High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Nature

Volumn 529, Issue 7587, 2016, Pages 490-495

  Kleinstiver, Benjamin P ^a,b   Pattanayak, Vikram ^a,b   Prew, Michelle S ^a   Tsai, Shengdar Q ^a,b   Nguyen, Nhu T ^a   Zheng, Zongli ^c   Joung, J Keith ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c CITY UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CRISPR ASSOCIATED PROTEIN; DNA; GUIDE RNA; NUCLEASE; SINGLE GUIDE RNA; STREPTOCOCCUS PYOGENES CAS9 HF1 NUCLEASE; UNCLASSIFIED DRUG;

CELLS AND CELL COMPONENTS; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ANALYSIS; GENOME; MUTATION; RESEARCH WORK;

ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENETIC ASSOCIATION; HUMAN; HUMAN CELL; INDEL MUTATION; NONHUMAN; PRIORITY JOURNAL; RNA SEQUENCE; SEQUENCE ANALYSIS; STREPTOCOCCUS PYOGENES; WILD TYPE;

STREPTOCOCCUS PYOGENES;

EID: 84963941043     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature16526     Document Type: Article

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