Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 34, 2015, Pages E4689-E4696

  Huang, Mingtao ^a   Bai, Yunpeng ^b,d   Sjostrom, Staffan L ^b   Hallström, Björn M ^b   Liu, Zihe ^a,e   Petranovic, Dina ^a   Uhlén, Mathias ^b,c   Joensson, Haakan N ^b   Andersson Svahn, Helene ^b   Nielsen, Jens ^a,b,c  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^c Chalmers University of Technology   (Denmark)

^d EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^e INSTITUTE OF CHEMICAL AND ENGINEERING SCIENCES   (Singapore)

Author keywords

Droplet microfluidics; Protein secretion; Random mutagenesis; Systems biology; Yeast cell factories

Indexed keywords

AMYLASE; BETA FRUCTOFURANOSIDASE; FUNGAL ENZYME; GLUCAN 1,4 ALPHA GLUCOSIDASE; SACCHAROMYCES CEREVISIAE PROTEIN;

AMYLASE RELEASE; ARTICLE; CELL CLONE; CELL POPULATION; CHROMOSOME 3; CHROMOSOME DUPLICATION; CHROMOSOME SUBSTITUTION; CONTROLLED STUDY; ENDOCYTOSIS; ENZYME ACTIVITY; ENZYME SYNTHESIS; FUNGAL GENE; FUNGAL STRAIN; GENE DELETION; GENE MUTATION; GENE ONTOLOGY; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC IDENTIFICATION; GENETIC SCREENING; GENETIC STABILITY; GENETIC VARIABILITY; HIGH THROUGHPUT SCREENING; MEMBRANE FUSION; METABOLIC ENGINEERING; MICROFLUIDICS; NONHUMAN; NONSENSE MUTATION; POINT MUTATION; PRIORITY JOURNAL; PROTEIN SECRETION; RESTRICTION MAPPING; SACCHAROMYCES CEREVISIAE; ULTRAVIOLET IRRADIATION; YEAST CELL; FUNGAL GENOME; GENETICS; MUTATION; SECRETION (PROCESS);

GENOME, FUNGAL; MICROFLUIDICS; MUTATION; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84940521020     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1506460112     Document Type: Article

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