyme regulated by doxycyline. Angew Chem Int Ed 39 (2000), 4369–4373, 10.1002/1521-3773(20001201)39:23<4369::AID-ANIE4369>3.0.CO;2-N.
-
(2000)
Angew Chem Int Ed
, vol.39
, pp. 4369-4373
-
-
Piganeau, N.1
Jenne, A.2
Thuillier, V.3
Famulok, M.4
-
34
-
-
0032491867
-
Creation of enantioselective biocatalysts for organic chemistry by in vitro evolution
-
Reetz, M.T., Zonta, A., Schimossek, K., Jaeger, K.-E., Liebeton, K., Jaeger, K.-E., Creation of enantioselective biocatalysts for organic chemistry by in vitro evolution. Angew Chem Int Ed Engl 36 (1997), 2830–2832, 10.1002/anie.199728301.
-
(1997)
Angew Chem Int Ed Engl
, vol.36
, pp. 2830-2832
-
-
Reetz, M.T.1
Zonta, A.2
Schimossek, K.3
Jaeger, K.-E.4
Liebeton, K.5
Jaeger, K.-E.6
-
35
-
-
70450223109
-
Changing the DNA recognition specificity of the EcoDam DNA-(adenine-N6)-methyltransferase by directed evolution
-
Chahar, S., Elsawy, H., Ragozin, S., Jeltsch, A., Changing the DNA recognition specificity of the EcoDam DNA-(adenine-N6)-methyltransferase by directed evolution. J Mol Biol 395 (2010), 79–88, 10.1016/j.jmb.2009.09.027.
-
(2010)
J Mol Biol
, vol.395
, pp. 79-88
-
-
Chahar, S.1
Elsawy, H.2
Ragozin, S.3
Jeltsch, A.4
-
36
-
-
0006688434
-
Simultaneous enhancement of thermostability and catalytic activity of phospholipase a 1 by evolutionary molecular engineering simultaneous enhancement of thermostability and catalytic activity of phospholipase a 1 by evolutionary molecular engineering
-
Song, J.K., Rhee, J.S., Song, J. a EK., Simultaneous enhancement of thermostability and catalytic activity of phospholipase a 1 by evolutionary molecular engineering simultaneous enhancement of thermostability and catalytic activity of phospholipase a 1 by evolutionary molecular engineering. Appl Environ Microbiol 66 (2000), 890–894, 10.1128/AEM.66.3.890-894.2000.Updated.
-
(2000)
Appl Environ Microbiol
, vol.66
, pp. 890-894
-
-
Song, J.K.1
Rhee, J.S.2
Song, J.A.E.3
-
37
-
-
34249731337
-
Effect of exchange of amino acid residues of the surface region of the PST-01 protease on its organic solvent-stability
-
Ogino, H., Uchiho, T., Doukyu, N., Yasuda, M., Ishimi, K., Ishikawa, H., Effect of exchange of amino acid residues of the surface region of the PST-01 protease on its organic solvent-stability. Biochem Biophys Res Commun 358 (2007), 1028–1033, 10.1016/j.bbrc.2007.05.047.
-
(2007)
Biochem Biophys Res Commun
, vol.358
, pp. 1028-1033
-
-
Ogino, H.1
Uchiho, T.2
Doukyu, N.3
Yasuda, M.4
Ishimi, K.5
Ishikawa, H.6
-
38
-
-
80054032745
-
Isolation of a thermostable variant of Lip2 lipase from Yarrowia lipolytica by directed evolution and deeper insight into the denaturation mechanisms involved
-
Bordes, F., Tarquis, L., Nicaud, J.M., Marty, A., Isolation of a thermostable variant of Lip2 lipase from Yarrowia lipolytica by directed evolution and deeper insight into the denaturation mechanisms involved. J Biotechnol 156 (2011), 117–124, 10.1016/j.jbiotec.2011.06.035.
-
(2011)
J Biotechnol
, vol.156
, pp. 117-124
-
-
Bordes, F.1
Tarquis, L.2
Nicaud, J.M.3
Marty, A.4
-
39
-
-
34547958191
-
Selection and evolution of enzymes from a partially randomized non-catalytic scaffold
-
Seelig, B., Szostak, J.W., Selection and evolution of enzymes from a partially randomized non-catalytic scaffold. Nat (London, U K) 448 (2007), 828–831, 10.1038/nature06032.
-
(2007)
Nat (London, U K)
, vol.448
, pp. 828-831
-
-
Seelig, B.1
Szostak, J.W.2
-
40
-
-
0036300748
-
Biodegradation of 1, 2, 3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene
-
Bosma, T., Damborský, J., Biodegradation of 1, 2, 3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene. Appl Environ Microbiol 68 (2002), 3582–3587, 10.1128/AEM.68.7.3582.
-
(2002)
Appl Environ Microbiol
, vol.68
, pp. 3582-3587
-
-
Bosma, T.1
Damborský, J.2
-
41
-
-
0036656222
-
Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis
-
Lingen, B., Grötzinger, J., Kolter, D., Kula, M.R., Pohl, M., Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis. Protein Eng 15 (2002), 585–593, 10.1093/protein/15.7.585.
-
(2002)
Protein Eng
, vol.15
, pp. 585-593
-
-
Lingen, B.1
Grötzinger, J.2
Kolter, D.3
Kula, M.R.4
Pohl, M.5
-
42
-
-
0034608808
-
A DNA enzyme with N-glycosylase activity
-
Sheppard, T.L., Ordoukhanian, P., Joyce, G.F., A DNA enzyme with N-glycosylase activity. Proc Natl Acad Sci 97 (2000), 7802–7807, 10.1073/pnas.97.14.7802.
-
(2000)
Proc Natl Acad Sci
, vol.97
, pp. 7802-7807
-
-
Sheppard, T.L.1
Ordoukhanian, P.2
Joyce, G.F.3
-
43
-
-
17844400062
-
Identification of a carotenoid oxygenase synthesizing acyclic xanthophylls: combinatorial biosynthesis and directed evolution
-
Mijts, B.N., Lee, P.C., Schmidt-Dannert, C., Identification of a carotenoid oxygenase synthesizing acyclic xanthophylls: combinatorial biosynthesis and directed evolution. Chem Biol 12 (2005), 453–460, 10.1016/j.chembiol.2005.02.010.
-
(2005)
Chem Biol
, vol.12
, pp. 453-460
-
-
Mijts, B.N.1
Lee, P.C.2
Schmidt-Dannert, C.3
-
44
-
-
33644980943
-
Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E. coli
-
Parikh, M.R., Greene, D.N., Woods, K.K., Matsumura, I., Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E. coli. Protein Eng Des Sel 19 (2006), 113–119, 10.1093/protein/gzj010.
-
(2006)
Protein Eng Des Sel
, vol.19
, pp. 113-119
-
-
Parikh, M.R.1
Greene, D.N.2
Woods, K.K.3
Matsumura, I.4
-
45
-
-
0032539818
-
Identification of an activation region in the proteasome activator REGalpha
-
Zhang, Z., Clawson, a, Realini, C., Jensen, C.C., Knowlton, J.R., Hill, C.P., et al. Identification of an activation region in the proteasome activator REGalpha. Proc Natl Acad Sci U. S. A 95 (1998), 2807–2811, 10.1073/pnas.95.6.2807.
-
(1998)
Proc Natl Acad Sci U. S. A
, vol.95
, pp. 2807-2811
-
-
Zhang, Z.1
Clawson, A.2
Realini, C.3
Jensen, C.C.4
Knowlton, J.R.5
Hill, C.P.6
-
46
-
-
0034691035
-
Systematic identification of mutations that constitutively activate the angiotensin II type 1A receptor by screening a randomly mutated cDNA library with an original pharmacological bioassay
-
Parnot, C., Bardin, S., Miserey-Lenkei, S., Guedin, D., Corvol, P., Clauser, E., Systematic identification of mutations that constitutively activate the angiotensin II type 1A receptor by screening a randomly mutated cDNA library with an original pharmacological bioassay. Proc Natl Acad Sci U. S. A 97 (2000), 7615–7620, 10.1073/pnas.110142297.
-
(2000)
Proc Natl Acad Sci U. S. A
, vol.97
, pp. 7615-7620
-
-
Parnot, C.1
Bardin, S.2
Miserey-Lenkei, S.3
Guedin, D.4
Corvol, P.5
Clauser, E.6
-
47
-
-
0029988249
-
Altered recognition mutants of the response regulator PhoB: a new genetic strategy for studying protein-protein interactions
-
Haldimann, A., Prahalad, M.K., Fisher, S.L., Kim, S.-K., Walsh, C.T., Wanner, B.L., Altered recognition mutants of the response regulator PhoB: a new genetic strategy for studying protein-protein interactions. Proc Natl Acad Sci 93 (1996), 14361–14366, 10.1073/pnas.93.25.14361.
