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Volumn 895, Issue , 2016, Pages 187-222

Strategies to Determine Off-Target Effects of Engineered Nucleases

Author keywords

CRISPR Cas9; Gene editing; Nucleases; Off target; Specificity; TAL effector nuclease (TALEN); Zinc finger nuclease (ZFN)

Indexed keywords


EID: 85047227706     PISSN: 00652598     EISSN: 22148019     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3509-3_11     Document Type: Chapter
Times cited : (2)

References (121)
  • 1
    • 0022389290 scopus 로고
    • Insertion of DNAsequences into the human chromosomal beta-globin locus by homologous recombination
    • Smithies O, Gregg RG, Boggs SS, Koralewski MA, Kucherlapati RS. Insertion of DNAsequences into the human chromosomal beta-globin locus by homologous recombination. Nature. 1985;317:230–4.
    • (1985) Nature , vol.317 , pp. 230-234
    • Smithies, O.1    Gregg, R.G.2    Boggs, S.S.3    Koralewski, M.A.4    Kucherlapati, R.S.5
  • 2
    • 0024328536 scopus 로고
    • Altering the genome by homologous recombination
    • Capecchi MR. Altering the genome by homologous recombination. Science. 1989;244:1288–92.
    • (1989) Science , vol.244 , pp. 1288-1292
    • Capecchi, M.R.1
  • 4
    • 79951694132 scopus 로고    scopus 로고
    • Meganucleases and other tools for targeted genome engineering: Perspectives and challenges for gene therapy
    • Silva G, Poirot L, Galetto R, Smith J, Montoya G, Duchateau P, Pâques F. Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy. Curr Gene Ther. 2011;11:11–27.
    • (2011) Curr Gene Ther , vol.11 , pp. 11-27
    • Silva, G.1    Poirot, L.2    Galetto, R.3    Smith, J.4    Montoya, G.5    Duchateau, P.6    Pâques, F.7
  • 5
    • 23444441147 scopus 로고    scopus 로고
    • Gene targeting using zinc finger nucleases
    • Porteus MH, Carroll D. Gene targeting using zinc finger nucleases. Nat Biotechnol. 2005;23:967–73.
    • (2005) Nat Biotechnol , vol.23 , pp. 967-973
    • Porteus, M.H.1    Carroll, D.2
  • 7
    • 0032876974 scopus 로고    scopus 로고
    • Chimeric restriction enzymes: What is next?
    • Chandrasegaran S, Smith J. Chimeric restriction enzymes: what is next? Biol Chem. 1999;380:841–8.
    • (1999) Biol Chem , vol.380 , pp. 841-848
    • Chandrasegaran, S.1    Smith, J.2
  • 11
    • 72149090954 scopus 로고    scopus 로고
    • A simple cipher governs DNA recognition by TAL effectors
    • Moscou MJ, Bogdanove AJ. A simple cipher governs DNA recognition by TAL effectors. Science. 2009;326:1501.
    • (2009) Science , vol.326 , pp. 1501
    • Moscou, M.J.1    Bogdanove, A.J.2
  • 13
  • 14
  • 15
    • 77952008829 scopus 로고    scopus 로고
    • Frequent endonuclease cleavage at off-target locations in vivo
    • Petek LM, Russell DW, Miller DG. Frequent endonuclease cleavage at off-target locations in vivo. Mol Ther. 2010;18:983–6.
    • (2010) Mol Ther , vol.18 , pp. 983-986
    • Petek, L.M.1    Russell, D.W.2    Miller, D.G.3
  • 16
    • 46749157875 scopus 로고    scopus 로고
    • Evolution of C2H2-zinc finger genes and subfamilies in mammals: Species-specific duplication and loss of clusters, genes and effector domains
    • Tadepally HD, Burger G, Aubry M. Evolution of C2H2-zinc finger genes and subfamilies in mammals: species-specific duplication and loss of clusters, genes and effector domains. BMC Evol Biol. 2008;8:176.
