Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR

Biotechnology and Bioengineering

Volumn 112, Issue 11, 2015, Pages 2406-2411

  Tsai, Ching Sung ^a   Kong, In Iok ^a   Lesmana, Anastashia ^a   Million, Gyver ^a   Zhang, Guo Chang ^a   Kim, Soo Rin ^a   Jin, Yong Su ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Cas9; Marker free engineered yeast; Xylose fermentation

Indexed keywords

ANTIBIOTICS; BIOMARKERS; DRUG THERAPY; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENES; NUCLEIC ACIDS; SUGAR SUBSTITUTES;

ANTIBIOTIC RESISTANCE GENES; CAS9; DRUG RESISTANCE GENES; HOMOLOGOUS RECOMBINATION; MARKER-FREE; SCHEFFERSOMYCES STIPITIS; XYLOSE FERMENTATION; XYLOSE-FERMENTING YEASTS;

YEAST;

ALD6 PROTEIN; CAS9 PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; DNA FRAGMENT; FUNGAL PROTEIN; GUIDE RNA; ISOMERASE; PHO13 PROTEIN; UNCLASSIFIED DRUG; XYLITOL DEHYDROGENASE; XYLOSE; XYLOSE REDUCTASE; XYLULOKINASE; RECOMBINANT PROTEIN;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA REPAIR; DOUBLE STRANDED DNA BREAK; FERMENTATION; FUNGAL GENE; FUNGAL STRAIN; GENE DELETION; GENE EXPRESSION; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; HOMOLOGOUS RECOMBINATION; NONHUMAN; PLASMID; SACCHAROMYCES CEREVISIAE; SCHEFFERSOMYCES STIPITIS; YEAST; ESCHERICHIA COLI; GENE TARGETING; GENETIC RECOMBINATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES; SACCHAROMYCETALES; TIME FACTOR;

CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; FERMENTATION; GENE TARGETING; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; PLASMIDS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES; TIME FACTORS; XYLOSE;

EID: 84942368560     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25632     Document Type: Article

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