-
(1996)
Proc Natl Acad Sci
, vol.93
, pp. 14361-14366
-
-
Haldimann, A.1
Prahalad, M.K.2
Fisher, S.L.3
Kim, S.-K.4
Walsh, C.T.5
Wanner, B.L.6
-
48
-
-
0034610373
-
Targeted modification and transportation of cellular proteins
-
Colas, P., Cohen, B., Ko Ferrigno, P., Silver, P.A., Brent, R., Targeted modification and transportation of cellular proteins. Proc Natl Acad Sci U. S. A 97 (2000), 13720–13725, 10.1073/pnas.97.25.13720.
-
(2000)
Proc Natl Acad Sci U. S. A
, vol.97
, pp. 13720-13725
-
-
Colas, P.1
Cohen, B.2
Ko Ferrigno, P.3
Silver, P.A.4
Brent, R.5
-
49
-
-
79958264719
-
GAP promoter library for fine-tuning of gene expression in Pichia pastoris
-
Qin, X., Qian, J., Yao, G., Zhuang, Y., Zhang, S., Chu, J., GAP promoter library for fine-tuning of gene expression in Pichia pastoris. Appl Environ Microbiol 77 (2011), 3600–3608, 10.1128/AEM.02843-10.
-
(2011)
Appl Environ Microbiol
, vol.77
, pp. 3600-3608
-
-
Qin, X.1
Qian, J.2
Yao, G.3
Zhuang, Y.4
Zhang, S.5
Chu, J.6
-
50
-
-
0037168510
-
Directed evolution of a genetic circuit
-
Yokobayashi, Y., Weiss, R., Arnold, F.H., Directed evolution of a genetic circuit. Proc Natl Acad Sci U. S. A 99 (2002), 16587–16591, 10.1073/pnas.252535999.
-
(2002)
Proc Natl Acad Sci U. S. A
, vol.99
, pp. 16587-16591
-
-
Yokobayashi, Y.1
Weiss, R.2
Arnold, F.H.3
-
51
-
-
0029999325
-
Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions
-
Vartanian, J.P., Henry, M., Wain-Hobson, S., Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions. Nucleic Acids Res 24 (1996), 2627–2631, 10.1093/nar/24.14.2627.
-
(1996)
Nucleic Acids Res
, vol.24
, pp. 2627-2631
-
-
Vartanian, J.P.1
Henry, M.2
Wain-Hobson, S.3
-
52
-
-
0031909113
-
Molecular evolution by staggered extension process (StEP) in vitro recombination
-
Zhao, H., Giver, L., Shao, Z., Affholter, J.A., Arnold, F.H., Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat Biotechnol 16 (1998), 258–261, 10.1038/nbt0398-258.
-
(1998)
Nat Biotechnol
, vol.16
, pp. 258-261
-
-
Zhao, H.1
Giver, L.2
Shao, Z.3
Affholter, J.A.4
Arnold, F.H.5
-
53
-
-
2342535792
-
Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution
-
Wong, T.S., Tee, K.L., Hauer, B., Schwaneberg, U., Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution. Nucleic Acids Res, 32, 2004, e26, 10.1093/nar/gnh028.
-
(2004)
Nucleic Acids Res
, vol.32
-
-
Wong, T.S.1
Tee, K.L.2
Hauer, B.3
Schwaneberg, U.4
-
54
-
-
80054894865
-
Easy two-step method for randomizing and cloning gene fragments
-
Zhang, V.Q., Hogrefe, H.H., Easy two-step method for randomizing and cloning gene fragments. Methods Mol Biol 634 (2010), 399–407, 10.1007/978-1-60761-652-8_28.
-
(2010)
Methods Mol Biol
, vol.634
, pp. 399-407
-
-
Zhang, V.Q.1
Hogrefe, H.H.2
-
55
-
-
68949161807
-
Programming cells by multiplex genome engineering and accelerated evolution
-
Wang, H.H., Isaacs, F.J., Carr, P.A., Sun, Z.Z., Xu, G., Forest, C.R., et al. Programming cells by multiplex genome engineering and accelerated evolution. Nature 460 (2009), 894–898, 10.1038/nature08187.
-
(2009)
Nature
, vol.460
, pp. 894-898
-
-
Wang, H.H.1
Isaacs, F.J.2
Carr, P.A.3
Sun, Z.Z.4
Xu, G.5
Forest, C.R.6
-
56
-
-
84919701495
-
Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA
-
Gallagher, R.R., Li, Z., Lewis, A.O., Isaacs, F.J., Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. Nat Protoc 9 (2014), 2301–2316, 10.1038/nprot.2014.082.
-
(2014)
Nat Protoc
, vol.9
, pp. 2301-2316
-
-
Gallagher, R.R.1
Li, Z.2
Lewis, A.O.3
Isaacs, F.J.4
-
57
-
-
0034705144
-
An efficient recombination system for chromosome engineering in Escherichia coli
-
Yu, D., Ellis, H.M., Lee, E.C., Jenkins, N.A., Copeland, N.G., Court, D.L., An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci U. S. A 97 (2000), 5978–5983, 10.1073/pnas.100127597.
-
(2000)
Proc Natl Acad Sci U. S. A
, vol.97
, pp. 5978-5983
-
-
Yu, D.1
Ellis, H.M.2
Lee, E.C.3
Jenkins, N.A.4
Copeland, N.G.5
Court, D.L.6
-
58
-
-
79957530428
-
-
first ed. Elsevier Inc.
-
Wang, H.H., Church, G.M., first ed. Multiplexed genome engineering and genotyping methods: applications for synthetic biology and metabolic engineering, vol. 498, 2011, Elsevier Inc., 10.1016/B978-0-12-385120-8.00018-8.
-
(2011)
Multiplexed genome engineering and genotyping methods: applications for synthetic biology and metabolic engineering
, vol.498
-
-
Wang, H.H.1
Church, G.M.2
-
59
-
-
79960502359
-
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
-
Isaacs, F.J., Carr, P.A., Wang, H.H., Lajoie, M.J., Sterling, B., Kraal, L., et al. Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science (80-) 333 (2011), 348–353, 10.1126/science.1205822.
-
(2011)
Science (80-)
, vol.333
, pp. 348-353
-
-
Isaacs, F.J.1
Carr, P.A.2
Wang, H.H.3
Lajoie, M.J.4
Sterling, B.5
Kraal, L.6
-
60
-
-
84861963767
-
Genome-scale promoter engineering by coselection MAGE
-
Wang, H.H., Kim, H., Cong, L., Jeong, J., Bang, D., Church, G.M., Genome-scale promoter engineering by coselection MAGE. Nat Methods 9 (2012), 591–593, 10.1038/nmeth.1971.
-
(2012)
Nat Methods
, vol.9
, pp. 591-593
-
-
Wang, H.H.1
Kim, H.2
Cong, L.3
Jeong, J.4
Bang, D.5
Church, G.M.6
-
61
-
-
84863328748
-
Multiplexed in vivo his-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis
-
Wang, H.H., Huang, P.Y., Xu, G., Haas, W., Marblestone, A., Li, J., et al. Multiplexed in vivo his-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis. ACS Synth Biol 1 (2012), 43–52, 10.1021/sb3000029.
-
(2012)
ACS Synth Biol
, vol.1
, pp. 43-52
-
-
Wang, H.H.1
Huang, P.Y.2
Xu, G.3
Haas, W.4
Marblestone, A.5
Li, J.6
-
62
-
-
84885791219
-
Genomically recoded organisms expand biological functions
-
Lajoie, M.J., Rovner, A.J., Goodman, D.B., Aerni, H.-R., Haimovich, A.D., Kuznetsov, G., et al. Genomically recoded organisms expand biological functions. Science (80-) 342 (2013), 357–360, 10.1126/science.1241459.
-
(2013)
Science (80-)
, vol.342
, pp. 357-360
-
-
Lajoie, M.J.1
Rovner, A.J.2
Goodman, D.B.3
Aerni, H.-R.4
Haimovich, A.D.5
Kuznetsov, G.6
-
63
-
-
84922595374
-
Recoded organisms engineered to depend on synthetic amino acids
-
Rovner, A.J., Haimovich, A.D., Katz, S.R., Li, Z., Grome, M.W., Gassaway, B.M., et al. Recoded organisms engineered to depend on synthetic amino acids. Nature 518 (2015), 89–93, 10.1038/nature14095.
-
(2015)
Nature
, vol.518
, pp. 89-93
-
-
Rovner, A.J.1
Haimovich, A.D.2
Katz, S.R.3
Li, Z.4
Grome, M.W.5
Gassaway, B.M.6
-
64
-
-
79953069562
-
Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli
-
Minty, J.J., Lesnefsky, A a, Lin, F., Chen, Y., Zaroff, T a, Veloso, A.B., et al. Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli. Microb Cell Fact, 10, 2011, 18, 10.1186/1475-2859-10-18.