    • (2008) BMC Evol Biol , vol.8
    • Tadepally, H.D.1    Burger, G.2    Aubry, M.3
  • 17
    • 0040215628 scopus 로고
    • Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes
    • Miller J, McLachlan AD, Klug A. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J. 1985;4:1609–14.
    • (1985) EMBO J , vol.4 , pp. 1609-1614
    • Miller, J.1    McLachlan, A.D.2    Klug, A.3
  • 18
    • 0023375317 scopus 로고
    • Metal-dependent folding of a single zinc finger from transcription factor IIIA
    • Frankel AD, Berg JM, Pabo CO. Metal-dependent folding of a single zinc finger from transcription factor IIIA. Proc Natl Acad Sci U S A. 1987;84:4841–5.
    • (1987) Proc Natl Acad Sci U S A , vol.84 , pp. 4841-4845
    • Frankel, A.D.1    Berg, J.M.2    Pabo, C.O.3
  • 19
    • 0034890853 scopus 로고    scopus 로고
    • Beyond the "recognition code": Structures of two Cys2His2 zinc finger/TATA box complexes
    • Wolfe SA, Grant RA, Elrod-Erickson M, Pabo CO. Beyond the "recognition code": structures of two Cys2His2 zinc finger/TATA box complexes. Structure. 2001;9:717–23.
    • (2001) Structure , vol.9 , pp. 717-723
    • Wolfe, S.A.1    Grant, R.A.2    Elrod-Erickson, M.3    Pabo, C.O.4
  • 21
  • 22
    • 0030032063 scopus 로고    scopus 로고
    • Hybrid restriction enzymes: Zinc finger fusions to Fok I cleavage domain
    • Kim YG, Cha J, Chandrasegaran S. Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc Natl Acad Sci U S A. 1996;93:1156–60.
    • (1996) Proc Natl Acad Sci U S A , vol.93 , pp. 1156-1160
    • Kim, Y.G.1    Cha, J.2    Chandrasegaran, S.3
  • 24
    • 0038523969 scopus 로고    scopus 로고
    • Chimeric nucleases stimulate gene targeting in human cells
    • Porteus MH, Baltimore D. Chimeric nucleases stimulate gene targeting in human cells. Science. 2003;300:763.
    • (2003) Science , vol.300 , pp. 763
    • Porteus, M.H.1    Baltimore, D.2
  • 25
    • 84864439768 scopus 로고    scopus 로고
    • Targeted gene knockout by direct delivery of zincfinger nuclease proteins
    • Gaj T, Guo J, Kato Y, Sirk SJ, Iii CFB. Targeted gene knockout by direct delivery of zincfinger nuclease proteins. Nat Methods. 2012;9:805–7.
    • (2012) Nat Methods , vol.9 , pp. 805-807
    • Gaj, T.1    Guo, J.2    Kato, Y.3    Sirk, S.J.4    Iii, C.F.B.5
  • 27
    • 80052293623 scopus 로고    scopus 로고
    • Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection
    • Pattanayak V, Ramirez CL, Joung JK, Liu DR. Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat Methods. 2011;8:765–70.
    • (2011) Nat Methods , vol.8 , pp. 765-770
    • Pattanayak, V.1    Ramirez, C.L.2    Joung, J.K.3    Liu, D.R.4
  • 29
    • 78651341148 scopus 로고    scopus 로고
    • Zinc finger protein-dependent and-independent contributions to the in vivo off-target activity of zinc finger nucleases
    • Gupta A, Meng X, Zhu LJ, Lawson ND, Wolfe SA. Zinc finger protein-dependent and-independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res. 2011;39:381–92.
