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Volumn 36, Issue 24, 1997, Pages 2830-2832

Creation of Enantioselective Biocatalysts for Organic Chemistry by In Vitro Evolution

Author keywords

Asymmetric catalysis; Enzyme catalysis; Lipases; Mutagenesis; Screening methods

Indexed keywords


EID: 0032491867     PISSN: 05700833     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.199728301     Document Type: Article
Times cited : (307)

References (51)
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    • See for example a) D. J. Berrisford, C. Bolm, K. B. Sharpless, Angew. Chem. 1995, 107, 1159-1171 ; Angew. Chem., Int. Ed. Engl. 1995, 34, 1059-1070; b) R. Noyori, Asymmetric Catalysis in Organic Synthesis, Wiley, New York, 1994; c) A. Pfaltz, Acc. Chem. Res. 1993, 26, 339-345.
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    • See for example a) D. J. Berrisford, C. Bolm, K. B. Sharpless, Angew. Chem. 1995, 107, 1159-1171 ; Angew. Chem., Int. Ed. Engl. 1995, 34, 1059-1070; b) R. Noyori, Asymmetric Catalysis in Organic Synthesis, Wiley, New York, 1994; c) A. Pfaltz, Acc. Chem. Res. 1993, 26, 339-345.
    • (1995) Angew. Chem., Int. Ed. Engl. , vol.34 , pp. 1059-1070
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    • Wiley, New York
    • See for example a) D. J. Berrisford, C. Bolm, K. B. Sharpless, Angew. Chem. 1995, 107, 1159-1171 ; Angew. Chem., Int. Ed. Engl. 1995, 34, 1059-1070; b) R. Noyori, Asymmetric Catalysis in Organic Synthesis, Wiley, New York, 1994; c) A. Pfaltz, Acc. Chem. Res. 1993, 26, 339-345.
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    • Noyori, R.1
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    • See for example a) D. J. Berrisford, C. Bolm, K. B. Sharpless, Angew. Chem. 1995, 107, 1159-1171 ; Angew. Chem., Int. Ed. Engl. 1995, 34, 1059-1070; b) R. Noyori, Asymmetric Catalysis in Organic Synthesis, Wiley, New York, 1994; c) A. Pfaltz, Acc. Chem. Res. 1993, 26, 339-345.
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    • note
    • [6a,9] using the following primers: A: 5′-GCG CAA TTA ACC CTC ACT AAA GGG AAC AAA-3′ and B: 5′-GCG TAA TAC GAC TCA CTA TAG GGC GAA-3′ PCR was performed under the following conditions: 2 min at 98°C(1 × ): 1 min at 94°C,2 min at 64°C, 1 min at 72°C (25 ×); 7 min 72°C (1 × );
  • 36
    • 0029947989 scopus 로고    scopus 로고
    • b) PCR products were ligated into plasmid pUCPL6A derived from pUCPKS (A. A. Watson, R. A. Alm, J. S. Mattick, Gene 1996, 172, 163-164). Plasmids were propagated in E. coli JM 109, isolated, and used to transform P. aeruginosa PABST7.1 ( K.-E. Jaeger, B. Schneidinger, F. Rosenau, M. Werner, D. Lang, B. W. Dijkstra, K. Schimossek, A. Zonta, M. T. Reetz, J. Mol. Catal. B: Enzym. 1997, 3, 3-12). For each new cycle of mutagenesis the lipase gene of the best perfoming clone for a given mutant generation was isolated and recloned into pBluescript II KS.
    • (1996) Gene , vol.172 , pp. 163-164
    • Watson, A.A.1    Alm, R.A.2    Mattick, J.S.3
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    • b) PCR products were ligated into plasmid pUCPL6A derived from pUCPKS (A. A. Watson, R. A. Alm, J. S. Mattick, Gene 1996, 172, 163-164). Plasmids were propagated in E. coli JM 109, isolated, and used to transform P. aeruginosa PABST7.1 ( K.-E. Jaeger, B. Schneidinger, F. Rosenau, M. Werner, D. Lang, B. W. Dijkstra, K. Schimossek, A. Zonta, M. T. Reetz, J. Mol. Catal. B: Enzym. 1997, 3, 3-12). For each new cycle of mutagenesis the lipase gene of the best perfoming clone for a given mutant generation was isolated and recloned into pBluescript II KS.
    • (1997) J. Mol. Catal. B: Enzym. , vol.3 , pp. 3-12
    • Jaeger, K.-E.1    Schneidinger, B.2    Rosenau, F.3    Werner, M.4    Lang, D.5    Dijkstra, B.W.6    Schimossek, K.7    Zonta, A.8    Reetz, M.T.9
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    • note
    • Our current strategy of using the best mutant of a given generation is not necessarily optimal. It is conceivable that a less selective mutant may lead to a better result in the subsequent mutagenesis round.
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    • 0000760617 scopus 로고
    • b) F. Theil, Chem. Rev. 1995, 95, 2203-2227;
    • (1995) Chem. Rev. , vol.95 , pp. 2203-2227
    • Theil, F.1
  • 44
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    • c) Lipases, Part A: Biotechnology (Eds.: B. Rubin, E. A. Dennis). Academic Press, San Diego, 1997 (Methods Enzymol. 1997, 284);
    • (1997) Methods Enzymol. , vol.284
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    • d) Lipases, Part B: Enzyme Characterization and Utilization (Eds.: B. Rubin, E. A. Dennis), Academic Press. San Diego, 1997 (Methods Enzymol. 1997, 286);
    • (1997) Methods Enzymol. , vol.286
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    • (Eds.: P. Woolley, S. B. Petersen), Cambridge University Press. Cambridge
    • e) E. N. Vulfson in Lipases (Eds.: P. Woolley, S. B. Petersen), Cambridge University Press. Cambridge, 1994, pp. 271-288;
    • (1994) Lipases , pp. 271-288
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    • f) Engineering of/with Lipases (Ed.: F. X. Malcata), Kluwer, Dordrecht, 1996 (NATO ASI Ser. Ser. E. 1996, 317).
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    • note
    • In our studies this also occurred to some extent, since the mutants having a low activity (which in some cases may lead to higher enantioselectivities) are "sorted out" upon screening. Only those mutants are considered which induce a notable conversion within ten minutes under the reaction conditions. Indeed, many mutants show practically no activity, which is not surprising.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.