-
(2011)
Microb Cell Fact
, vol.10
, pp. 18
-
-
Minty, J.J.1
Lesnefsky, A.A.2
Lin, F.3
Chen, Y.4
Zaroff, T.A.5
Veloso, A.B.6
-
65
-
-
84880200801
-
Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation
-
Binder, S., Siedler, S., Marienhagen, J., Bott, M., Eggeling, L., Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation. Nucleic Acids Res 41 (2013), 6360–6369, 10.1093/nar/gkt312.
-
(2013)
Nucleic Acids Res
, vol.41
, pp. 6360-6369
-
-
Binder, S.1
Siedler, S.2
Marienhagen, J.3
Bott, M.4
Eggeling, L.5
-
66
-
-
84890920555
-
Yeast oligo-mediated genome engineering (YOGE)
-
Dicarlo, J.E., Conley, A.J., Penttilä, M., Jä, J., Wang, H.H., Church, G.M., Yeast oligo-mediated genome engineering (YOGE). 2013, 10.1021/sb400117c.
-
(2013)
-
-
Dicarlo, J.E.1
Conley, A.J.2
Penttilä, M.3
Jä, J.4
Wang, H.H.5
Church, G.M.6
-
67
-
-
84921417685
-
Direct mutagenesis of thousands of genomic targets using microarray-derived oligonucleotides
-
Bonde, M.T., Kosuri, S., Genee, H.J., Sarup-Lytzen, K., Church, G.M., Sommer, MO a, et al. Direct mutagenesis of thousands of genomic targets using microarray-derived oligonucleotides. ACS Synth Biol, 2014, 10.1021/sb5001565.
-
(2014)
ACS Synth Biol
-
-
Bonde, M.T.1
Kosuri, S.2
Genee, H.J.3
Sarup-Lytzen, K.4
Church, G.M.5
Sommer, M.A.6
-
68
-
-
84899861853
-
Conditional DNA repair mutants enable highly precise genome engineering
-
Nyerges, Á., Csörgo, B., Nagy, I., Latinovics, D., Szamecz, B., Pósfai, G., et al. Conditional DNA repair mutants enable highly precise genome engineering. Nucleic Acids Res, 2014, 42, 10.1093/nar/gku105.
-
(2014)
Nucleic Acids Res
, pp. 42
-
-
Nyerges, Á.1
Csörgo, B.2
Nagy, I.3
Latinovics, D.4
Szamecz, B.5
Pósfai, G.6
-
69
-
-
84955464550
-
CRMAGE: CRISPR optimized MAGE recombineering
-
Ronda, C., Pedersen, L.E., Sommer, M.O.A., Nielsen, A.T., CRMAGE: CRISPR optimized MAGE recombineering. Sci Rep, 6, 2016, 19452, 10.1038/srep19452.
-
(2016)
Sci Rep
, vol.6
, pp. 19452
-
-
Ronda, C.1
Pedersen, L.E.2
Sommer, M.O.A.3
Nielsen, A.T.4
-
70
-
-
2642699794
-
Rapid and efficient site-specific mutagenesis without phenotypic selection
-
Kunkel, T.A., Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U. S. A 82 (1985), 488–492.
-
(1985)
Proc Natl Acad Sci U. S. A
, vol.82
, pp. 488-492
-
-
Kunkel, T.A.1
-
71
-
-
84871288267
-
PFunkel: efficient, expansive, user-defined mutagenesis
-
Firnberg, E., Ostermeier, M., PFunkel: efficient, expansive, user-defined mutagenesis. PLoS One, 7, 2012, e52031, 10.1371/journal.pone.0052031.
-
(2012)
PLoS One
, vol.7
-
-
Firnberg, E.1
Ostermeier, M.2
-
72
-
-
84901408859
-
A comprehensive, high-resolution map of a Gene's fitness landscape
-
Firnberg, E., Labonte, J.W., Gray, J.J., Ostermeier, M., A comprehensive, high-resolution map of a Gene's fitness landscape. Mol Biol Evol 31 (2014), 1581–1592, 10.1093/molbev/msu081.
-
(2014)
Mol Biol Evol
, vol.31
, pp. 1581-1592
-
-
Firnberg, E.1
Labonte, J.W.2
Gray, J.J.3
Ostermeier, M.4
-
73
-
-
84946098489
-
Rapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing
-
Kowalsky, C.A., Faber, M.S., Nath, A., Dann, H.E., Kelly, V.W., Liu, L., et al. Rapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing. J Biol Chem 290 (2015), 26457–26470, 10.1074/jbc.M115.676635.
-
(2015)
J Biol Chem
, vol.290
, pp. 26457-26470
-
-
Kowalsky, C.A.1
Faber, M.S.2
Nath, A.3
Dann, H.E.4
Kelly, V.W.5
Liu, L.6
-
74
-
-
84920409500
-
Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins
-
Guntas, G., Hallett, R. a., Zimmerman, S.P., Williams, T., Yumerefendi, H., Bear, J.E., et al. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci 112 (2015), 112–117, 10.1073/pnas.1417910112.
-
(2015)
Proc Natl Acad Sci
, vol.112
, pp. 112-117
-
-
Guntas, G.1
Hallett, R.A.2
Zimmerman, S.P.3
Williams, T.4
Yumerefendi, H.5
Bear, J.E.6
-
75
-
-
84994803492
-
Determination of binding affinity upon mutation for type I dockerin-cohesin complexes from C lostridium thermocellum and C lostridium cellulolyticum using deep sequencing
-
Kowalsky, C.A., Whitehead, T.A., Determination of binding affinity upon mutation for type I dockerin-cohesin complexes from C lostridium thermocellum and C lostridium cellulolyticum using deep sequencing. Proteins Struct Funct Bioinforma 84 (2016), 1914–1928, 10.1002/prot.25175.
-
(2016)
Proteins Struct Funct Bioinforma
, vol.84
, pp. 1914-1928
-
-
Kowalsky, C.A.1
Whitehead, T.A.2
-
76
-
-
84969504564
-
Shifting fitness and epistatic landscapes reflect trade-offs along an evolutionary pathway
-
Steinberg, B., Ostermeier, M., Shifting fitness and epistatic landscapes reflect trade-offs along an evolutionary pathway. J Mol Biol 428 (2016), 2730–2743, 10.1016/j.jmb.2016.04.033.
-
(2016)
J Mol Biol
, vol.428
, pp. 2730-2743
-
-
Steinberg, B.1
Ostermeier, M.2
-
77
-
-
84947968739
-
Comprehensive sequence-flux mapping of a levoglucosan utilization pathway in E. coli
-
Klesmith, J.R., Bacik, J., Michalczyk, R., Whitehead, T.A., Comprehensive sequence-flux mapping of a levoglucosan utilization pathway in E. coli. ACS Synth Biol 4 (2015), 1235–1243, 10.1021/acssynbio.5b00131.
-
(2015)
ACS Synth Biol
, vol.4
, pp. 1235-1243
-
-
Klesmith, J.R.1
Bacik, J.2
Michalczyk, R.3
Whitehead, T.A.4
-
78
-
-
84905217368
-
Deep mutational scanning: a new style of protein science
-
Fowler, D.M., Fields, S., Deep mutational scanning: a new style of protein science. Nat Methods 11 (2014), 801–807, 10.1038/nmeth.3027.
-
(2014)
Nat Methods
, vol.11
, pp. 801-807
-
-
Fowler, D.M.1
Fields, S.2
-
79
-
-
84907022693
-
Measuring the activity of protein variants on a large scale using deep mutational scanning
-
Fowler, D.M., Stephany, J.J., Fields, S., Measuring the activity of protein variants on a large scale using deep mutational scanning. Nat Protoc 9 (2014), 2267–2284, 10.1038/nprot.2014.153.
-
(2014)
Nat Protoc
, vol.9
, pp. 2267-2284
-
-
Fowler, D.M.1
Stephany, J.J.2
Fields, S.3
-
80
-
-
84923848137
-
Massively parallel single-amino-acid mutagenesis
-
Kitzman, J.O., Starita, L.M., Lo, R.S., Fields, S., Shendure, J., Massively parallel single-amino-acid mutagenesis. Nat Methods 12 (2015), 203–206, 10.1038/nmeth.3223.
-
(2015)
Nat Methods
, vol.12
, pp. 203-206
-
-
Kitzman, J.O.1
Starita, L.M.2
Lo, R.S.3
Fields, S.4
Shendure, J.5
-
81
-
-
84961794996
-
Combinatorial evolution of enzymes and synthetic pathways using one-step PCR
-
Jin, P., Kang, Z., Zhang, J., Zhang, L., Du, G., Chen, J., Combinatorial evolution of enzymes and synthetic pathways using one-step PCR. ACS Synth Biol 5 (2016), 259–268, 10.1021/acssynbio.5b00240.