    • (2011) Nucleic Acids Res , vol.39 , pp. 381-392
    • Gupta, A.1    Meng, X.2    Zhu, L.J.3    Lawson, N.D.4    Wolfe, S.A.5
  • 31
    • 0030587774 scopus 로고    scopus 로고
    • Zif268 protein-DNA complex refined at 1.6 A: A model system for understanding zinc finger-DNA interactions
    • Elrod-Erickson M, Rould MA, Nekludova L, Pabo CO. Zif268 protein-DNA complex refined at 1.6 A: a model system for understanding zinc finger-DNA interactions. Structure. 1996;4:1171–80.
    • (1996) Structure , vol.4 , pp. 1171-1180
    • Elrod-Erickson, M.1    Rould, M.A.2    Nekludova, L.3    Pabo, C.O.4
  • 32
    • 0028046895 scopus 로고
    • Selection of DNA binding sites for zinc fingers using rationally randomized DNA reveals coded interactions
    • Choo Y, Klug A. Selection of DNA binding sites for zinc fingers using rationally randomized DNA reveals coded interactions. Proc Natl Acad Sci U S A. 1994;91:11168–72.
    • (1994) Proc Natl Acad Sci U S A , vol.91 , pp. 11168-11172
    • Choo, Y.1    Klug, A.2
  • 33
    • 0028227930 scopus 로고
    • In vitro selection of zinc fingers with altered DNA-binding specificity
    • Jamieson AC, Kim SH, Wells JA. In vitro selection of zinc fingers with altered DNA-binding specificity. Biochemistry. 1994;33:5689–95.
    • (1994) Biochemistry , vol.33 , pp. 5689-5695
    • Jamieson, A.C.1    Kim, S.H.2    Wells, J.A.3
  • 34
    • 0028328872 scopus 로고
    • Zinc finger phage: Affinity selection of fingers with new DNA-binding specificities
    • Rebar EJ, Pabo CO. Zinc finger phage: affinity selection of fingers with new DNA-binding specificities. Science. 1994;263:671–3.
    • (1994) Science , vol.263 , pp. 671-673
    • Rebar, E.J.1    Pabo, C.O.2
  • 35
    • 0027994717 scopus 로고
    • Toward a code for the interactions of zinc fingers with DNA: Selection of randomized fingers displayed on phage
    • Choo Y, Klug A. Toward a code for the interactions of zinc fingers with DNA: selection of randomized fingers displayed on phage. Proc Natl Acad Sci U S A. 1994;91:11163–7.
    • (1994) Proc Natl Acad Sci U S A , vol.91 , pp. 11163-11167
    • Choo, Y.1    Klug, A.2
  • 36
    • 0034691202 scopus 로고    scopus 로고
    • A bacterial two-hybrid selection system for studying protein DNA and protein-protein interactions
    • Joung JK, Ramm EI, Pabo CO. A bacterial two-hybrid selection system for studying protein DNA and protein-protein interactions. Proc Natl Acad Sci U S A. 2000;97:7382–7.
    • (2000) Proc Natl Acad Sci U S A , vol.97 , pp. 7382-7387
    • Joung, J.K.1    Ramm, E.I.2    Pabo, C.O.3
  • 37
    • 0036160173 scopus 로고    scopus 로고
    • Identifying and modifying protein-DNA and protein-protein interactions using a bacterial two-hybrid selection system
    • Joung JK. Identifying and modifying protein-DNA and protein-protein interactions using a bacterial two-hybrid selection system. J Cell Biochem Suppl. 2001;37:53–7.
    • (2001) J Cell Biochem Suppl , vol.37 , pp. 53-57
    • Joung, J.K.1
  • 41
    • 0035852693 scopus 로고    scopus 로고
    • Design of polyzinc finger peptides with structured linkers
    • Moore M, Choo Y, Klug A. Design of polyzinc finger peptides with structured linkers. Proc Natl Acad Sci U S A. 2001;98:1432–6.
