Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 1, 2015, Pages 112-117

  Guntas, Gurkan ^a   Hallett, Ryan A ^a   Zimmerman, Seth P ^a   Williams, Tishan ^a   Yumerefendi, Hayretin ^a   Bear, James E ^a,b   Kuhlman, Brian ^a,b  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

Computational library; Optogenetic tool; Per arnt sim domain; Phage display; Rosetta molecular modeling suite

Indexed keywords

DIMER; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATASE; IMPROVED LIGHT INDUCED DIMER; UNCLASSIFIED DRUG; MUTANT PROTEIN; VEGETABLE PROTEIN;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING ASSAY; BLUE LIGHT; CELL CULTURE; CELLULAR DISTRIBUTION; CRYSTAL STRUCTURE; FIBROBLAST; GENETIC TRANSFECTION; MAMMAL CELL; MOUSE; NONHUMAN; OAT; PHAGE DISPLAY; PHOTOACTIVATION; PHOTOSTIMULATION; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; SURFACE PROPERTY; AMINO ACID SEQUENCE; AVENA; CELL FRACTIONATION; CELL SURFACE DISPLAY; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; LIGHT; METABOLISM; MOLECULAR GENETICS; PROTEIN MULTIMERIZATION; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT; RADIATION RESPONSE;

AVENA SATIVA; BACTERIA (MICROORGANISMS); MAMMALIA;

AMINO ACID SEQUENCE; AVENA; CELL SURFACE DISPLAY TECHNIQUES; CELLS, CULTURED; ENZYME-LINKED IMMUNOSORBENT ASSAY; GTP PHOSPHOHYDROLASES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; LIGHT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PLANT PROTEINS; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; SUBCELLULAR FRACTIONS;

EID: 84920409500     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1417910112     Document Type: Article

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