-
(2016)
ACS Synth Biol
, vol.5
, pp. 259-268
-
-
Jin, P.1
Kang, Z.2
Zhang, J.3
Zhang, L.4
Du, G.5
Chen, J.6
-
82
-
-
84990878463
-
Plasmid-based one-pot saturation mutagenesis
-
Wrenbeck, E.E., Klesmith, J.R., Stapleton, J.A., Adeniran, A., Tyo, K.E.J., Whitehead, T.A., Plasmid-based one-pot saturation mutagenesis. Nat Methods 13 (2016), 928–930, 10.1038/nmeth.4029.
-
(2016)
Nat Methods
, vol.13
, pp. 928-930
-
-
Wrenbeck, E.E.1
Klesmith, J.R.2
Stapleton, J.A.3
Adeniran, A.4
Tyo, K.E.J.5
Whitehead, T.A.6
-
83
-
-
84898778301
-
A guide to genome engineering with programmable nucleases
-
Kim, H., Kim, J.S., A guide to genome engineering with programmable nucleases. Nat Rev Genet 15 (2014), 321–334, 10.1038/nrg3686.
-
(2014)
Nat Rev Genet
, vol.15
, pp. 321-334
-
-
Kim, H.1
Kim, J.S.2
-
84
-
-
54849404458
-
MMEJ repair of double-strand breaks (director's cut): deleted sequences and alternative endings
-
McVey, M., Lee, S.E., MMEJ repair of double-strand breaks (director's cut): deleted sequences and alternative endings. Trends Genet 24 (2008), 529–538, 10.1016/j.tig.2008.08.007.
-
(2008)
Trends Genet
, vol.24
, pp. 529-538
-
-
McVey, M.1
Lee, S.E.2
-
85
-
-
0026511244
-
Functional domains in Fok I restriction endonuclease
-
Li, L., Wu, L.P., Chandrasegaran, S., Functional domains in Fok I restriction endonuclease. Proc Natl Acad Sci U. S. A 89 (1992), 4275–4279, 10.1073/pnas.89.10.4275.
-
(1992)
Proc Natl Acad Sci U. S. A
, vol.89
, pp. 4275-4279
-
-
Li, L.1
Wu, L.P.2
Chandrasegaran, S.3
-
86
-
-
0030032063
-
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain
-
Kim, Y.G., Cha, J., Chandrasegaran, S., Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc Natl Acad Sci U. S. A 93 (1996), 1156–1160, 10.1073/pnas.93.3.1156.
-
(1996)
Proc Natl Acad Sci U. S. A
, vol.93
, pp. 1156-1160
-
-
Kim, Y.G.1
Cha, J.2
Chandrasegaran, S.3
-
87
-
-
0032167717
-
FokI dimerization is required for DNA cleavage
-
Bitinaite, J., Wah, D.A., Aggarwal, A.K., Schildkraut, I., FokI dimerization is required for DNA cleavage. Proc Natl Acad Sci 95 (1998), 10570–10575, 10.1073/pnas.95.18.10570.
-
(1998)
Proc Natl Acad Sci
, vol.95
, pp. 10570-10575
-
-
Bitinaite, J.1
Wah, D.A.2
Aggarwal, A.K.3
Schildkraut, I.4
-
88
-
-
80051535219
-
Genome engineering with zinc-finger nucleases
-
Carroll, D., Genome engineering with zinc-finger nucleases. Genetics 188 (2011), 773–782, 10.1534/genetics.111.131433.
-
(2011)
Genetics
, vol.188
, pp. 773-782
-
-
Carroll, D.1
-
89
-
-
84960832490
-
Origins of programmable nucleases for genome engineering
-
Carroll, D., Origins of programmable nucleases for genome engineering. J Mol Biol 428 (2016), 963–989, 10.1016/j.jmb.2015.10.014.
-
(2016)
J Mol Biol
, vol.428
, pp. 963-989
-
-
Carroll, D.1
-
90
-
-
42949083192
-
Unexpected failure rates for modular assembly of engineered zinc fingers
-
Ramirez, C.L., Foley, J.E., Wright, D a, Müller-Lerch, F., Rahman, S.H., Cornu, T.I., et al. Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Methods 5 (2008), 374–375, 10.1038/nmeth0508-374.
-
(2008)
Nat Methods
, vol.5
, pp. 374-375
-
-
Ramirez, C.L.1
Foley, J.E.2
Wright, D.A.3
Müller-Lerch, F.4
Rahman, S.H.5
Cornu, T.I.6
-
91
-
-
38849103093
-
DNA-binding specificity is a major determinant of the activity and toxicity of zinc-finger nucleases
-
Cornu, T.I., Thibodeau-Beganny, S., Guhl, E., Alwin, S., Eichtinger, M., Joung, J.K., et al. DNA-binding specificity is a major determinant of the activity and toxicity of zinc-finger nucleases. Mol Ther 16 (2008), 352–358, 10.1038/sj.mt.6300357.
-
(2008)
Mol Ther
, vol.16
, pp. 352-358
-
-
Cornu, T.I.1
Thibodeau-Beganny, S.2
Guhl, E.3
Alwin, S.4
Eichtinger, M.5
Joung, J.K.6
-
92
-
-
0034930363
-
A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter
-
Isalan, M., Klug, A., Choo, Y., A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter. Nat Biotechnol 19 (2001), 656–660, 10.1038/90264.
-
(2001)
Nat Biotechnol
, vol.19
, pp. 656-660
-
-
Isalan, M.1
Klug, A.2
Choo, Y.3
-
93
-
-
0031053362
-
A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
-
Greisman, H.A., Pabo, C.O., A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites. Science 275 (1997), 657–661.
-
(1997)
Science
, vol.275
, pp. 657-661
-
-
Greisman, H.A.1
Pabo, C.O.2
-
94
-
-
77957005768
-
Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases
-
Meyer, M., de Angelis, M.H., Wurst, W., Kühn, R., Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases. Proc Natl Acad Sci U. S. A 107 (2010), 15022–15026, 10.1073/pnas.1009424107.
-
(2010)
Proc Natl Acad Sci U. S. A
, vol.107
, pp. 15022-15026
-
-
Meyer, M.1
de Angelis, M.H.2
Wurst, W.3
Kühn, R.4
-
95
-
-
66249147273
-
Precise genome modification in the crop species Zea mays using zinc-finger nucleases
-
Shukla, V.K., Doyon, Y., Miller, J.C., DeKelver, R.C., Moehle, E a, Worden, S.E., et al. Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature 459 (2009), 437–441, 10.1038/nature07992.
-
(2009)
Nature
, vol.459
, pp. 437-441
-
-
Shukla, V.K.1
Doyon, Y.2
Miller, J.C.3
DeKelver, R.C.4
Moehle, E.A.5
Worden, S.E.6
-
96
-
-
77956126894
-
Xanthomonas AvrBs3 family-type III effectors: discovery and function
-
Boch, J., Bonas, U., Xanthomonas AvrBs3 family-type III effectors: discovery and function. Annu Rev Phytopathol 48 (2010), 419–436, 10.1146/annurev-phyto-080508-081936.
-
(2010)
Annu Rev Phytopathol
, vol.48
, pp. 419-436
-
-
Boch, J.1
Bonas, U.2
-
97
-
-
79551685675
-
A TALE nuclease architecture for efficient genome editing
-
Miller, J.C., Tan, S., Qiao, G., Barlow, K.A., Wang, J., Xia, D.F., et al. A TALE nuclease architecture for efficient genome editing. Nat Biotechnol 29 (2011), 143–148, 10.1038/nbt.1755.
-
(2011)
Nat Biotechnol
, vol.29
, pp. 143-148
-
-
Miller, J.C.1
Tan, S.2
Qiao, G.3
Barlow, K.A.4
Wang, J.5
Xia, D.F.6
-
98
-
-
78650739853
-
Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors
-
Morbitzer, R., Römer, P., Boch, J., Lahaye, T., Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors. Proc Natl Acad Sci 107 (2010), 21617–21622, 10.1073/pnas.1013133107.
-
(2010)
Proc Natl Acad Sci
, vol.107
, pp. 21617-21622
-
-
Morbitzer, R.1
Römer, P.2
Boch, J.3
Lahaye, T.4
-
99
-
-
84876389220
-
Differential integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells
-
Holkers, M., Maggio, I., Liu, J., Janssen, J.M., Miselli, F., Mussolino, C., et al. Differential integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells. Nucleic Acids Res, 2013, 41, 10.1093/nar/gks1446.