    • (2001) Proc Natl Acad Sci U S A , vol.98 , pp. 1432-1436
    • Moore, M.1    Choo, Y.2    Klug, A.3
  • 42
    • 0034661843 scopus 로고    scopus 로고
    • Combining structure-based design with phage display to create new Cys(2)His(2) zinc finger dimers
    • Wolfe SA, Ramm EI, Pabo CO. Combining structure-based design with phage display to create new Cys(2)His(2) zinc finger dimers. Structure. 2000;8:739–50.
    • (2000) Structure , vol.8 , pp. 739-750
    • Wolfe, S.A.1    Ramm, E.I.2    Pabo, C.O.3
  • 43
    • 0034681918 scopus 로고    scopus 로고
    • DNA-bending finger: Artificial design of 6-zinc finger peptides with polyglycine linker and induction of DNA bending
    • Imanishi M, Hori Y, Nagaoka M, Sugiura Y. DNA-bending finger: artificial design of 6-zinc finger peptides with polyglycine linker and induction of DNA bending. Biochemistry. 2000;39:4383–90.
    • (2000) Biochemistry , vol.39 , pp. 4383-4390
    • Imanishi, M.1    Hori, Y.2    Nagaoka, M.3    Sugiura, Y.4
  • 45
    • 84898998318 scopus 로고    scopus 로고
    • An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage
    • Fine EJ, Cradick TJ, Zhao CL, Lin Y, Bao G. An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage. Nucleic Acids Res. 2014;42, e42.
    • (2014) Nucleic Acids Res , vol.42 , pp. e42
    • Fine, E.J.1    Cradick, T.J.2    Zhao, C.L.3    Lin, Y.4    Bao, G.5
  • 47
    • 0027327521 scopus 로고
    • A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA
    • Choo Y, Klug A. A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA. Nucleic Acids Res. 1993;21:3341–6.
    • (1993) Nucleic Acids Res , vol.21 , pp. 3341-3346
    • Choo, Y.1    Klug, A.2
  • 48
    • 2442684204 scopus 로고    scopus 로고
    • Reduction in DNA-binding affinity of Cys2His2 zinc finger proteins by linker phosphorylation
    • Jantz D, Berg JM. Reduction in DNA-binding affinity of Cys2His2 zinc finger proteins by linker phosphorylation. Proc Natl Acad Sci U S A. 2004;101:7589–93.
    • (2004) Proc Natl Acad Sci U S A , vol.101 , pp. 7589-7593
    • Jantz, D.1    Berg, J.M.2
  • 49
    • 58149265244 scopus 로고    scopus 로고
    • Expanding or restricting the target site repertoire of zincfinger nucleases: The inter-domain linker as a major determinant of target site selectivity
    • Handel EM, Alwin S, Cathomen T. Expanding or restricting the target site repertoire of zincfinger nucleases: the inter-domain linker as a major determinant of target site selectivity. Mol Ther. 2009;17:104–11.
    • (2009) Mol Ther , vol.17 , pp. 104-111
    • Handel, E.M.1    Alwin, S.2    Cathomen, T.3
  • 50
    • 30944459858 scopus 로고    scopus 로고
    • Gene-for-gene-mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins
    • Schornack S, Meyer A, Römer P, Jordan T, Lahaye T. Gene-for-gene-mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins. J Plant Physiol. 2006;163:256–72.
    • (2006) J Plant Physiol , vol.163 , pp. 256-272
    • Schornack, S.1    Meyer, A.2    Römer, P.3    Jordan, T.4    Lahaye, T.5
  • 51
    • 77956126894 scopus 로고    scopus 로고
    • Xanthomonas AvrBs3 family-type III effectors: Discovery and function
    • Boch J, Bonas U. Xanthomonas AvrBs3 family-type III effectors: discovery and function. Annu Rev Phytopathol. 2009;48:419–36.
    • (2009) Annu Rev Phytopathol , vol.48 , pp. 419-436
    • Boch, J.1    Bonas, U.2
  • 55
    • 80053039555 scopus 로고    scopus 로고
    • A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
    • Mussolino C, Morbitzer R, Lutge F, Dannemann N, Lahaye T, Cathomen T. A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res. 2011;39:9283–93.
