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Volumn 634, Issue , 2010, Pages 399-407

Easy two-step method for randomizing and cloning gene fragments

Author keywords

Balanced mutational spectrum; Domain mutagenesis; EZClone; GeneMorph; Megaprimer; Random mutagenesis

Indexed keywords

ARTICLE; MOLECULAR CLONING; PROTEIN ENGINEERING;

EID: 80054894865     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-652-8_28     Document Type: Article
Times cited : (2)

References (9)
  • 2
    • 0029999325 scopus 로고    scopus 로고
    • Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions
    • Vartanian JP, Henry M, Wain-Hobson S (1996) Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions. Nucleic Acids Res 24 (14): 2627-2631
    • (1996) Nucleic Acids Res , vol.24 , Issue.14 , pp. 2627-2631
    • Vartanian, J.P.1    Henry, M.2    Wain-Hobson, S.3
  • 3
    • 0029969577 scopus 로고    scopus 로고
    • Directed evolution of subtilisin e in Bacillus subtilis to enhance total activity in aqueous dimethylformamide
    • You L, Arnold FH (1996) Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide. Protein Eng 9 (1): 77-83
    • (1996) Protein Eng , vol.9 , Issue.1 , pp. 77-83
    • You, L.1    Arnold, F.H.2
  • 4
    • 0030737949 scopus 로고    scopus 로고
    • Generation of large libraries of random mutants in Bacillus subtilis by PCRbased plasmid multimerization
    • Shafikhani S, Siegel RA, Ferrari E, Schellenberger V (1997) Generation of large libraries of random mutants in Bacillus subtilis by PCRbased plasmid multimerization. Biotechniques 23 (2): 304-310
    • (1997) Biotechniques , vol.23 , Issue.2 , pp. 304-310
    • Shafikhani, S.1    Siegel, R.A.2    Ferrari, E.3    Schellenberger, V.4
  • 5
    • 0032573144 scopus 로고    scopus 로고
    • In vitro evolution of horse heart myoglobin to increase peroxidase activity
    • Wan L, Twitchett MB, Elits LD, Mauk AG, Smith M (1998) In vitro evolution of horse heart myoglobin to increase peroxidase activity. Proc Natl Acad Sci U S A 95 (22): 12825-12831
    • (1998) Proc Natl Acad Sci U S A , vol.95 , Issue.22 , pp. 12825-12831
    • Wan, L.1    Twitchett, M.B.2    Elits, L.D.3    Mauk, A.G.4    Smith, M.5
  • 7
    • 85045502002 scopus 로고
    • Randomization of genes by PCR mutagenesis
    • Cadwell RC, Joyce GF (1992) Randomization of genes by PCR mutagenesis. PCR Methods Appl 2 (1): 28-33
    • (1992) PCR Methods Appl , vol.2 , Issue.1 , pp. 28-33
    • Cadwell, R.C.1    Joyce, G.F.2
  • 8
    • 0011379580 scopus 로고    scopus 로고
    • Randomize gene sequences with new PCR mutagenesis kit
    • Cline J, Hogrefe H (2000) Randomize gene sequences with new PCR mutagenesis kit. Strategies 13 (4): 157-161
    • (2000) Strategies , vol.13 , Issue.4 , pp. 157-161
    • Cline, J.1    Hogrefe, H.2
  • 9
    • 59749085863 scopus 로고    scopus 로고
    • Novel mutations in Moloney murine leukemia virus reverse transcriptase increase thermostability through tighter binding to template-primer
    • Arezi B, Hogrefe H (2009) Novel mutations in Moloney murine leukemia virus reverse transcriptase increase thermostability through tighter binding to template-primer. Nucleic Acids Res 37 (2): 473-481
    • (2009) Nucleic Acids Res , vol.37 , Issue.2 , pp. 473-481
    • Arezi, B.1    Hogrefe, H.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.