Direct mutagenesis of thousands of genomic targets using microarray-derived oligonucleotides

ACS Synthetic Biology

Volumn 4, Issue 1, 2015, Pages 17-22

  Bonde, Mads T ^a,b   Kosuri, Sriram ^c   Genee, Hans J ^a,b   Sarup Lytzen, Kira ^a,b   Church, George M ^d,e   Sommer, Morten O A ^a,b   Wang, Harris H ^f  

^a Chalmers University of Technology   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d HARVARD UNIVERSITY   (United States)

^e HARVARD MEDICAL SCHOOL   (United States)

^f Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

Genome engineering; Library synthesis; MAGE; Metabolic engineering; Microarray

Indexed keywords

OLIGONUCLEOTIDE; PHOSPHORAMIDOUS ACID;

3' UNTRANSLATED REGION; ACRA GENE; ACRD GENE; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; DNA SEQUENCE; EDD GENE; ESCHERICHIA COLI; FRYB GENE; GENE INSERTION SEQUENCE; GLND GENE; HEMC GENE; HIGH THROUGHPUT SEQUENCING; INDEL MUTATION; MDAB GENE; METABOLIC ENGINEERING; MUTAGENESIS; OPERON; OSMC GENE; PRIORITY JOURNAL; PROMOTER REGION; PSSA GENE; SECE GENE; SODB GENE; THRL GENE; DNA MICROARRAY; ENTEROBACTERIA PHAGE T7; GENE LIBRARY; GENETIC ENGINEERING; GENETICS; PROCEDURES; SYNTHETIC BIOLOGY;

BACTERIA (MICROORGANISMS);

BACTERIOPHAGE T7; ESCHERICHIA COLI; GENE LIBRARY; GENETIC ENGINEERING; GENOME, BACTERIAL; METABOLIC ENGINEERING; MUTAGENESIS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OPERON; PROMOTER REGIONS, GENETIC; SYNTHETIC BIOLOGY;

EID: 84921417685     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb5001565     Document Type: Article

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