    • (2011) Nucleic Acids Res , vol.39 , pp. 9283-9293
    • Mussolino, C.1    Morbitzer, R.2    Lutge, F.3    Dannemann, N.4    Lahaye, T.5    Cathomen, T.6
  • 57
    • 84897954502 scopus 로고    scopus 로고
    • Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity
    • Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, Liu DR. Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity. Nat Methods. 2014;11:429–35.
    • (2014) Nat Methods , vol.11 , pp. 429-435
    • Guilinger, J.P.1    Pattanayak, V.2    Reyon, D.3    Tsai, S.Q.4    Sander, J.D.5    Joung, J.K.6    Liu, D.R.7
  • 60
    • 34447323568 scopus 로고    scopus 로고
    • Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases
    • Szczepek M, Brondani V, Buchel J, Serrano L, Segal DJ, Cathomen T. Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases. Nat Biotechnol. 2007;25:786–93.
    • (2007) Nat Biotechnol , vol.25 , pp. 786-793
    • Szczepek, M.1    Brondani, V.2    Buchel, J.3    Serrano, L.4    Segal, D.J.5    Cathomen, T.6
  • 64
    • 77953702888 scopus 로고    scopus 로고
    • Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases
    • Guo J, Gaj T, Barbas 3rd CF. Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases. J Mol Biol. 2010;400:96–107.
    • (2010) J Mol Biol , vol.400 , pp. 96-107
    • Guo, J.1    Gaj, T.2    Barbas, C.F.3
  • 66
    • 23844505202 scopus 로고    scopus 로고
    • Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin
    • Bolotin A, Quinquis B, Sorokin A, Ehrlich SD. Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin. Microbiology. 2005;151:2551–61.
    • (2005) Microbiology , vol.151 , pp. 2551-2561
    • Bolotin, A.1    Quinquis, B.2    Sorokin, A.3    Ehrlich, S.D.4
  • 67
    • 74249095519 scopus 로고    scopus 로고
    • CRISPR/Cas, the immune system of bacteria and archaea
    • Horvath P, Barrangou R. CRISPR/Cas, the immune system of bacteria and archaea. Science. 2010;327:167–70.
    • (2010) Science , vol.327 , pp. 167-170
    • Horvath, P.1    Barrangou, R.2
  • 68
    • 77249170201 scopus 로고    scopus 로고
    • CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea
    • Marraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet. 2010;11:181–90.
    • (2010) Nat Rev Genet , vol.11 , pp. 181-190
    • Marraffini, L.A.1    Sontheimer, E.J.2
  • 71
    • 84884325112 scopus 로고    scopus 로고
    • Genome engineering at the dawn of the golden age
    • Segal DJ, Meckler JF. Genome engineering at the dawn of the golden age. Annu Rev Genomics Hum Genet. 2013;14:135–58.
    • (2013) Annu Rev Genomics Hum Genet , vol.14 , pp. 135-158
    • Segal, D.J.1    Meckler, J.F.2
  • 72
    • 84866859751 scopus 로고    scopus 로고
    • Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
    • Gasiunas G, Barrangou R, Horvath P, Siksnys V. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A. 2012;109:E2579–86.
    • (2012) Proc Natl Acad Sci U S A , vol.109 , pp. E2579-E2586
    • Gasiunas, G.1    Barrangou, R.2    Horvath, P.3    Siksnys, V.4
  • 74
    • 77957756454 scopus 로고    scopus 로고
    • Quantification of zinc finger nuclease-associated toxicity
    • Cornu TI, Cathomen T. Quantification of zinc finger nuclease-associated toxicity. Methods Mol Biol. 2010;649:237–45.
    • (2010) Methods Mol Biol , vol.649 , pp. 237-245
    • Cornu, T.I.1    Cathomen, T.2
  • 77
    • 84884950106 scopus 로고    scopus 로고
    • CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
    • Cradick TJ, Fine EJ, Antico CJ, Bao G. CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res. 2013;41:9584–92.