-
(2013)
Nucleic Acids Res
, pp. 41
-
-
Holkers, M.1
Maggio, I.2
Liu, J.3
Janssen, J.M.4
Miselli, F.5
Mussolino, C.6
-
100
-
-
80053217562
-
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
-
Cermak, T., Doyle, E.L., Christian, M., Wang, L., Zhang, Y., Schmidt, C., et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res, 39, 2011, 7879, 10.1093/nar/gkr739.
-
(2011)
Nucleic Acids Res
, vol.39
, pp. 7879
-
-
Cermak, T.1
Doyle, E.L.2
Christian, M.3
Wang, L.4
Zhang, Y.5
Schmidt, C.6
-
101
-
-
84860747716
-
FLASH assembly of TALENs for high-throughput genome editing
-
Reyon, D., Tsai, S.Q., Khayter, C., Foden, J.A., Sander, J.D., Joung, J.K., FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol 30 (2012), 460–465, 10.1038/nbt.2170.
-
(2012)
Nat Biotechnol
, vol.30
, pp. 460-465
-
-
Reyon, D.1
Tsai, S.Q.2
Khayter, C.3
Foden, J.A.4
Sander, J.D.5
Joung, J.K.6
-
102
-
-
84872203111
-
A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes
-
Schmid-Burgk, J.L., Schmidt, T., Kaiser, V., Höning, K., Hornung, V., A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes. Nat Biotechnol 31 (2013), 76–81, 10.1038/nbt.2460.
-
(2013)
Nat Biotechnol
, vol.31
, pp. 76-81
-
-
Schmid-Burgk, J.L.1
Schmidt, T.2
Kaiser, V.3
Höning, K.4
Hornung, V.5
-
103
-
-
80053039555
-
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
-
Mussolino, C., Morbitzer, R., Lütge, F., Dannemann, N., Lahaye, T., Cathomen, T., A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res 39 (2011), 9283–9293, 10.1093/nar/gkr597.
-
(2011)
Nucleic Acids Res
, vol.39
, pp. 9283-9293
-
-
Mussolino, C.1
Morbitzer, R.2
Lütge, F.3
Dannemann, N.4
Lahaye, T.5
Cathomen, T.6
-
104
-
-
84868342049
-
In vivo genome editing using a high-efficiency TALEN system
-
Bedell, V.M., Wang, Y., Campbell, J.M., Poshusta, T.L., Starker, C.G., Krug, R.G., et al. In vivo genome editing using a high-efficiency TALEN system. Nature 491 (2012), 114–118, 10.1038/nature11537.
-
(2012)
Nature
, vol.491
, pp. 114-118
-
-
Bedell, V.M.1
Wang, Y.2
Campbell, J.M.3
Poshusta, T.L.4
Starker, C.G.5
Krug, R.G.6
-
105
-
-
84944449180
-
An updated evolutionary classification of CRISPR-Cas systems
-
Makarova, K.S., Wolf, Y.I., Alkhnbashi, O.S., Costa, F., Shah, S.A., Saunders, S.J., et al. An updated evolutionary classification of CRISPR-Cas systems. Nat Rev Microbiol 13 (2015), 722–736, 10.1038/nrmicro3569.
-
(2015)
Nat Rev Microbiol
, vol.13
, pp. 722-736
-
-
Makarova, K.S.1
Wolf, Y.I.2
Alkhnbashi, O.S.3
Costa, F.4
Shah, S.A.5
Saunders, S.J.6
-
106
-
-
59849096566
-
The red queen and the court jester: species diversity and the role of biotic and abiotic factors through time
-
Benton, M.J., The red queen and the court jester: species diversity and the role of biotic and abiotic factors through time. Science (80-) 323 (2009), 728–732, 10.1126/science.1157719.
-
(2009)
Science (80-)
, vol.323
, pp. 728-732
-
-
Benton, M.J.1
-
107
-
-
85017393597
-
CRISPR-Cas: adapting to change
-
Jackson, S.A., McKenzie, R.E., Fagerlund, R.D., Kieper, S.N., Fineran, P.C., Brouns, S.J.J., CRISPR-Cas: adapting to change. Science (80-), 2017, 356, 10.1126/science.aal5056.
-
(2017)
Science (80-)
, pp. 356
-
-
Jackson, S.A.1
McKenzie, R.E.2
Fagerlund, R.D.3
Kieper, S.N.4
Fineran, P.C.5
Brouns, S.J.J.6
-
108
-
-
79953250082
-
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
-
Deltcheva, E., Chylinski, K., Sharma, C.M., Gonzales, K., Chao, Y., Pirzada, Z.A., et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 471 (2011), 602–607, 10.1038/nature09886.
-
(2011)
Nature
, vol.471
, pp. 602-607
-
-
Deltcheva, E.1
Chylinski, K.2
Sharma, C.M.3
Gonzales, K.4
Chao, Y.5
Pirzada, Z.A.6
-
109
-
-
84974717567
-
CRISPR/Cas9 in genome editing and beyond
-
Wang, H., La Russa, M., Qi, L.S., CRISPR/Cas9 in genome editing and beyond. Annu Rev Biochem 85 (2016), 227–264, 10.1146/annurev-biochem-060815-014607.
-
(2016)
Annu Rev Biochem
, vol.85
, pp. 227-264
-
-
Wang, H.1
La Russa, M.2
Qi, L.S.3
-
110
-
-
84947930171
-
Exploiting CRISPR-Cas immune systems for genome editing in bacteria
-
Barrangou, R., van Pijkeren, J.P., Exploiting CRISPR-Cas immune systems for genome editing in bacteria. Curr Opin Biotechnol 37 (2016), 61–68, 10.1016/j.copbio.2015.10.003.
-
(2016)
Curr Opin Biotechnol
, vol.37
, pp. 61-68
-
-
Barrangou, R.1
van Pijkeren, J.P.2
-
111
-
-
84865070369
-
A programmable dual-RNA – guided DNA endonuclease in adaptive bacterial immunity
-
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., Charpentier, E., A programmable dual-RNA – guided DNA endonuclease in adaptive bacterial immunity. Science 337 (2012), 816–822, 10.1126/science.1225829.
-
(2012)
Science
, vol.337
, pp. 816-822
-
-
Jinek, M.1
Chylinski, K.2
Fonfara, I.3
Hauer, M.4
Doudna, J.A.5
Charpentier, E.6
-
112
-
-
84873729095
-
Multiplex genome engineering using CRISPR/cas systems
-
Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., et al. Multiplex genome engineering using CRISPR/cas systems. Science (80-) 339 (2013), 819–823, 10.1126/science.1231143.
-
(2013)
Science (80-)
, vol.339
, pp. 819-823
-
-
Cong, L.1
Ran, F.A.2
Cox, D.3
Lin, S.4
Barretto, R.5
Habib, N.6
-
113
-
-
84923021733
-
Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae
-
Jakočinas, T., Bonde, I., Herrgård, M., Harrison, S.J., Kristensen, M., Pedersen, L.E., et al. Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae. Metab Eng 28 (2015), 213–222, 10.1016/j.ymben.2015.01.008.
-
(2015)
Metab Eng
, vol.28
, pp. 213-222
-
-
Jakočinas, T.1
Bonde, I.2
Herrgård, M.3
Harrison, S.J.4
Kristensen, M.5
Pedersen, L.E.6
-
114
-
-
84874608929
-
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
-
Jiang, W., Bikard, D., Cox, D., Zhang, F., Marraffini, La, RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol 31 (2013), 233–239, 10.1038/nbt.2508.
-
(2013)
Nat Biotechnol
, vol.31
, pp. 233-239
-
-
Jiang, W.1
Bikard, D.2
Cox, D.3
Zhang, F.4
Marraffini, L.5
-
115
-
-
84942368560
-
Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR
-
Tsai, C.S., Kong II, Lesmana, A., Million, G., Zhang, G.C., Kim, S.R., et al. Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR. Biotechnol Bioeng 112 (2015), 2406–2411, 10.1002/bit.25632.
-
(2015)
Biotechnol Bioeng
, vol.112
, pp. 2406-2411
-
-
Tsai, C.S.1
Kong2
Lesmana, A.3
Million, G.4
Zhang, G.C.5
Kim, S.R.6
-
116
-
-
84947279264
-
A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae
-
Shi, S., Liang, Y., Zhang, M.M., Ang, E.L., Zhao, H., A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae. Metab Eng 33 (2016), 19–27, 10.1016/j.ymben.2015.10.011.
-
(2016)
Metab Eng
, vol.33
, pp. 19-27
-
-
Shi, S.1
Liang, Y.2
Zhang, M.M.3
Ang, E.L.4
Zhao, H.5
-
117
-
-
84933569948
-
CasEMBLR: cas9-facilitated multiloci genomic integration of in vivo assembled DNA parts in Saccharomyces cerevisiae
-
Jakočinas, T., Rajkumar, A.S., Zhang, J., Arsovska, D., Rodriguez, A., ille, Jendresen CB., et al. CasEMBLR: cas9-facilitated multiloci genomic integration of in vivo assembled DNA parts in Saccharomyces cerevisiae. ACS Synth Biol 4 (2015), 1226–1234, 10.1021/acssynbio.5b00007.
