Multiplexed in vivo his-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis

ACS Synthetic Biology

Volumn 1, Issue 2, 2012, Pages 43-52

  Wang, Harris H ^a   Huang, Po Yi ^b   Xu, George ^a,b   Haas, Wilhelm ^a   Marblestone, Adam ^a,b   Li, Jun ^c,d   Gygi, Steven P ^a   Forster, Anthony C ^d   Jewett, Michael C ^e   Church, George M ^a,b  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c HARVARD MIT DIVISION OF HEALTH SCIENCES AND TECHNOLOGY   (United States)

^d UPPSALA UNIVERSITY   (Sweden)

^e Northwestern University   (United States)

Author keywords

Cell free protein synthesis; Genome engineering; MAGE; Multienzyme catalysis; Protein purification

Indexed keywords

ELONGATION FACTOR G; ELONGATION FACTOR TS; ELONGATION FACTOR TU; INITIATION FACTOR 3; POLYHISTIDINE TAG; PROTEOME;

ARTICLE; CARBOHYDRATE SYNTHESIS; CATALYSIS; CONTROLLED STUDY; GENE INSERTION; GENE SEQUENCE; GENETIC ENGINEERING; IN VITRO STUDY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; MULTIENZYME CATALYSIS; MULTIPLEX AUTOMATED GENOME ENGINEERING; MULTIPLEX POLYMERASE CHAIN REACTION; ONE POT SYNTHESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; TRANSLATION INITIATION;

BIOTECHNOLOGY; CATALYSIS; ENZYMES; ESCHERICHIA COLI PROTEINS; HISTIDINE; METABOLIC NETWORKS AND PATHWAYS; PROTEIN ENGINEERING; SYNTHETIC BIOLOGY;

ESCHERICHIA COLI;

EID: 84863328748     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb3000029     Document Type: Article

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