Combinatorial Evolution of Enzymes and Synthetic Pathways Using One-Step PCR

ACS Synthetic Biology

Volumn 5, Issue 3, 2016, Pages 259-268

  Jin, Peng ^a   Kang, Zhen ^a   Zhang, Junli ^a   Zhang, Linpei ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

combinatorial mutagenesis; directed evolution; pathway optimization; single stranded DNA oligonucleotides; synthetic biology

Indexed keywords

AMINOLEVULINIC ACID; DNA; DNA LIGASE; DNA POLYMERASE; ENZYME; HEME; HEPARIN LYASE; HYALURONIDASE; OLIGONUCLEOTIDE; SIGMA FACTOR RPOS; DNA DIRECTED DNA POLYMERASE; HYALURONOGLUCOSAMINIDASE;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; COMBINATORIAL LIBRARY; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SPECIFICITY; ENZYME SYNTHESIS; KOMAGATAELLA PASTORIS; MUTANT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; RAPIDLY EFFICIENT COMBINATORIAL OLIGONUCLEOTIDES FOR DIRECTED EVOLUTION; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; AMINO ACID SEQUENCE; ANIMAL; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; LEECH; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; DNA LIGASES; DNA-DIRECTED DNA POLYMERASE; GENETIC ENGINEERING; HEPARIN LYASE; HYALURONOGLUCOSAMINIDASE; LEECHES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; SUBSTRATE SPECIFICITY;

EID: 84961794996     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.5b00240     Document Type: Article

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