-
(2015)
ACS Synth Biol
, vol.4
, pp. 1226-1234
-
-
Jakočinas, T.1
Rajkumar, A.S.2
Zhang, J.3
Arsovska, D.4
Rodriguez, A.5
ille, J.C.6
-
118
-
-
84937538704
-
Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing
-
Li, Y., Lin, Z., Huang, C., Zhang, Y., Wang, Z., Tang, Y Jie, et al. Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab Eng 31 (2015), 13–21, 10.1016/j.ymben.2015.06.006.
-
(2015)
Metab Eng
, vol.31
, pp. 13-21
-
-
Li, Y.1
Lin, Z.2
Huang, C.3
Zhang, Y.4
Wang, Z.5
Tang, Y.J.6
-
119
-
-
84911871184
-
Selection of chromosomal DNA libraries using a multiplex CRISPR system
-
Ryan, O.W., Skerker, J.M., Maurer, M.J., Li, X., Tsai, J.C., Poddar, S., et al. Selection of chromosomal DNA libraries using a multiplex CRISPR system. Elife 3 (2014), 1–15, 10.7554/eLife.03703.
-
(2014)
Elife
, vol.3
, pp. 1-15
-
-
Ryan, O.W.1
Skerker, J.M.2
Maurer, M.J.3
Li, X.4
Tsai, J.C.5
Poddar, S.6
-
120
-
-
78651341148
-
Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases
-
Gupta, A., Meng, X., Zhu, L.J., Lawson, N.D., Wolfe, S.A., Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res 39 (2011), 381–392, 10.1093/nar/gkq787.
-
(2011)
Nucleic Acids Res
, vol.39
, pp. 381-392
-
-
Gupta, A.1
Meng, X.2
Zhu, L.J.3
Lawson, N.D.4
Wolfe, S.A.5
-
121
-
-
80052766645
-
An unbiased genome-wide analysis of zinc-finger nuclease specificity
-
Gabriel, R., Lombardo, A., Arens, A., Miller, J.C., Genovese, P., Kaeppel, C., et al. An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol 29 (2011), 816–823, 10.1038/nbt.1948.
-
(2011)
Nat Biotechnol
, vol.29
, pp. 816-823
-
-
Gabriel, R.1
Lombardo, A.2
Arens, A.3
Miller, J.C.4
Genovese, P.5
Kaeppel, C.6
-
122
-
-
84878580410
-
TALEN-based gene correction for epidermolysis bullosa
-
Osborn, M.J., Starker, C.G., McElroy, A.N., Webber, B.R., Riddle, M.J., Xia, L., et al. TALEN-based gene correction for epidermolysis bullosa. Mol Ther 21 (2013), 1151–1159, 10.1038/mt.2013.56.
-
(2013)
Mol Ther
, vol.21
, pp. 1151-1159
-
-
Osborn, M.J.1
Starker, C.G.2
McElroy, A.N.3
Webber, B.R.4
Riddle, M.J.5
Xia, L.6
-
123
-
-
79960836456
-
Genetic engineering of human pluripotent cells using TALE nucleases
-
Hockemeyer, D., Wang, H., Kiani, S., Lai, C.S., Gao, Q., Cassady, J.P., et al. Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol 29 (2011), 731–734, 10.1038/nbt.1927.
-
(2011)
Nat Biotechnol
, vol.29
, pp. 731-734
-
-
Hockemeyer, D.1
Wang, H.2
Kiani, S.3
Lai, C.S.4
Gao, Q.5
Cassady, J.P.6
-
124
-
-
84884155038
-
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
-
Pattanayak, V., Lin, S., Guilinger, J.P., Ma, E., Doudna, J a, Liu, D.R., High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol 31 (2013), 839–843, 10.1038/nbt.2673.
-
(2013)
Nat Biotechnol
, vol.31
, pp. 839-843
-
-
Pattanayak, V.1
Lin, S.2
Guilinger, J.P.3
Ma, E.4
Doudna, J.A.5
Liu, D.R.6
-
125
-
-
84880570576
-
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
-
Fu, Y., Foden, J a, Khayter, C., Maeder, M.L., Reyon, D., Joung, J.K., et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol 31 (2013), 822–826, 10.1038/nbt.2623.
-
(2013)
Nat Biotechnol
, vol.31
, pp. 822-826
-
-
Fu, Y.1
Foden, J.A.2
Khayter, C.3
Maeder, M.L.4
Reyon, D.5
Joung, J.K.6
-
126
-
-
85047227706
-
Strategies to determine off-target effects of engineered nucleases
-
T. Cathomen M. Hirsch M. Porteus Springer New York New York, NY
-
Fine, E.J., Cradick, T.J., Bao, G., Strategies to determine off-target effects of engineered nucleases. Cathomen, T., Hirsch, M., Porteus, M., (eds.) Genome Ed. Next step gene ther., 2016, Springer New York, New York, NY, 187–222, 10.1007/978-1-4939-3509-3_11.
-
(2016)
Genome Ed. Next step gene ther.
, pp. 187-222
-
-
Fine, E.J.1
Cradick, T.J.2
Bao, G.3
-
127
-
-
34447323568
-
Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases
-
Szczepek, M., Brondani, V., Büchel, J., Serrano, L., Segal, D.J., Cathomen, T., Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases. Nat Biotechnol 25 (2007), 786–793, 10.1038/nbt1317.
-
(2007)
Nat Biotechnol
, vol.25
, pp. 786-793
-
-
Szczepek, M.1
Brondani, V.2
Büchel, J.3
Serrano, L.4
Segal, D.J.5
Cathomen, T.6
-
128
-
-
78650912707
-
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures
-
Doyon, Y., Vo, T.D., Mendel, M.C., Greenberg, S.G., Wang, J., Xia, D.F., et al. Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Meth 8 (2011), 74–79, 10.1038/nmeth.1539.
-
(2011)
Nat Meth
, vol.8
, pp. 74-79
-
-
Doyon, Y.1
Vo, T.D.2
Mendel, M.C.3
Greenberg, S.G.4
Wang, J.5
Xia, D.F.6
-
129
-
-
84952636633
-
Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
-
Dang, Y., Jia, G., Choi, J., Ma, H., Anaya, E., Ye, C., et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome Biol, 16, 2015, 280, 10.1186/s13059-015-0846-3.
-
(2015)
Genome Biol
, vol.16
, pp. 280
-
-
Dang, Y.1
Jia, G.2
Choi, J.3
Ma, H.4
Anaya, E.5
Ye, C.6
-
130
-
-
84957605863
-
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
-
Doench, J.G., Fusi, N., Sullender, M., Hegde, M., Vaimberg, E.W., Donovan, K.F., et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol 34 (2016), 1–12, 10.1038/nbt.3437.
-
(2016)
Nat Biotechnol
, vol.34
, pp. 1-12
-
-
Doench, J.G.1
Fusi, N.2
Sullender, M.3
Hegde, M.4
Vaimberg, E.W.5
Donovan, K.F.6
-
131
-
-
84997848522
-
In silico meets in vivo: towards computational CRISPR-based sgRNA design
-
Chuai, G Hui, Wang, Q.L., Liu, Q., In silico meets in vivo: towards computational CRISPR-based sgRNA design. Trends Biotechnol 35 (2016), 12–21, 10.1016/j.tibtech.2016.06.008.
-
(2016)
Trends Biotechnol
, vol.35
, pp. 12-21
-
-
Chuai, G.H.1
Wang, Q.L.2
Liu, Q.3
-
132
-
-
85019592417
-
sgRNA Scorer 2.0 – a species independent model to predict CRISPR/Cas9 activity
-
acssynbio.6b00343
-
Chari, R., Yeo, N.C., Chavez, A., Church, G.M., sgRNA Scorer 2.0 – a species independent model to predict CRISPR/Cas9 activity. ACS Synth Biol, 2017, 10.1021/acssynbio.6b00343 acssynbio.6b00343.
-
(2017)
ACS Synth Biol
-
-
Chari, R.1
Yeo, N.C.2
Chavez, A.3
Church, G.M.4
-
133
-
-
84963941043
-
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
-
Kleinstiver, B.P., Pattanayak, V., Prew, M.S., Tsai, S.Q., Nguyen, N.T., Zheng, Z., et al. High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects. Nature 529 (2016), 490–495, 10.1038/nature16526.