    • (2013) Nucleic Acids Res , vol.41 , pp. 9584-9592
    • Cradick, T.J.1    Fine, E.J.2    Antico, C.J.3    Bao, G.4
  • 79
  • 80
    • 84879026965 scopus 로고    scopus 로고
    • Protospacer recognition motifs: Mixed identities and functional diversity
    • Shah SA, Erdmann S, Mojica FJ, Garrett RA. Protospacer recognition motifs: mixed identities and functional diversity. RNA Biol. 2013;10:891–9.
    • (2013) RNA Biol , vol.10 , pp. 891-899
    • Shah, S.A.1    Erdmann, S.2    Mojica, F.J.3    Garrett, R.A.4
  • 83
    • 84891710947 scopus 로고    scopus 로고
    • Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
    • Cho SW, Kim S, Kim Y, Kweon J, Kim HS, Bae S, Kim J-S. Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Res. 2013;24:132–41.
    • (2013) Genome Res , vol.24 , pp. 132-141
    • Cho, S.W.1    Kim, S.2    Kim, Y.3    Kweon, J.4    Kim, H.S.5    Bae, S.6    Kim, J.-S.7
  • 87
    • 84884160273 scopus 로고    scopus 로고
    • CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
    • Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L, Church GM. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013;31:833–8.
    • (2013) Nat Biotechnol , vol.31 , pp. 833-838
    • Mali, P.1    Aach, J.2    Stranges, P.B.3    Esvelt, K.M.4    Moosburner, M.5    Kosuri, S.6    Yang, L.7    Church, G.M.8
  • 88
    • 84902210542 scopus 로고    scopus 로고
    • Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
    • Guilinger JP, Thompson DB, Liu DR. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol. 2014;32:577–82.
    • (2014) Nat Biotechnol , vol.32 , pp. 577-582
    • Guilinger, J.P.1    Thompson, D.B.2    Liu, D.R.3
  • 91
    • 84864808070 scopus 로고    scopus 로고
    • 7-Aminoactinomycin D for apoptosis staining in flow cytometry
    • Zembruski NC, Stache V, Haefeli WE, Weiss J. 7-Aminoactinomycin D for apoptosis staining in flow cytometry. Anal Biochem. 2012;429:79–81.
    • (2012) Anal Biochem , vol.429 , pp. 79-81
    • Zembruski, N.C.1    Stache, V.2    Haefeli, W.E.3    Weiss, J.4
  • 95
    • 84884155038 scopus 로고    scopus 로고
    • High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
    • Pattanayak V, Lin S, Guilinger JP, Ma E, Doudna JA, Liu DR. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol. 2013;31:839–43.
    • (2013) Nat Biotechnol , vol.31 , pp. 839-843
    • Pattanayak, V.1    Lin, S.2    Guilinger, J.P.3    Ma, E.4    Doudna, J.A.5    Liu, D.R.6
  • 96
    • 84923221641 scopus 로고    scopus 로고
    • Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
    • Wang X, Wang Y, Wu X, Wang J, Wang Y, Qiu Z, Chang T, Huang H, Lin R-J, Yee J-K. Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors. Nat Biotechnol. 2015;33:175–8.
    • (2015) Nat Biotechnol , vol.33 , pp. 175-178
    • Wang, X.1    Wang, Y.2    Wu, X.3    Wang, J.4    Wang, Y.5    Qiu, Z.6    Chang, T.7    Huang, H.8    Lin, R.-J.9    Yee, J.-K.10
  • 98
    • 84938836171 scopus 로고    scopus 로고
    • A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture
    • O’Geen H, Henry IM, Bhakta MS, Meckler JF, Segal DJ. A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture. Nucleic Acids Res. 2014;43:3389–404.
