Determination of binding affinity upon mutation for type I dockerin–cohesin complexes from Clostridium thermocellum and Clostridium cellulolyticum using deep sequencing

Proteins: Structure, Function and Bioinformatics

Volumn 84, Issue 12, 2016, Pages 1914-1928

  Kowalsky, Caitlin A ^a   Whitehead, Timothy A ^a,b  

^a Michigan State University   (United States)

^b Michigan State University   (United States)

Author keywords

cellulosomes; computational protein protein interface prediction; deep mutational scanning; protein benchmark set; yeast surface display

Indexed keywords

BACTERIAL PROTEIN; COHESIN; DOCKERIN TYPE I; UNCLASSIFIED DRUG; CELL CYCLE PROTEIN; MEMBRANE PROTEIN; NONHISTONE PROTEIN; PROTEIN BINDING; RECOMBINANT PROTEIN;

AREA UNDER THE CURVE; ARTICLE; BACTERIUM MUTANT; CLOSTRIDIUM CELLULOLYTICUM; CLOSTRIDIUM THERMOCELLUM; CONTROLLED STUDY; GENE MUTATION; GENE SEQUENCE; GENETIC VARIABILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RELATIVE BINDING AFFINITY; YEAST; BINDING SITE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HIGH THROUGHPUT SEQUENCING; METABOLISM; MOLECULAR CLONING; POINT MUTATION; PROTEIN DOMAIN; PROTEIN SECONDARY STRUCTURE; RECEIVER OPERATING CHARACTERISTIC; SOFTWARE; THERMODYNAMICS; TWO HYBRID SYSTEM;

AREA UNDER CURVE; BACTERIAL PROTEINS; BINDING SITES; CELL CYCLE PROTEINS; CHROMOSOMAL PROTEINS, NON-HISTONE; CLONING, MOLECULAR; CLOSTRIDIUM CELLULOLYTICUM; CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI; GENE EXPRESSION; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MEMBRANE PROTEINS; POINT MUTATION; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; ROC CURVE; SOFTWARE; THERMODYNAMICS; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 84994803492     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.25175     Document Type: Article

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