-
(2016)
Nature
, vol.529
, pp. 490-495
-
-
Kleinstiver, B.P.1
Pattanayak, V.2
Prew, M.S.3
Tsai, S.Q.4
Nguyen, N.T.5
Zheng, Z.6
-
134
-
-
84884288934
-
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
-
Ran, F.A., Hsu, P.D., Lin, C.-Y., Gootenberg, J.S., Konermann, S., Trevino, A.E., et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154 (2013), 1380–1389, 10.1016/j.cell.2013.08.021.
-
(2013)
Cell
, vol.154
, pp. 1380-1389
-
-
Ran, F.A.1
Hsu, P.D.2
Lin, C.-Y.3
Gootenberg, J.S.4
Konermann, S.5
Trevino, A.E.6
-
135
-
-
84876556023
-
Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats
-
Gabsalilow, L., Schierling, B., Friedhoff, P., Pingoud, A., Wende, W., Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats. Nucleic Acids Res, 41, 2013, e83, 10.1093/nar/gkt080.
-
(2013)
Nucleic Acids Res
, vol.41
, pp. e83
-
-
Gabsalilow, L.1
Schierling, B.2
Friedhoff, P.3
Pingoud, A.4
Wende, W.5
-
136
-
-
84861960685
-
Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects
-
Ramirez, C.L., Certo, M.T., Mussolino, C., Goodwin, M.J., Cradick, T.J., McCaffrey, A.P., et al. Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects. Nucleic Acids Res 40 (2012), 5560–5568, 10.1093/nar/gks179.
-
(2012)
Nucleic Acids Res
, vol.40
, pp. 5560-5568
-
-
Ramirez, C.L.1
Certo, M.T.2
Mussolino, C.3
Goodwin, M.J.4
Cradick, T.J.5
McCaffrey, A.P.6
-
137
-
-
0034323194
-
Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains
-
Fabret, C., Poncet, S., Danielsen, S., Borchert, T.V., Ehrlich, S.D., Jannière, L., Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains. Nucleic Acids Res, 28, 2000, E95.
-
(2000)
Nucleic Acids Res
, vol.28
, pp. E95
-
-
Fabret, C.1
Poncet, S.2
Danielsen, S.3
Borchert, T.V.4
Ehrlich, S.D.5
Jannière, L.6
-
138
-
-
0015184718
-
Slow joining of newly replicated DNA chains in DNA polymerase I-Deficient Escherichia coli mutants
-
Okazaki, R., Arisawa, M., Sugino, A., Slow joining of newly replicated DNA chains in DNA polymerase I-Deficient Escherichia coli mutants. Proc Natl Acad Sci 68 (1971), 2954–2957.
-
(1971)
Proc Natl Acad Sci
, vol.68
, pp. 2954-2957
-
-
Okazaki, R.1
Arisawa, M.2
Sugino, A.3
-
139
-
-
0015344221
-
Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli
-
Cooper, P.K., Hanawalt, P.C., Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli. Proc Natl Acad Sci 69 (1972), 1156–1160.
-
(1972)
Proc Natl Acad Sci
, vol.69
, pp. 1156-1160
-
-
Cooper, P.K.1
Hanawalt, P.C.2
-
140
-
-
0031811125
-
Replication and control of circular bacterial plasmids
-
doi:1092-2172/98/$04.0010
-
del Solar, G., Giraldo, R., Ruiz-Echevarría, M.J., Espinosa, M., Díaz-Orejas, R., Replication and control of circular bacterial plasmids. Microbiol Mol Biol Rev 62 (1998), 434–464 doi:1092-2172/98/$04.0010.
-
(1998)
Microbiol Mol Biol Rev
, vol.62
, pp. 434-464
-
-
del Solar, G.1
Giraldo, R.2
Ruiz-Echevarría, M.J.3
Espinosa, M.4
Díaz-Orejas, R.5
-
141
-
-
80052512523
-
Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication
-
Allen, J.M., Simcha, D.M., Ericson, N.G., Alexander, D.L., Marquette, J.T., Van Biber, B.P., et al. Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication. Nucleic Acids Res 39 (2011), 7020–7033, 10.1093/nar/gkr157.
-
(2011)
Nucleic Acids Res
, vol.39
, pp. 7020-7033
-
-
Allen, J.M.1
Simcha, D.M.2
Ericson, N.G.3
Alexander, D.L.4
Marquette, J.T.5
Van Biber, B.P.6
-
142
-
-
0043194096
-
Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I
-
Camps, M., Naukkarinen, J., Johnson, B.P., Loeb, L.A., Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I. Proc Natl Acad Sci 100 (2003), 9727–9732, 10.1073/pnas.1333928100.
-
(2003)
Proc Natl Acad Sci
, vol.100
, pp. 9727-9732
-
-
Camps, M.1
Naukkarinen, J.2
Johnson, B.P.3
Loeb, L.A.4
-
143
-
-
0141992704
-
Use of Pol I-Deficient E. coli for functional complementation of DNA polymerase
-
F.H. Arnold G. Georgiou Humana Press Totowa, NJ
-
Camps, M., Loeb, L.A., Use of Pol I-Deficient E. coli for functional complementation of DNA polymerase. Arnold, F.H., Georgiou, G., (eds.) Dir. Enzym. Evol. Screen. Sel. Methods, 2003, Humana Press, Totowa, NJ, 11–18, 10.1385/1-59259-396-8:11.
-
(2003)
Dir. Enzym. Evol. Screen. Sel. Methods
, pp. 11-18
-
-
Camps, M.1
Loeb, L.A.2
-
144
-
-
0035861641
-
In vivo mutagenesis by Escherichia coliDNA polymerase I: ILE709 in MOTIF a functions in base selection
-
Shinkai, A., Loeb, L.A., In vivo mutagenesis by Escherichia coliDNA polymerase I: ILE709 in MOTIF a functions in base selection. J Biol Chem 276 (2001), 46759–46764, 10.1074/jbc.M104780200.
-
(2001)
J Biol Chem
, vol.276
, pp. 46759-46764
-
-
Shinkai, A.1
Loeb, L.A.2
-
145
-
-
84894055645
-
An orthogonal DNA replication system in yeast
-
Ravikumar, A., Arrieta, A., Liu, C.C., An orthogonal DNA replication system in yeast. Nat Chem Biol 10 (2014), 175–177, 10.1038/nchembio.1439.
-
(2014)
Nat Chem Biol
, vol.10
, pp. 175-177
-
-
Ravikumar, A.1
Arrieta, A.2
Liu, C.C.3
-
146
-
-
0033287841
-
Mechanisms of initiation of linear DNA replication in prokaryotes. Genet. Eng. (N. Y)
-
Springer US Boston, MA
-
Salas, M., Mechanisms of initiation of linear DNA replication in prokaryotes. Genet. Eng. (N. Y). 1999, Springer US, Boston, MA, 159–171, 10.1007/978-1-4615-4707-5_8.
-
(1999)
, pp. 159-171
-
-
Salas, M.1
-
147
-
-
58149383679
-
In vivo evolution of butane oxidation by terminal alkane hydroxylases AlkB and CYP153A6
-
Koch, D.J., Chen, M.M., Van Beilen, J.B., Arnold, F.H., In vivo evolution of butane oxidation by terminal alkane hydroxylases AlkB and CYP153A6. Appl Environ Microbiol 75 (2009), 337–344, 10.1128/AEM.01758-08.
-
(2009)
Appl Environ Microbiol
, vol.75
, pp. 337-344
-
-
Koch, D.J.1
Chen, M.M.2
Van Beilen, J.B.3
Arnold, F.H.4
-
148
-
-
79955541476
-
Synthetic inter-species cooperation of host and virus for targeted genetic evolution
-
Na, D., Lee, S., Yi, G.S., Lee, D., Synthetic inter-species cooperation of host and virus for targeted genetic evolution. J Biotechnol 153 (2011), 35–41, 10.1016/j.jbiotec.2011.03.004.
-
(2011)
J Biotechnol
, vol.153
, pp. 35-41
-
-
Na, D.1
Lee, S.2
Yi, G.S.3
Lee, D.4
-
149
-
-
79958717118
-
The biochemistry and fidelity of synthesis by the apicoplast genome replication DNA polymerase Pfprex from the malaria parasite Plasmodium falciparum
-
Kennedy, S.R., Chen, C.Y., Schmitt, M.W., Bower, C.N., Loeb, L.A., The biochemistry and fidelity of synthesis by the apicoplast genome replication DNA polymerase Pfprex from the malaria parasite Plasmodium falciparum. J Mol Biol 410 (2011), 27–38, 10.1016/j.jmb.2011.04.071.
-
(2011)
J Mol Biol
, vol.410
, pp. 27-38
-
-
Kennedy, S.R.1
Chen, C.Y.2
Schmitt, M.W.3
Bower, C.N.4
Loeb, L.A.5
-
150
-
-
79959751796
-
Directed evolution of an error-prone T7 DNA polymerase that attenuates viral replication
-
Söte, S., Kleine, S., Schlicke, M., Brakmann, S., Directed evolution of an error-prone T7 DNA polymerase that attenuates viral replication. ChemBioChem 12 (2011), 1551–1558, 10.1002/cbic.201000799.