    • (2014) Nucleic Acids Res , vol.43 , pp. 3389-3404
    • O’Geen, H.1    Henry, I.M.2    Bhakta, M.S.3    Meckler, J.F.4    Segal, D.J.5
  • 99
    • 84903545084 scopus 로고    scopus 로고
    • Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
    • Kuscu C, Arslan S, Singh R, Thorpe J, Adli M. Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol. 2014;32:677–83.
    • (2014) Nat Biotechnol , vol.32 , pp. 677-683
    • Kuscu, C.1    Arslan, S.2    Singh, R.3    Thorpe, J.4    Adli, M.5
  • 101
    • 84923275611 scopus 로고    scopus 로고
    • Genome-wide detection of DNA doublestranded breaks induced by engineered nucleases
    • Frock RL, Hu J, Meyers RM, Ho YJ, Kii E, Alt FW. Genome-wide detection of DNA doublestranded breaks induced by engineered nucleases. Nat Biotechnol. 2015;33:179–86.
    • (2015) Nat Biotechnol , vol.33 , pp. 179-186
    • Frock, R.L.1    Hu, J.2    Meyers, R.M.3    Ho, Y.J.4    Kii, E.5    Alt, F.W.6
  • 106
    • 84888090695 scopus 로고    scopus 로고
    • TALENoffer: Genome-wide TALEN off-target prediction
    • Grau J, Boch J, Posch S. TALENoffer: genome-wide TALEN off-target prediction. Bioinformatics. 2013;29:2931–2.
    • (2013) Bioinformatics , vol.29 , pp. 2931-2932
    • Grau, J.1    Boch, J.2    Posch, S.3
  • 108
    • 84921598229 scopus 로고    scopus 로고
    • COSMID: A Web-based tool for identifying and validating CRISPR/Cas Off-target sites
    • Cradick TJ, Qui P, Lee CM, Fine EJ, Bao G. COSMID: a Web-based tool for identifying and validating CRISPR/Cas Off-target sites. Mol Ther Nucleic Acids. 2014;3:e214.
    • (2014) Mol Ther Nucleic Acids , vol.3 , pp. e214
    • Cradick, T.J.1    Qui, P.2    Lee, C.M.3    Fine, E.J.4    Bao, G.5
  • 110
    • 84903549014 scopus 로고    scopus 로고
    • sgRNAcas9: A software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites
    • Xie S, Shen B, Zhang C, Huang X, Zhang Y. sgRNAcas9: A software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One. 2014;9:e100448.
    • (2014) Plos One , vol.9
    • Xie, S.1    Shen, B.2    Zhang, C.3    Huang, X.4    Zhang, Y.5
  • 115
    • 84886513724 scopus 로고    scopus 로고
    • A guide RNA sequence design platform for the CRISPR/ Cas9 system for model organism genomes
    • Ma M, Ye AY, Zheng W, Kong L. A guide RNA sequence design platform for the CRISPR/ Cas9 system for model organism genomes. Biomed Res Int. 2013;2013:270805.
    • (2013) Biomed Res Int , vol.2013
    • Ma, M.1    Ye, A.Y.2    Zheng, W.3    Kong, L.4
  • 120
    • 84907573408 scopus 로고    scopus 로고
    • CRISPRseek: A bioconductor package to identify target-specific guide RNAs for CRISPR-CAS9 genome-editing systems
    • Zhu LJ, Holmes BR, Aronin N, Brodsky MH. CRISPRseek: a bioconductor package to identify target-specific guide RNAs for CRISPR-CAS9 genome-editing systems. PLoS One. 2014;9, e108424.
    • (2014) Plos One , vol.9
    • Zhu, L.J.1    Holmes, B.R.2    Aronin, N.3    Brodsky, M.H.4
  • 121
    • 84896308706 scopus 로고    scopus 로고
    • Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
    • Bae S, Park J, Kim JS. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics. 2014;30:1473–5.
    • (2014) Bioinformatics , vol.30 , pp. 1473-1475
    • Bae, S.1    Park, J.2    Kim, J.S.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.