-
(2011)
ChemBioChem
, vol.12
, pp. 1551-1558
-
-
Söte, S.1
Kleine, S.2
Schlicke, M.3
Brakmann, S.4
-
151
-
-
84905376865
-
A “resource allocator” for transcription based on a highly fragmented T7 RNA polymerase
-
Segall-Shapiro, T.H., Meyer, A.J., Ellington, A.D., Sontag, E.D., Voigt, Ca, A “resource allocator” for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol, 10, 2014, 742, 10.15252/msb.20145299.
-
(2014)
Mol Syst Biol
, vol.10
, pp. 742
-
-
Segall-Shapiro, T.H.1
Meyer, A.J.2
Ellington, A.D.3
Sontag, E.D.4
Voigt, C.5
-
152
-
-
84925283926
-
Silver P a. Using synthetic RNAs as scaffolds and regulators
-
Myhrvold, C., Silver P a. Using synthetic RNAs as scaffolds and regulators. Nat Struct Mol Biol 22 (2015), 8–10, 10.1038/nsmb.2944.
-
(2015)
Nat Struct Mol Biol
, vol.22
, pp. 8-10
-
-
Myhrvold, C.1
-
153
-
-
84929336656
-
Biocontainment through reengineered genetic codes
-
Ravikumar, A., Liu, C.C., Biocontainment through reengineered genetic codes. ChemBioChem 16 (2015), 1149–1151, 10.1002/cbic.201500157.
-
(2015)
ChemBioChem
, vol.16
, pp. 1149-1151
-
-
Ravikumar, A.1
Liu, C.C.2
-
154
-
-
85013498519
-
Synthetic biology approaches to biological containment: pre-emptively tackling potential risks
-
Torres, L., Kruger, A., Csibra, E., Gianni, E., Pinheiro, V.B., Synthetic biology approaches to biological containment: pre-emptively tackling potential risks. Essays Biochem 60 (2016), 393–410, 10.1042/EBC20160013.
-
(2016)
Essays Biochem
, vol.60
, pp. 393-410
-
-
Torres, L.1
Kruger, A.2
Csibra, E.3
Gianni, E.4
Pinheiro, V.B.5
-
155
-
-
0019370839
-
Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKll and pGK12, from kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion
-
Gunge, N., Sakaguchi, K., Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKll and pGK12, from kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion. J Bacteriol 147 (1981), 155–160.
-
(1981)
J Bacteriol
, vol.147
, pp. 155-160
-
-
Gunge, N.1
Sakaguchi, K.2
-
156
-
-
84877280637
-
Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM
-
Finney-Manchester, S.P., Maheshri, N., Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM. Nucleic Acids Res, 41, 2013, e99, 10.1093/nar/gkt150.
-
(2013)
Nucleic Acids Res
, vol.41
, pp. e99
-
-
Finney-Manchester, S.P.1
Maheshri, N.2
-
157
-
-
1642411206
-
Dissecting the broad substrate specificity of human 3-methyladenine-DNA glycosylase
-
O'Brien, P.J., Ellenberger, T., Dissecting the broad substrate specificity of human 3-methyladenine-DNA glycosylase. J Biol Chem 279 (2004), 9750–9757, 10.1074/jbc.M312232200.
-
(2004)
J Biol Chem
, vol.279
, pp. 9750-9757
-
-
O'Brien, P.J.1
Ellenberger, T.2
-
158
-
-
0032544037
-
Generation of a strong mutator phenotype in yeast by imbalanced base excision repair
-
Glassner, B.J., Rasmussen, L.J., Najarian, M.T., Posnick, L.M., Samson, L.D., Generation of a strong mutator phenotype in yeast by imbalanced base excision repair. Proc Natl Acad Sci U. S. A 95 (1998), 9997–10002, 10.1073/pnas.95.17.9997.
-
(1998)
Proc Natl Acad Sci U. S. A
, vol.95
, pp. 9997-10002
-
-
Glassner, B.J.1
Rasmussen, L.J.2
Najarian, M.T.3
Posnick, L.M.4
Samson, L.D.5
-
159
-
-
84992075174
-
In vivo continuous evolution of genes and pathways in yeast
-
Crook, N., Abatemarco, J., Sun, J., Wagner, J.M., Schmitz, A., Alper, H.S., et al. In vivo continuous evolution of genes and pathways in yeast. Nat Commun, 7, 2016, 13051, 10.1038/ncomms13051.
-
(2016)
Nat Commun
, vol.7
, pp. 13051
-
-
Crook, N.1
Abatemarco, J.2
Sun, J.3
Wagner, J.M.4
Schmitz, A.5
Alper, H.S.6
-
160
-
-
84959063951
-
The Ty1 LTR-retrotransposon of budding yeast, Saccharomyces cerevisiae
-
Curcio, M.J., Lutz, S., Lesage, P., The Ty1 LTR-retrotransposon of budding yeast, Saccharomyces cerevisiae. Microbiol Spectr 3 (2015), 1–35, 10.1128/microbiolspec.MDNA3-0053-2014.
-
(2015)
Microbiol Spectr
, vol.3
, pp. 1-35
-
-
Curcio, M.J.1
Lutz, S.2
Lesage, P.3
-
161
-
-
0035368427
-
DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1
-
Boutabout, M., DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1. Nucleic Acids Res 29 (2001), 2217–2222, 10.1093/nar/29.11.2217.
-
(2001)
Nucleic Acids Res
, vol.29
, pp. 2217-2222
-
-
Boutabout, M.1
-
162
-
-
0026082573
-
Single-step selection for Ty1 element retrotransposition
-
Curcio, M.J., Garfinkel, D.J., Single-step selection for Ty1 element retrotransposition. Proc Natl Acad Sci 88 (1991), 936–940, 10.1073/pnas.88.3.936.
-
(1991)
Proc Natl Acad Sci
, vol.88
, pp. 936-940
-
-
Curcio, M.J.1
Garfinkel, D.J.2
-
163
-
-
0029879536
-
Integration of the yeast retrotransposon Ty1 is targeted to regions upstream of genes transcribed by RNA polymerase III
-
Devine, S.E., Boeke, J.D., Integration of the yeast retrotransposon Ty1 is targeted to regions upstream of genes transcribed by RNA polymerase III. Genes Dev 10 (1996), 620–633, 10.1101/gad.10.5.620.
-
(1996)
Genes Dev
, vol.10
, pp. 620-633
-
-
Devine, S.E.1
Boeke, J.D.2
-
164
-
-
84870585039
-
Evolutionary genomics of transposable elements in Saccharomyces cerevisiae
-
Carr, M., Bensasson, D., Bergman, C.M., Evolutionary genomics of transposable elements in Saccharomyces cerevisiae. PLoS One, 2012, 7, 10.1371/journal.pone.0050978.
-
(2012)
PLoS One
, pp. 7
-
-
Carr, M.1
Bensasson, D.2
Bergman, C.M.3
-
165
-
-
84910594404
-
Genomically encoded analog memory with precise in vivo DNA writing in living cell populations
-
Farzadfard, F., Lu, T.K., Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science (80-), 346, 2014, 1256272, 10.1126/science.1256272.
-
(2014)
Science (80-)
, vol.346
, pp. 1256272
-
-
Farzadfard, F.1
Lu, T.K.2
-
166
-
-
84891710947
-
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
-
Cho, S.W., Kim, S., Kim, Y., Kweon, J., Kim, H.S., Bae, S., et al. Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Res 24 (2014), 132–141, 10.1101/gr.162339.113.
-
(2014)
Genome Res
, vol.24
, pp. 132-141
-
-
Cho, S.W.1
Kim, S.2
Kim, Y.3
Kweon, J.4
Kim, H.S.5
Bae, S.6
-
167
-
-
23444441147
-
Gene targeting using zinc finger nucleases
-
Porteus, M.H., Carroll, D., Gene targeting using zinc finger nucleases. Nat Biotechnol 23 (2005), 967–973, 10.1038/nbt1125.
-
(2005)
Nat Biotechnol
, vol.23
, pp. 967-973
-
-
Porteus, M.H.1
Carroll, D.2
-
168
-
-
84971006562
-
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
-
Komor, A.C., Kim, Y.B., Packer, M.S., Zuris, J.A., Liu, D.R., Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 61 (2016), 5985–5991, 10.1038/nature17946.
-
(2016)
Nature
, vol.61
, pp. 5985-5991
-
-
Komor, A.C.1
Kim, Y.B.2
Packer, M.S.3
Zuris, J.A.4
Liu, D.R.5
|